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| Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Ab | |
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| Download: | | MSDS PDF | |
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Description:
Amino terminal (N-terminal) of human Dnmt1 has been shown to bind proliferating cell nuclear antigen (PCNA) and histone deactylase (1,2). Deletion of this region stimulates DNA methylation (3). In neoplastic transformed cells methylation pattern is abnormal, leading to the speculation that Dnmt1 may be involved in such processes (4).
Sensitivity:
Human DNA (cytosine-5) Methyltransferase (Dnmt1) Amino-terminal Antibody detects less than 10 ng of Dnmt1 in the cell extract using anti-rabbit Phototope-HRP Western Detection Kit (available from Cell Signaling Technology).
Dnmt1 Amino-terminal Antibody:
Polyclonal antibodies are produced by immunizing rabbit with a KLH coupled synthetic peptide corresponding to residues in the N-terminal region of human Dnmt1. The antibodies are purified in a protein A column.
Figure 1: Western analysis of cell extracts; Lane 1: Sf9 cells, Lane 2: Human DNA (cytosine-5) Methyltransferase expressing Sf9 cells, Lane 3: 293 cells, Lane 4: Hela cells, Lane 5: Jurkat cells, Lane 6: COS cells, Lane 7: Mouse DNA (cytosine-5) Methyltransferase expressing Sf9 cells, using Human DNA (cytosine-5) Methyltransferase Amino-terminal Antibody.
Specificity/Cross-reactivity: human, monkey
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| Antibody Application | Recommended Dilution | Sensitivity | Detection Method | |||||
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| Western Blot | 1:2000 | 10 ng | Western Blot | |||||
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Storage Conditions
Storage Conditions:
10 mM HEPES
150 mM NaCl
100 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C
Storage Temperature:
-20°C
FAQs
- What dilution should be used with Dnmt1 Amino-terminal Ab?
- How much protein should be run on the gel for detection with Dnmt1 Amino-terminal Ab?
- How much of NEB Dnmt1 should be used as a control when using Dnmt1 Amino-terminal Ab?
- What is the species cross reactivity of Dnmt1 Amino-terminal Ab?
- Can Dnmt1 Amino-terminal Ab be used to detect mouse and rat?
References
- Chuang, L.S. et al. (1997) Science, 277, 1996-2000.
- Fuks, F. et al. (2000) Nature Genet., 24, 88-91.
- Bestor, T.H. (1992) EMBO J., 11, 2611-2617.
- Schmutte, C. et al. (1998) Biol. Chem., 379, 377-388.
Companion Products
Human DNA (cytosine-5) Methyltransferase (Dnmt1)