Restriction Enzyme Technical Data
A selection of the most popular pages from our Restriction Enzyme Technical Resource
Popular Links
NEBuffer Chart - activity of all NEB enzymes in NEBuffers 1-4 plus EcoRI Buffer
Setting up a Restriction Enzyme Digest - a good starting point or refresher
Cloning Guide - guidance on everything from preparation of insert and vector to ligation
Troubleshooting Guide - covers incomplete or no digestion, unexpected cleavage patterns and smearing of DNA on your gel
Activity in a PCR Mix - restriction enzyme activity in standard Taq Buffer, Thermopol Taq Buffer, Phusion HF Buffer and Crimson Taq Buffer
Cleavage Close to the End of DNA fragments - for planning how many bases to add after your restriction enzyme sites in PCR primers
Cleavage of Supercoiled DNA - some plasmids require up to 20 Units of enzyme to linearize. Check that you're using enough enzyme here
Average Fragment Size Generated by Endonuclease Cleavage - for species including S. cerevisiae, E. coli, C. elegans, and Drosophila
Enzyme Tools
NEBcutter - generate restriction maps of your own sequence, view gels, ORF summary and more . . .
Enzyme Finder - search for a restriction enzyme by category, recognition sequence, or name to find information on any NEB enzyme.
Double Digest Finder - buffer and reaction conditions for digest requiring two restriction enzymes
iPhone App
Download NEB Tools App for your iPhone - quick and easy access to the most requested restriction enzyme information allows you to plan your experiments from anywhere. Sort your results from Enzyme Finder so they make sense to you, then email them to your inbox or connect directly to www.neb.com. Determine buffer and reaction conditions for experiments requiring two restriction enzymes using the Double Digest Finder.
Full Restriction Endonucleases Technical Resource - a complete listing of all our restriction enzyme data