Reagents for Immunofluorescence and Intracellular Flow Cytometry
Cell Signaling Technology offers a wide selection of secondary antibodies and control antibodies, as well as cellular dyes for immunofluorescence and intracellular flow cytometry. These same reagents are used in-house for antibody validation in flow cytometry and immunofluorescent analysis and therefore work optimally with CST's primary antibodies.
Intracellular Flow Cytometry
CST's antibodies for intracellular flow cytometry provide the researcher or clinician with new approaches to apply flow cytometry as a platform for the analysis of mechanisms underlying diseases, the identification of biomarkers and drug discovery.
Confocal immunofluorescent analysis of mouse testis
using Miwi (G82) Antibody #2079 (green) and Pan-
Keratin (C11) Mouse mAb #4545 (red). Blue
pseudocolor = DRAQ5® (fluorescent DNA dye).
| Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4803, untreated (blue) or IFN-α-treated (green). |
Alexa Fluor-Conjugated Secondary Antibodies for Flow Cytometry or Immunofluorescence
Additional Alexa Fluor-Conjugated Secondary Antibodies for Immunofluorescence
Isotype Control Antibodies
Control Antibodies for Immunofluorescence or Flow Cytometry
Additional Control Antibodies for Immunofluorescence
Additional Control Antibodies for Flow Cytometry