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| Human DNA (cytosine-5) Methyltransferase (Dnmt3A) Amino-terminal Ab | |
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| Download: | | MSDS PDF | |
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Description:
Dnmt3A is a de novo mammalian methyltransferase that is architecturally similar to Dnmt1 and Dnmt3B (1). The amino terminus (N-terminus) of human Dnmt3A contains various motifs that recruit transcriptional repressors (2). Dnmt3A cooperates with other DNA methyltransferases to maintain DNA methylation (3). Dnmt3A null embryos show multiple developmental defects including growth impairment and neural tube formation (4). Overexpression of DNA methyltransferases are observed in acute and chronic myelogenous leukemia cells (5). The apparent molecular weight for Dnmt3A is 101.5 kDa.
Sensitivity: Human DNA (cytosine-5) Methyltransferase (Dnmt3A) amino-terminal antibody detects less than 20 ng of Dnmt3A in the cell extract using anti-rabbit Phototope-HRP Western Detection Kit (available from Cell Signaling Technology).
Figure 1: Western analysis of cell extracts using Human DNA (cytosine-5) Methyltransferase 3A Amino-Terminal Antibody; Lane 1: HeLa cells, Lane 2: A549 cells, Lane 3: A673 cells, Lane 4: 3T3 cells, Lane 5: C6 cells.
Specificity/Cross-reactivity: human, rat, mouse and monkey
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| Antibody Application | Recommended Dilution | Sensitivity | Detection Method | |||||
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| Western Blot | 1:2000 | 20 ng | Western Blot | |||||
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Storage Conditions
Storage Conditions:
10 mM Sodium HEPES
150 mM NaCl
100 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C
Storage Temperature:
-20°C
References
- Okano, M. et al. (1998) Nature Genet., 19, 219-220.
- Pradhan, S. et al. (2003) Clinical Immunology, 109, 6-16.
- Kim, G. D. et al. (2002) EMBO J, 21, 4183-4195.
- Okano, M. et al. (1999) Cell, 99, 247-257.
- Mizuno, S. et al (2001) Blood, 97, 1172-1179.