Human Neurotrophin-4 (NT-4) #3887
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| MW | Source | Purity |
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| 22 | E. coli | >95% |
Description
Human NT-4 was prepared in E. coli and is supplied in a lyophilized form. A greater than 95% purity was determined by HPLC and SDS-PAGE.
Concentration and Specific Activity
10 μg lyophilized
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, transfected with TrkB and treated with 50 ng/ml and 100 ng/mlHuman Neurotrophin-4 (NT-4) for 5 minutes, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper), Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619 (middle) and a total TrkB antibody (lower).
Directions for Use
The working concentration for NT-4 generally ranges from 50-100 ng/ml.
Background
Neurotrophins are comprised of at least four family members including NGF, BDNF, NT-3 and NT-4 and all are known to influence growth, development, differentiation and survival of neurons (1). Proneurotrophins bind to p75NTR but not to the family of Trk receptor tyrosine kinases (Trk). Following maturation, NT-3 binds and activates TrkA, TrkB and TrkC, with TrkC almost exclusively binding to NT-3. Mature NT-4 binds to and activates TrkB (2). Trk receptors in turn activate three major signaling pathways: (a) Ras/MAPK, which promotes neuronal differentiation and neurite outgrowth, (b) PI3 Kinase/Akt, which promotes survival and growth of neurons, and (c) PLC-γ 1/PKC, which promotes synaptic plasticity (2).
Unlike knockout mice of other neurotrophins, NT-4-/- mice do not die early, suggesting that NT-4-dependent neurons are not critical for survival. However, the absence of NT-4 does result in sensory neuron loss (3,4). These mice also have deficits in long-term memory and long-lasting long-term potentiation (L-LTP) in the hippocampus but have normal short term-memory (5).
- Minichiello, L. and Klein, R. (1996) Genes Dev 10, 2849-58.
- Reichardt, L.F. (2006) Philos Trans R Soc Lond B Biol Sci 361, 1545-64.
- Conover, J.C. et al. (1995) Nature 375, 235-8.
- Liu, X. et al. (1995) Nature 375, 238-41.
- Xie, C.W. et al. (2000) Proc Natl Acad Sci USA 97, 8116-21.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products- 3897 Human Brain-Derived Neurotrophic Factor (BDNF)
- 3898 Human Neurotrophin-3 (NT-3)
- 4619 Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb
- 4621 Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb
- 2508 TrkA (14G6) Rabbit mAb
- 4603 TrkB (80E3) Rabbit mAb
- 2693 p75NTR Antibody
- 2046 NGF Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.