4E-BP1 Antibody #9452
 |
|
|
|
|
|
|
|
|
|
|---|---|---|---|---|---|---|---|---|---|
|
Catalog # | |
Size & Concentration | | Price(£) | |
Qty | |
|
| |||||||||
| |||||||||
| |||||||||
| Please contact us for bulks/custom orders/drug discovery applications | |||||||||
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 15 to 20 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
4E-BP1 Antibody detects endogenous levels of total 4E-BP1, independent of phosphorylation.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Antibodies are purified by protein A affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using 4E-BP1 Antibody.
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
- Pause, A. et al. (1994) Nature 371, 762-767.
- Brunn, G.J. et al. (1997) Science 277, 99-101.
- Gingras, A.C. et al. (1998) Genes Dev. 12, 502-513.
- Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240-10247.
- Gingras, A.C. et al. (1999) Genes Dev. 13, 1422-1437.
- Rapisarda, A. et al. (2004) Topoisomerase I-mediated inhibition of hypoxia-inducible factor 1: mechanism and therapeutic implications. Cancer Res. 64, 1475-1482. Applications: Western Blotting
- Ohne, Y. et al. (2008) J Biol Chem 283, 31861-70. Applications: Western Blotting
- Hara, T. et al. (2008) J Cell Biol 181, 497-510. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products- 9644 4E-BP1 (53H11) Rabbit mAb
- 2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
- 9456 Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb
- 9455 Phospho-4E-BP1 (Thr70) Antibody
- 9459 Phospho-4E-BP1 (Thr37/46) Antibody
- 9451 Phospho-4E-BP1 (Ser65) Antibody
- 4923 Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
- 9955 4E-BP Antibody Sampler Kit
- 9918 Translational Control Antibody Sampler Kit
- 2845 4E-BP2 Antibody
- 9741 Phospho-eIF4E (Ser209) Antibody
- 9742 eIF4E Antibody
- 7216 PathScan® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Kit
- 1053 4E-BP1 Blocking Peptide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.