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| Download: | | MSDS PDF | |
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Description:
Deoxynucleotide Solution Mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP.
Reagents Supplied:
10 mM Deoxynucleotide Solution Mix 5 x 0.8 ml (large); 4 x 0.2 ml (small)
Example of Use: For a reaction which calls for 200 µM of each dNTP, add 2 µl of this premixed nucleotide solution per 100 µl reaction.
Diluent Compatibility: Can be diluted using sterile distilled water, preferably Milli-Q™ water or can be diluted using sterile TE (10 mM Tris-HCl, 1 mM EDTA (pH 7.5).
Reaction & Storage Conditions
Concentration:
10 mM each nt
Storage Conditions:
10 mM Milli-Q water
pH 7.5 @ 25°C
Storage Temperature:
-20°C
Notes
General notes:
- Storing nucleotide triphosphates in solutions containing magnesium promotes triphosphate degradation.
- For use with any DNA Polymerases from NEB.
- Milli-Q? is a trademark of Millipore Corporation.
FAQs
- What is supplied with the Deoxynucleotide Solution Mix?
- What is in the Deoxynucleotide Solution Mix?
- How stable are the nucleotides?
- What is the concentration of Deoxynucleotide Solution Mix?
- What polymerases can the Deoxynucleotide Solution Mix be used with?
- How does this product differ from the Deoxynucleotide Solutions Set (NEB# N0446)?
- If a final concentration of 200 μM each dNTP is called for, how much of the mix should be added to a 100 μl reaction?
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
The purity of each deoxynucleotide is ≥ 99% as determined by HPLC analysis.
0.5 kb, 2 kb and 5 kb Lambda PCR Assay:
25 cycles of PCR amplification of 1 ng Lambda DNA with 5 units of Taq DNA Polymerase in the presence of 200 µM Deoxynucleotide Solution Mix, 0.5 µM primers and 1X ThermoPol Buffer results in the amplification of the specific 0.5 kb, 2 kb and 5 kb products as determined by agarose gel electrophoresis.
Phosphatase Activity Assay (pNPP Colorimetric Assay):
A protein phosphatase buffer solution containing 2 mM Deoxynucleotide Solution Mix and 100 µM p-nitrophenol phosphate, incubated for 4 hours at 37°C, yields no detectable phosphatase activity as determined by spectrophotometric analysis of released p-nitrophenylene anion at 405 nm.
Non-specific Nuclease Assay:
A 50 µl reaction in 1X NEBuffer 2 containing 1 µg of T3 DNA or HindIII digested Lambda DNA and a minimum of 10 µl of Deoxynucleotide Solution Mix incubated for 16 hours at 37°C results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.
Companion Products
Deoxynucleotide Solution Set