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Home  >Technical Resource  > Restriction Endonucleases Time-Saver Qualified Enzymes

Time-Saver™ Qualified Enzymes

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  • Enzymes powerful enough to digest in 5 minutes
  • Pure enough for overnight incubation
  • No special formulation
  • No added expense
At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction-modification systems. This focus allows us to provide extremely pure enzymes at concentrations that deliver more flexibility for your experimental design.
Whether you are quickly screening large numbers of clones, or setting up overnight digests, you will benefit from the high quality of our enzymes. Typically, a restriction digest involves the incubation of 1 µl of enzyme with 1 µg of purified DNA in a final volume of 50 µl for 1 hour. However, to speed up the screening process, choose one of NEB’s enzymes that are Time-Saver™ qualified. These enzymes will digest 1 µg of DNA in 5 minutes using 1 µl of enzyme under recommended reaction conditions. Unlike other suppliers, there is no special formulation, change in concentration or need to buy more expensive new lines of enzymes to achieve digestion in 5 minutes. In fact, 59% of our enzymes will digest 1 µg of DNA in 5 minutes, while 83% will fully digest in 15 minutes (see table). That means >180 of our restriction enzymes have the power to get the job done fast.

Power and purity of Time-Saver™ qualified enzymes: 1 µl of SalI digests 1 µg of DNA in 5 minutes with no indication of nuclease contamination in longer digests or overnight (O/N) samples. Marker (M) is the 1 kb DNA Ladder (NEB #N3232).

Also, since all of our enzymes are rigorously tested for nuclease contamination, you can safely set up digests for long periods of time without any degradation of your sample. Only NEB can offer you enzymes with power and purity - the power to digest in 5 minutes and the purity to withstand overnight digestions with no loss of sample.

Efficiency on different substrates may vary.  Testing was done using the unit definition substrate (ie. Lambda DNA).  Certain supercoiled plasmids may require a reduction in the amount of plasmid digested. Further information can be found on pages 259-260 of the 2005/06 NEB catalogue.

  Time-Saver™ Qualified Enzymes:  (Enzymes that digest completely in 5 minutes)
Acc65I Recombinant Enzyme AciI   AclI Recombinant Enzyme AflII Recombinant Enzyme AgeI Recombinant Enzyme AhdI Recombinant Enzyme
AluI Recombinant Enzyme AlwI Recombinant Enzyme AlwNI   ApaI Recombinant Enzyme ApaLI Recombinant Enzyme ApeKI Recombinant Enzyme
ApoI Recombinant Enzyme AscI Recombinant Enzyme AseI Recombinant Enzyme AsiSI Recombinant Enzyme AvaI Recombinant Enzyme AvaII Recombinant Enzyme
AvrII Recombinant Enzyme BamHI Recombinant Enzyme BanII   BbvCI Recombinant Enzyme BbvI Recombinant Enzyme BciVI  
BclI Recombinant Enzyme BfuAI   BglI Recombinant Enzyme BglII Recombinant Enzyme BlpI Recombinant Enzyme BmgBI  
BsaAI Recombinant Enzyme BsaBI   BsaXI   BseRI Recombinant Enzyme BsgI   BsiEI  
BsiHKAI   BsiWI Recombinant Enzyme BslI Recombinant Enzyme BsmAI   BsmFI Recombinant Enzyme BsmI Recombinant Enzyme
BsoBI Recombinant Enzyme Bsp1286I   BspEI Recombinant Enzyme BsrBI   BsrDI   BsrFI Recombinant Enzyme
BsrI   BssHII Recombinant Enzyme BstBI   BstEII Recombinant Enzyme BstNI   BstUI  
BstXI   BstZ17I   BtgI   BtsCI Recombinant Enzyme BtsI Recombinant Enzyme ClaI Recombinant Enzyme
CspCI Recombinant Enzyme CviQI Recombinant Enzyme DdeI Recombinant Enzyme DpnI Recombinant Enzyme DraI Recombinant Enzyme DraIII Recombinant Enzyme
EagI Recombinant Enzyme EcoNI   EcoO109I Recombinant Enzyme EcoRI Recombinant Enzyme EcoRV Recombinant Enzyme Fnu4HI Recombinant Enzyme
FokI Recombinant Enzyme FseI Recombinant Enzyme HaeIII Recombinant Enzyme HhaI Recombinant Enzyme HindIII Recombinant Enzyme HinfI Recombinant Enzyme
HinP1I Recombinant Enzyme HpaII Recombinant Enzyme HphI Recombinant Enzyme HpyCH4IV Recombinant Enzyme HpyCH4V Recombinant Enzyme KpnI Recombinant Enzyme
MboI Recombinant Enzyme MboII Recombinant Enzyme MfeI Recombinant Enzyme MluI Recombinant Enzyme MlyI Recombinant Enzyme MmeI Recombinant Enzyme
MnlI Recombinant Enzyme MslI   MspA1I Recombinant Enzyme MspI Recombinant Enzyme NciI Recombinant Enzyme NcoI Recombinant Enzyme
NdeI Recombinant Enzyme NotI Recombinant Enzyme NruI Recombinant Enzyme NsiI Recombinant Enzyme NspI Recombinant Enzyme PacI Recombinant Enzyme
PaeR7I Recombinant Enzyme PflFI   PflMI Recombinant Enzyme PmeI Recombinant Enzyme PmlI   PpuMI Recombinant Enzyme
PstI Recombinant Enzyme PvuI   PvuII Recombinant Enzyme SacI Recombinant Enzyme SacII Recombinant Enzyme SalI Recombinant Enzyme
SbfI Recombinant Enzyme ScaI Recombinant Enzyme ScrFI   SfiI Recombinant Enzyme SfoI Recombinant Enzyme SmaI Recombinant Enzyme
SnaBI Recombinant Enzyme SpeI Recombinant Enzyme SphI Recombinant Enzyme SspI Recombinant Enzyme TaqαI Recombinant Enzyme Tsp509I Recombinant Enzyme
TspMI   TspRI Recombinant Enzyme XbaI Recombinant Enzyme XhoI Recombinant Enzyme XmnI Recombinant Enzyme
 
Complete Digestion in 15 Minutes:
AatII Recombinant Enzyme AccI Recombinant Enzyme AcuI Recombinant Enzyme BaeI   BbsI   BccI Recombinant Enzyme
BceAI   BfaI   BfuCI   Bme1508I   BmrI   BpmI  
BsaHI   BsaWI   BsmBI Recombinant Enzyme BcpCNI   BspHI Recombinant Enzyme BsrGI  
BssKI   BstYI Recombinant Enzyme Bsu36I   Cac8I   CviAII Recombinant Enzyme CviKI-1 Recombinant Enzyme
DpnII Recombinant Enzyme DrdI   EarI Recombinant Enzyme EcoP15I Recombinant Enzyme FspI Recombinant Enzyme HaeII Recombinant Enzyme
HgaI Recombinant Enzyme HincII Recombinant Enzyme HpaI Recombinant Enzyme Hpy188I Recombinant Enzyme MseI Recombinant Enzyme MwoI Recombinant Enzyme
NgoMIV Recombinant Enzyme NheI Recombinant Enzyme NlaIII Recombinant Enzyme PshAI Recombinant Enzyme RsaI Recombinant Enzyme SapI Recombinant Enzyme
StuI   StyI Recombinant Enzyme StyD4I Recombinant Enzyme SwaI Recombinant Enzyme TfiI Recombinant Enzyme TseI  
Tth111I Recombinant Enzyme XcmI Recombinant Enzyme XmaI Recombinant Enzyme            
 
Recombinant Enzyme - Recombinant Enzyme
                       




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