Acetyl-CoA Carboxylase 1 Antibody #4190
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| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M R | Endogenous | 265 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Acetyl-CoA Carboxylase 1 Antibody detects endogenous levels of total acetyl-CoA carboxylase 1 protein. Immunofluorescence data indicate that the antibody is more reactive to rodent than human proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with either SignalSilence® ACC1 siRNA or SignalSilence® ACC2 siRNA, using Acetyl-CoA Carboxylase 1 Antibody and β-Actin (13E5) Rabbit mAb #4970.
Western Blotting
Western blot analysis of extracts from various cell types using Acetyl-CoA Carboxylase 1 Antibody.
IF-IC
Confocal immunofluorescent analysis of HCC827 (left) and C2C12 cells (right) using Acetyl-CoA Carboxylase 1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).
- Ruderman, N.B. et al. (1999) Am. J. Physiol. 276, E1-E18.
- Ha, J. et al. (1994) J. Biol. Chem. 269, 22162-22168.
- Abu-Elheiga, L. et al. (2001) Science 291, 2613-2616.
- Levert, K.L. et al. (2002) J. Biol. Chem. 277, 16347-16350.
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.