PCR Reagents from NEB

NEB offers a wide range of DNA polymerases and through our commitment to research, ensures the development of innovative and high quality tools for PCR related applications. Several factors influence the choice of an optimal enzyme for a specific PCR application. The following chart highlights the most significant properties to be considered when choosing a DNA polymerase for PCR.

	Fidelity vs Taq	Optimal Extn Range	Difficult Templates	Resulting DNA ends	3´- 5´ exo	PCR Kit /Master Mix Available	Units /50µ Rxns	Advantages
Q5 High Fidelity DNA Polymerase	>50X	=20kb	++++	Blunt	Yes	No/Yes	1.0	Highest fidelity amplification available; ideal for routine or difficult AF and GC- rich target
Phusion High-Fidelity DNA Polymerase	>50X	=20kb	+++	Blunt	Yes	Yes/Yes	1.0	Extreme fidelity (50X greater than Taq) High speed (5X faster than Pfu) Robust reactions High yield Choice of formats and reaction buffers
Deep Vent DNA Polymerase	6X	=6kb	++	Blunt	Yes	No/No	0.5-1.0	High-fidelity (5X greater than Taq) Extremely high thermostability (half-life of 23 hr at 95°C) Ideal for GC-rich or looped sequences
Vent DNA Polymerase	5X	=6kb	+	Blunt	Yes	No/No	0.5-1.0	High-fidelity (5X greater than Taq) High thermostability (half-life of 6.7 hr at 95°C)
OneTaq DNA Polymerase	2X	=6kb	+++	3'/Blunt*	Yes	No/Yes	1.25	Ideal for routine, AT- and GC-rich PCR
Taq DNA Polymerase	1X	=5kb	-	3´A	No	Yes/Yes	1.25	Industry standard for routine PCR Choice of formats and reaction buffers to accomodate a variety of PCR applications Tolerates a wide range of templates Incorporates dUTP, dITP and flourescently-labeled nucleotides

* Sufficient 3' A ends are generated to allow for ligation to dT/dU overhang vectors.

Taq DNA Polymerase is the industry standard for routine PCR, known for robust and reliable reactions. NEB provides high quality, recombinant Taq at exceptional value in terms of cost per unit. For your convenience, this versatile enzyme is now available in a variety of formats:

Multiplex PCR 5X Master Mix	For 2 or more products in a single reaction
Taq PCR Kit	Everything you need to perform 200 PCR reactions
Taq 2X Master Mix Taq 5X Master Mix	Just add template and primers
Quick-Load Taq 2X Master Mix	Also includes dyes for tracking
Taq DNA Polymerase with ThermoPol Buffer	Uniquely formulated buffer for high product yields
Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer	Additional Mg+2 control
Taq DNA Polymerase with Standard Taq Buffer	Detergent free buffer for high throughput applications
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer	Additional Mg+2 control
Crimson Taq DNA Polymerase with Mg-Free Buffer	Mg-free Buffer contains density reagent for direct gel loading