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Home > Products > Immunofluorescence and Flow Cytometry Reagents
 
 
Immunofluorescence and Flow Cytometry Supporting Reagents
 

Cell Signaling Technology offers a wide selection of secondary antibodies and control antibodies, as well as cellular dyes.

These same reagents are also used in-house for antibody validation in flow cytometry and immunofluorescent analysis and therefore work optimally with our primary antibodies. Technical support is provided by the product scientists who make the reagents and know them best.

 
DRAQ IF-F
DRAQ5 IF-P
Confocal immunofluorescent analysis of mouse testis
using Miwi (G82) Antibody #2079 (green) and Pan-
Keratin (C11) Mouse mAb #4545 (red). Blue
pseudocolor = DRAQ5® (fluorescent DNA dye).
Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
 
Secondary Antibodies
 
Alexa Fluor-Conjugated

Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)

Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)

Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)

Anti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)

Anti-rat IgG (H+L), (Alexa Fluor® 555 Conjugate)
Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate)
 
 
DyLight-Conjugated
 
Anti-mouse IgG (H+L) (DyLight® 680 Conjugate)
Anti-mouse IgG (H+L) (DyLight® 800 Conjugate)
Anti-rabbit IgG (H+L) (DyLight® 680 Conjugate)
Anti-rabbit IgG (H+L) (DyLight® 800 Conjugate)
 
 
Isotype Control Antibodies
 
Mouse IgG (Sepharose Bead Conjugate)
Mouse (G3A1) mAb IgG1 Isotype Control
Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate)
Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated)
Mouse (MOPC-21) mAb IgG1 Isotype control (Alexa Fluor® 647 Conjugate)
Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate)
Normal Rabbit IgG
Rabbit (DA1E) mAb IgG XP® Isotype Control
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated)
Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate)
Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
 
 
Fluorescent DNA Dyes
 
DAPI
DRAQ5®
DRAQ7™
Hoechst 33342
Propidium Iodide (PI)/RNase Staining Solution
 
 
Conjugated Antibodies for Flow Cytometry