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| Download: | | MSDS PDF | |
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- Extensive set of restriction sites in polylinker, many with unique 4-base overhangs
- Blue/white section
- Opposing T7promoters for making RNA transcripts in either direction or double-stranded RNA using the HiScribe™ T7 In Vitro Transcription Kit (NEB #E2030)
LITMUS 28i is a multi-purpose cloning/in vitro transcription phagemid vector. The molecule is a small, double-stranded circle 2,823 base pairs in length (molecular weight�=1.8 x 106 daltons).
Source:
LITMUS 28i is isolated from E. coli ER2738 by a standard plasmid purification procedure.
Advantages:
- Small (< 3 kb), high copy number.
- Ampicillin resistance.
- Single-stranded (M13) DNA replication origin.
- Compatible with pUC/M13 sequencing primers as well as LITMUS sequencing primers.
500 μg/ml
Storage Conditions
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:
- Appropriate strains of E. coli containing the LITMUS 28i plasmid form blue colonies on X-gal plates; when a fragment is inserted in the polylinker, the colonies are white. Blue/white selection is best achieved by plating on a rich media (e.g. LB) supplemented with X-gal (40��g/ml) and IPTG (0.1 mM).
FAQs
- How homogeneous is the DNA/ how much is supercoiled?
- Are the plasmids cesium purified?
- What primers should I use to sequence an insert (pUC19, pNEB193, LITMUS)?
Enzymes in parentheses ( ) leave 3' extension and should not be used for template linearization.
Companion Products
HiScribe™ T7 In Vitro Transcription Kit
LITMUS 28/38 Forward Sequencing Primer (21-mer)
LITMUS 28/38 Reverse Sequencing Primer (21-mer)
LITMUS 38i Vector
Legal
Patents:
New England Biolabs, Inc.: U.S. 5,691,140
Research Use Assurance:
The Buyer/User has a non-exclusive license to use this vector for RESEARCH PURPOSES ONLY.* Commercial use of the LITMUS Vectors may require a license from New England Biolabs.
*Use of this vector for RNA interference may require a license�from the Carnegie Institution of Washington. For further information, contact the Director of Administration and Finance at the Carnegie Institute of Washington at 1530 P Street, N.W., Washington�D.C., 20005-1910. Tel: 202-939-1118.