Time-Saver™ Qualified Enzymes

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Enzymes powerful enough to digest in 5 minutes
Pure enough for overnight incubation
No special formulation
No added expense

At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction-modification systems. This focus allows us to provide extremely pure enzymes at concentrations that deliver more flexibility for your experimental design.

Whether you are quickly screening large numbers of clones, or setting up overnight digests, you will benefit from the high quality of our enzymes. Typically, a restriction digest involves the incubation of 1 µl of enzyme with 1 µg of purified DNA in a final volume of 50 µl for 1 hour. However, to speed up the screening process, choose one of NEB’s enzymes that are Time-Saver™ qualified. These enzymes will digest 1 µg of DNA in 5 minutes using 1 µl of enzyme under recommended reaction conditions. Unlike other suppliers, there is no special formulation, change in concentration or need to buy more expensive new lines of enzymes to achieve digestion in 5 minutes. In fact, 59% of our enzymes will digest 1 µg of DNA in 5 minutes, while 83% will fully digest in 15 minutes (see table). That means >180 of our restriction enzymes have the power to get the job done fast.

Power and purity of Time-Saver™ qualified enzymes: 1 µl of SalI digests 1 µg of DNA in 5 minutes with no indication of nuclease contamination in longer digests or overnight (O/N) samples. Marker (M) is the 1 kb DNA Ladder (NEB #N3232).

Also, since all of our enzymes are rigorously tested for nuclease contamination, you can safely set up digests for long periods of time without any degradation of your sample. Only NEB can offer you enzymes with power and purity - the power to digest in 5 minutes and the purity to withstand overnight digestions with no loss of sample.

Efficiency on different substrates may vary. Testing was done using the unit definition substrate (ie. Lambda DNA). Certain supercoiled plasmids may require a reduction in the amount of plasmid digested. Further information can be found on pages 259-260 of the 2005/06 NEB catalogue.


Time-Saver™ Qualified Enzymes: (Enzymes that digest completely in 5 minutes)

Acc65I	AciI	AclI	AflII	AgeI	AhdI
AluI	AlwI	AlwNI	ApaI	ApaLI	ApeKI
ApoI	AscI	AseI	AsiSI	AvaI	AvaII
AvrII	BamHI	BanII	BbvCI	BbvI	BciVI
BclI	BfuAI	BglI	BglII	BlpI	BmgBI
BsaAI	BsaBI	BsaXI	BseRI	BsgI	BsiEI
BsiHKAI	BsiWI	BslI	BsmAI	BsmFI	BsmI
BsoBI	Bsp1286I	BspEI	BsrBI	BsrDI	BsrFI
BsrI	BssHII	BstBI	BstEII	BstNI	BstUI
BstXI	BstZ17I	BtgI	BtsCI	BtsI	ClaI
CspCI	CviQI	DdeI	DpnI	DraI	DraIII
EagI	EcoNI	EcoO109I	EcoRI	EcoRV	Fnu4HI
FokI	FseI	HaeIII	HhaI	HindIII	HinfI
HinP1I	HpaII	HphI	HpyCH4IV	HpyCH4V	KpnI
MboI	MboII	MfeI	MluI	MlyI	MmeI
MnlI	MslI	MspA1I	MspI	NciI	NcoI
NdeI	NotI	NruI	NsiI	NspI	PacI
PaeR7I	PflFI	PflMI	PmeI	PmlI	PpuMI
PstI	PvuI	PvuII	SacI	SacII	SalI
SbfI	ScaI	ScrFI	SfiI	SfoI	SmaI
SnaBI	SpeI	SphI	SspI	Taq^αI	Tsp509I
TspMI	TspRI	XbaI	XhoI	XmnI

Complete Digestion in 15 Minutes:

AatII	AccI	AcuI	BaeI	BbsI	BccI
BceAI	BfaI	BfuCI	Bme1508I	BmrI	BpmI
BsaHI	BsaWI	BsmBI	BcpCNI	BspHI	BsrGI
BssKI	BstYI	Bsu36I	Cac8I	CviAII	CviKI-1
DpnII	DrdI	EarI	EcoP15I	FspI	HaeII
HgaI	HincII	HpaI	Hpy188I	MseI	MwoI
NgoMIV	NheI	NlaIII	PshAI	RsaI	SapI
StuI	StyI	StyD4I	SwaI	TfiI	TseI
Tth111I	XcmI	XmaI


- Recombinant Enzyme