eIF2α (L57A5) Mouse mAb #2103
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| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P | H M R Mk | Endogenous | 38 | Mouse IgG2b |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
- 2103:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
eIF2α (L57A5) Mouse mAb detects endogenous levels of total eIF2α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified recombinant human eIF2α.
Western Blotting
Western blot analysis of extracts from various cell types using eIF2α (L57A5) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF2α (L57A5) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF2α (L57A5) Mouse mAb.
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
- Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
- De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
- Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
- Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
- Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.