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Home > Products > NEBNext® RNA Library Prep Reagents

NEBNext® RNA Library Preparation

 
 
 
 
NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.
 
Advantages of NEBNext Reagents for RNA Library Preparation:
  • Convenient formats – Enzymes, buffers and nucleotides are included, at appropriate concentrations and in appropriate volumes, and are available in set or master mix format. Large and small sizes are available.
  • Functional Validation – Each set is validated together by preparation of a library from an accepted RNA reference sample, that is sequenced using an Illumina GAIIx.
  • Stringent Quality Controls
  • Value Pricing
  • Novel Protocol for small RNA - Higher yield and substantially reduced adaptor dimer formation suitable for methylated and unmethylated small RNAs.

Applications of NEBNext Reagents for RNA Library Preparation:
  • mRNA library preparation for next generation sequencing
  • Small RNA library preparation for sequencing with Illumina and SOLiD
  • Expression library construction

Conversion of Purified mRNA to DNA Followed by Library Construction
 
RNA Sample Prep
 
Conversion of Purified Small RNA to DNA Followed by Library Construction
Small RNA Sample Prep

PRODUCT NEB # SIZE
COMPATIBLE
SEQUENCING
PLATFORM
NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® E7420S/L 24/96 reactions Illumina®
NEBNext® Ultra RNA Library Prep Kit for Illumina® E7530S/L 24/96 reactions Illumina®
NEBNext® mRNA Library Prep Reagent Set for Illumina® E6100S/L
10/50 Reactions
 Illumina®
NEBNext® mRNA Library Prep Master Mix Set for Illumina® E6110S/L
10/50 Reactions
 Illumina®
NEBNext® mRNA Second Strand Synthesis Module* E6111S/L
20/100 Reactions
 Multiple
NEBNext® mRNA Library Prep Reagent Set for 454™ E6115S/L
10/50 Reactions
 454™
NEBNext® mRNA Library Prep Master Mix Set for 454™ E6116S/L
10/50 Reactions
 454™
NEBNext® mRNA Library Prep Master Mix Set for SOLiD™ E6114S/L
10/50 Reactions
 SOLiD™
NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (1-12) E7300S/L 24/96 Reactions Illumina®
NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) E7330S/L 24/96 Reactions Illumina®
NEBNext® Multiplex Oligos for Illumina® (Index Primers 1-12) E7335S 24/96 Reactions Illumina®
NEBNext® Small RNA Library Prep Set for SOLiD™ E6160S/L
10/50 Reactions
 SOLiD™
NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) E7450S 32 Reactions SOLiD™
COMPANION PRODUCT
NEBNext® Second Strand Synthesis (dNTP-Free) Reaction Buffer B6117S 0.4 ml Multiple
 
* For applications requiring alternative concentrations of deoxynucleotides, NEBNext Second Strand Synthesis (dNTP-free) Reaction Buffer is available (NEB #B6117S).
 
 

HiSeq™ is a trademark of Illumina, Inc.
Illumina® is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Life Technologies.
454 is a trademark of Roche.