DyNAmo™ qPCR and qRT-PCR Kits

Superior Choice for Quantitative Analysis -- Licensed for PCR*

DyNAmo Kit Selection Table

Chemistry	SYBR Green (DyNAmo™ SYBR® Green qPCR Kits)				Probe (DyNAmo™ Probe qPCR Kits)
Template	DNA		RNA		DNA		RNA
Instrument	Block	Capillary	Block	Capillary	Block	Capillary	Block	Capillary
Kit	F-410S/L F-400RS/L F-400S/L	F-420S/L	F-430S/L	F-440S/L	F-450S/L	F-450S/L	F-460S/L	F-460S/L

DyNAmo products are developed and manufactured by Finnzymes Oy. and distributed by New England Biolabs (UK) Ltd.

DyNAmo qPCR Advantages	DyNAmo 2-step qRT-PCR Advantages
Specificity: Modified Hot Start Polymerase (in F-410, F-420 and F-450) minimizes primer-dimers and non-specifically amplification Convenience: 2X master mix and optimized protocols Broad dynamic range: up to 8 orders of magnitude from one copy of the target gene	Convenience: Optimized 2X mixes for reverse transcription followed by qPCR Versatility: Reliable results with mRNA, total RNA, viral RNA and in vitro transcribed RNA Broad Dynamic range: up to 1 µg of RNA

Ordering Information Click catalog number for specific product information

DyNAmo™ HS SYBR® Green qPCR Kit
F-410S	40 reactions of 50 µl volume
F-410L	200 reactions of 50 µl volume
DyNAmo™ SYBR® Green qPCR Kits
F-400S	40 reactions of 50 µl volume
F-400L	200 reactions of 50 µl volume
DyNAmo™ SYBR® Green qPCR Kits with ROX Passive Reference Dye
F-400RS	40 reactions of 50 µl volume
F-400RL	200 reactions of 50 µl volume
DyNAmo™ Capillary SYBR® Green qPCR Kit
F-420S	100 reactions of 20 µl volume
F-420L	500 reactions of 20 µl volume
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
F-430S	20 RT reactions of 20 µl volume + 40 qPCR reactions of 50 µl volume
F-430L	100 RT reactions of 20 µl volume + 200 qPCR reactions of 50 µl volume
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
F-440S	20 RT reactions of 20 µl volume + 100 qPCR reactions of 20 µl volume
F-440L	100 RT reactions of 20 µl volume + 500 qPCR reactions of 20 µl volume
DyNAmo™ Probe qPCR Kit
F-450S (available soon)	40 reactions of 50 µl volume
F-450L (available soon)	200 reactions of 50 µl volume
DyNAmo™ Probe 2-Step qRT-PCR Kit
F-460S (available soon)	20 RT reactions of 20 µl volume + 40 qPCR reactions of 50 µl volume
F-460L (available soon)	100 RT reactions of 20 µl volume + 200 qPCR reactions of 50 µl volume

DyNAmo products are developed and manufactured by Finnzymes Oy. and distributed by New England Biolabs (UK) Ltd

Engineered for qPCR

Finnzymes' DyNAmo™ qPCR Kits are a superior choice for quantitative real-time analysis. These kits offer excellent performance in detection and quantification of DNA and RNA sequences from various sources. DyNAmo qPCR Kit family contains optimized kits for both SYBR® Green and probe chemistries with various platforms. All DyNAmo qPCR Kits are provided as convenient 2x master mixes - only template and primers need to be added. Rox passive reference dye is included in the kits as a separate tube (not included in capillary kits F-420S/L and F-440S/L or block kit F-400S/L). All Finnzymes' DyNAmo qPCR Kits contain dUTP and therefore UNG (uracil-N-glycosylase) treatment can be used to minimize the effect of carry-over contamination. Use the table below to select the right kit for your purposes.

DyNAmo™ qPCR Kits

DyNAmo qPCR kits are a superior choice for quantitative real-time analysis. These kits offer unparalleled performance in detection and quantiﬁ cation of DNA sequences of interest from various sources. The performance of the DyNAmo kits is based on a uniquely engineered Thermus brockianus DNA Polymerase. The DyNAmo qPCR kit family contains optimized kits for both SYBR® Green and probe chemistries designed for block-based or capillary-based instruments. All DyNAmo qPCR kits are rovided as convenient 2X master mixes. Only template and primers need to be added.

DyNAmo™ Probe qPCR Kit

DyNAmo Probe qPCR Kit is designed for quantitative real-time PCR with probe detection. The kit is compatible with sequence specific probes, e.g. TaqMan® probes. The performance of the DyNAmo Probe qPCR Kit is based on a hot start Tbr DNA polymerase. DyNAmo Probe qPCR Kit is compatible with both blockbased and capillary-based instruments.

DyNAmo™ 2-Step qRT-PCR Kits

The DyNAmo qPCR kit family includes 2-Step qRT-PCR kits for SYBR Green and probe chemistries for use with both block-based and capillary-based instruments. The performance of the kits is based on an optimized master mix and a hot start version of a uniquely modiﬁed Thermus brockianus polymerase. The kits contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kits. Alternatively, the user can use gene speciﬁ c primers. The reverse transcriptase in DyNAmo 2-Step qRT-PCR kits is M-MuLV RNase H+, which provides high sensitivity to qPCR and facilitates the annealing of PCR primers to the cDNA by degrading the RNA template from the RNAcDNA hybrid before the PCR step. RNase inhibitor is mixed with the
RT-enzyme. The use of a 2-Step qRT-PCR protocol enables close control and monitoring of both cDNA synthesis and the qPCR step, resulting in a reliable RNA quantiﬁ cation. With 2-Step kits it is possible to detect multiple targets from one cDNA synthesis reaction.

DyNAmo™ SYBR® Green qPCR Kits

DyNAmo SYBR Green qPCR Kits are 2X master mixes designed for quantitative real-time PCR using SYBR Green I dye for detection. The performance of the DyNAmo SYBR Green qPCR Kits is based on a uniquely modified Thermus brockianus DNA polymerase and SYBR Green I fluorescent dye. Optimized qPCR buffer together with optimal SYBR Green I concentration enable the modified DNA polymerase to amplify target DNA reproducibly and with great sensitivity.

Advantages

Specificity: Modified hot start Thermus brockianus DNA polymerase (in F-410S/L and F-420S/L) minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers.
Sensitivity: Optimized master mix allows reproducible detection of low-copy number templates.
Broad dynamic range: Delivers a dynamic range of up to eight orders of magnitude starting even from one copy of the target gene.

A 335 bp PCR product was amplified from lambda DNA with DyNAmo HS SYBR Green qPCR Kit. A reaction volume of 20 µl was used with starting template copy number ranging from 5 to 50 000 000 copies. Reactions were set up on DNA Engine Opticon® System (Bio-Rad Laboratories).

DyNAmo™ HS SYBR® Green qPCR Kit (F-410S/L) is an optimized hot start kit for all block-based real-time instruments.
F-410S	40 reactions of 50 µl volume
F-410L	200 reactions of 50 µl volume
DyNAmo™ SYBR® Green qPCR Kits with ROX (F-400RS/L) or without ROX (F-400S/L) are optimized for block-based instruments.
F-400RS	40 reactions of 50 µl volume
F-400RL	200 reactions of 50 µl volume
DyNAmo™ Capillary SYBR® Green qPCR Kit (F-420S/L) is an optimized hot start kit for real-time instruments using capillary tubes.
F-420S	100 reactions of 20 µl volume
F-420L	500 reactions of 20 µl volume

DyNAmo™ SYBR® Green 2-Step qRT-PCR Kits

DyNAmo SYBR Green 2-Step qRT-PCR Kits enable reproducible detection and quantification of RNA with great sensitivity and specificity. The kits include all the necessary reagents for quantitative cDNA synthesis and SYBR Green qPCR. The RT-step in these kits is based on M-MuLV RNase H⁺, which provides higher sensitivity to qPCR than RNase H- reverse transcriptases. The performance of the qPCR step in DyNAmo SYBR Green 2-Step qRT-PCR Kit is based on F-410S/L kit and the performance of DyNAmo Capillary SYBR Green 2-Step qRT-PCR Kit is based on F-420S/L kit.

Advantages

Broad dynamic range: up to 1 µg of RNA.
Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA.
Flexible: two-step system, all priming options.
Optimized 2X mixes for both cDNA synthesis and qPCR.
Specific and sensitive: uniquely engineered Tbr DNA polymerase.
Convenient to use: 2X mixes and short protocols.

DyNAmo Capillary SYBR Green 2-Step qRT-PCR Kit was used to transcribe 0.2 fg - 0.02 ng bacteriophage MS2 RNA and a constant 100 ng amount of Human HeLa cell total RNA using random priming. The cDNA synthesis reactions were done in 20 µl volumes, from which 1/14 was transferred to qPCR reactions. A 113 bp fragment was amplified from cDNA with MS2 specific primers. Reactions were set up on the LightCycler™ System (Roche).

DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit (F-430S/L) is a two-step hot start kit including all reagents needed for cDNA synthesis and following qPCR. The kit is optimized for block-based real-time instruments.
F-430S	20 RT reactions of 20 µl volume + 40 qPCR reactions of 50 µl volume
F-430L	100 RT reactions of 20 µl volume + 200 qPCR reactions of 50 µl volume
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit (F-440S/L) is a two-step hot start kit including all reagents needed for cDNA synthesis and following qPCR. The kit is optimized for real-time instruments using capillary tubes.
F-440S	20 RT reactions of 20 µl volume + 100 qPCR reactions of 20 µl volume
F-440L	100 RT reactions of 20 µl volume + 500 qPCR reactions of 20 µl volume

DyNAmo™ Probe qPCR Kit

The DyNAmo Probe qPCR Kit is a 2X master mix designed for quantitative real-time PCR using both block-based and capillary-based instruments. The performance of the DyNAmo Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. The kit contains optimized PCR reagents in a master mix, and it can be used in qPCR with different sequence specific probes (e.g. TaqMan® probes).

Advantages

Specificity and sensitivity: Combines probe chemistry with optimized reagents.
Easy to use: 2X master mix and optimized protocols.
Broad dynamic range: Delivers a dynamic range of up to eight orders of magnitude.

Human papilloma virus genome was cloned into a plasmid. Dilution series from 10⁸ to 10 copies was prepared to be used as a standard DNA. A 76 bp fragment was amplified with F-450L DyNAmo Probe qPCR master mix and detected with a HEX-BHQ labeled TaqMan® probe. Reactions were setup on ABI 7500 Real Time PCR System (Applera Corporation). The slope of the standard curve was -3.363 and the correlation coefficient R² was 0.9997.

DyNAmo™ Probe qPCR Kit is an optimized hot start kit for both block-based and capillary-based real-time instruments.
F-450S	40 reactions of 50 µl volume
F-450L	200 reactions of 50 µl volume

DyNAmo™ Probe 2-Step qRT-PCR Kit

DyNAmo Probe 2-Step qRT-PCR Kit is a two-step hot start kit including all reagents needed for quantitative cDNA synthesis and qPCR. The kit is optimized for both block-based and capillary-based real-time instruments. The cDNA synthesis step in this kit is based on M-MuLV RNase H⁺, as in all DyNAmo 2-Step qRT-PCR Kits. The qPCR step is based on F-450S/L kit.

Advantages

Broad dynamic range: up to 1 µg of RNA.
Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA.
Flexible: two-step system, all priming options.
Optimized 2X mixes for both cDNA synthesis and qPCR.
Specific and sensitive: efficient Tbr DNA polymerase.
Convenient to use: 2X mixes and short protocols.

Human HeLa cell total RNA amounts varying from 10 pg to 1µg were reverse transcribed using oligo(dT) priming. The cDNA synthesis reactions were done in 20 µl volumes, from which 1/14 was transferred to qPCR reactions. A Calmodulin 1 fragment was amplified with cDNA specific primers and detected with an LNA probe (ProbeLibraryT, Exiqon). Reactions were done with DyNAmo Probe 2-Step qRT-PCR Kit and run with DNA Engine Opticon® system (Bio-Rad Laboratories).

DyNAmo™ Probe 2-Step qRT-PCR Kit is a two-step hot start kit including all reagents needed for cDNA synthesis and following qPCR. The kit is optimized for block-based and capillary-based real-time instruments.
F-460S	20 RT reactions of 20 µl volume + 40 qPCR reactions of 50 µl volume
F-460L	100 RT reactions of 20 µl volume + 200 qPCR reactions of 50 µl volume

DyNAmo is a trademark of Finnzymes Oy. SYBR is a registered trademark of Molecular Probes, Inc. DNA Engine Opticon is a registered trademark of Bio-Rad Laboratories, Inc. LightCycler is a trademark of a member of the Roche Group. TaqMan is a registered trademark of Roche Molecular Systems, Inc. ProbeLibrary is a trademark of Exiqon A/S.

* PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F.Hoffman-La Roche LTD, Roche Molecular Systems,Inc. and The Perkin-Elmer Corporation. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Perkin-Elmer or as purchased, i.e. an authorized thermal cycler.

Notice to purchaser: Limited license. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.

The use of TaqMan® fluorogenic probes in 5´ nuclease assays is covered by U. S. Patent Nos. 5,210,015 and 5,487,972, owned by Roche Molecular Systems, Inc, and by U. S. Patent No. 5,538,848, owned by The Applera Corporation. Purchase of the DyNAmo Probe qPCR Kits does not provide a license to use this patented technology. Further information on purchasing licenses to practice this technology may be obtained from Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404 or from Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, CA 94501, USA.

Use of DyNAmo Probe qPCR Kits may also require licenses from other third parties such as, but not limited to, the owners of hybridization probes and molecular beacons. Purchase of DyNAmo Probe qPCR Kits does not provide such licenses and further information on purchasing licenses to practice this technology must be obtained from the appropriate intellectual property owners of such technology.