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PCR Reagents from NEB

NEB offers a wide range of DNA polymerases and through our commitment to research, ensures the development of innovative and high quality tools for PCR related applications. Several factors influence the choice of an optimal enzyme for a specific PCR application. The following chart highlights the most significant properties to be considered when choosing a DNA polymerase for PCR.


Fidelity vs
Taq
Optimal Extn
Range
Difficult Templates Resulting DNA ends 3- 5
exo
PCR Kit
/Master Mix Available
Units
/50
Rxns
Advantages
Q5 High Fidelity DNA Polymerase

>50X

=20kb

++++

Blunt

Yes

No/Yes

1.0

Highest fidelity amplification available; ideal for routine or difficult AF and GC- rich target

Phusion High-Fidelity DNA Polymerase

>50X

=20kb

+++

Blunt

Yes

Yes/Yes

1.0

Extreme fidelity (50X greater than Taq)
High speed (5X faster than Pfu)
Robust reactions
High yield
Choice of formats and reaction buffers

Deep Vent DNA Polymerase

6X

=6kb

++

Blunt

Yes

No/No

0.5-1.0

High-fidelity (5X greater than Taq)

Extremely high thermostability
(half-life of 23 hr at 95C)
Ideal for GC-rich or looped sequences

Vent DNA Polymerase

5X

=6kb

+

Blunt

Yes

No/No

0.5-1.0

High-fidelity (5X greater than Taq)
High thermostability
(half-life of 6.7 hr at 95C)

OneTaq DNA Polymerase

2X

=6kb

+++

3'/Blunt*

Yes

No/Yes

1.25

Ideal for routine, AT- and GC-rich PCR

Taq DNA Polymerase

1X

=5kb

-

3A

No

Yes/Yes

1.25

Industry standard for routine PCR Choice of formats and reaction buffers to accomodate a variety of PCR applications Tolerates a wide range of templates Incorporates dUTP, dITP and flourescently-labeled nucleotides
 

* Sufficient 3' A ends are generated to allow for ligation to dT/dU overhang vectors.


Taq DNA Polymerase
is the industry standard for routine PCR, known for robust and reliable reactions. NEB provides high quality, recombinant Taq at exceptional value in terms of cost per unit. For your convenience, this versatile enzyme is now available in a variety of formats:

Multiplex PCR 5X Master Mix For 2 or more products in a single reaction
Taq PCR Kit Everything you need to perform 200 PCR reactions
Taq 2X Master Mix
Taq 5X Master Mix
Just add template and primers
Quick-Load Taq 2X Master Mix Also includes dyes for tracking
Taq DNA Polymerase with ThermoPol Buffer Uniquely formulated buffer for high product yields
Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer Additional Mg+2 control
Taq DNA Polymerase with Standard Taq Buffer Detergent free buffer for high throughput applications
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer Additional Mg+2 control
Crimson Taq DNA Polymerase with Mg-Free Buffer Mg-free Buffer contains density reagent for direct gel loading










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