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- Licensed for PCR*
- Uncomparable Sensitivity - reproducible detection of low-copy number templates
- Broad Dynamic Range - up to eight orders of magnitude starting even from a single copy of the target gene
DyNAmo™ SYBR® Green qPCR Kit is a ready-to-use 2X master mix for sensitive, quantitative real-time PCR. The master mix contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA binding domain that lends physical stability to the polymerase-DNA complex. If a hot start polymerase is preferred, the DyNAmo HS SYBR Green qPCR Kit is the recommended choice.
In DyNAmo SYBR Green qPCR Kit with ROX Passive Reference Dye the ROX Passive Reference Dye is supplied with the kit in a separate tube. This kit is recommended for instruments that require ROX normalization.
Sensitivity, reproducibility and speed:
Amplification curves below show clearly that lower copy number detection and earlier C(t)-values are obtained with the DyNAmo SYBR Green qPCR Kit. Signal level is also considerably higher and reaction times are substantially reduced when using the DyNAmo SYBR Green qPCR Kit.
Request a qPCR booklet
Figures 1a and 2a: Amplification plot showing the linearity of the 10-fold dilutions of template as cycle numbers vs. fluorescence. Comparison of Finnzymes DyNAmo SYBR Green qPCR Kit (1) and a qPCR kit from a leading supplier (2). A 221 bp PCR product was amplified from a plasmid containing a fragment of a human gene. Reactions were set up on the DNA Engine Opticon system (MJ Research, Inc) according to suppliers instructions. Reaction volumes of 20 �l were used with starting template copy number ranging from 1 to 1,000,000 copies. Figures 1b,2b: Standard curves generated from C(t) values. The curves can be used for measuring unknown amounts of sample target (the same target as used in standards).
Advantages:
- Sensitivity: Optimized 2X master mix allows reproducible detection of low-copy number templates.
- Speed: More efficient amplification coupled with increased polymerase processivity results in earlier C(t)s and shorter overall qPCR reaction times. (See figures 1 and 2) (Threshold cycle, C(t), is the cycle at which the fluorescence reaches the threshold level. The threshold level is set manually or calculated automatically.)
- Convenience: 2X master mix, one tube only. In F-400RS/L ROX is provided in a separate vial.
- Broad dynamic range: DyNAmo SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude.
Master Mix (2X)
ROX Passive Reference Dye (50X)
Notes
General notes:
- Both DyNAmo SYBR Green qPCR kits are shipped in gel ice.
- Upon arrival, store all kit components at 4°C or -20°C.
- When stored and handled properly the DyNAmo SYBR Green qPCR kits are stable for six months in 4°C or one year in -20°C from the date of packaging.
FAQs
- What are the advantages to using DyNAmo™ SYBR® Green qPCR kits?
- What applications is DyNAmo™ SYBR® Green qPCR Kit recommended for?
- What is included in the DyNAmo™ SYBR® Green qPCR Kit?
- Do I need the ROX™ passive reference dye when I use DyNAmo™ SYBR® Green qPCR Kit?
- What is the difference between DyNAmo™ SYBR® Green qPCR kits (#F-400/F-400R/F-420) and DyNAmo™ HS SYBR® Green qPCR Kit (#F-410)?
- What is the enzyme in DyNAmo™ qPCR kits?
- I have no product after amplification using a DyNAmo™ qPCR kit.
- Can I clone the PCR product after amplifying with DyNAmo™ qPCR Kits?
- Does the enzyme in the DyNAmo™ qPCR kits add 3' A to the PCR product to make it compatible with TA cloning?
- What should I do if there are non-specific products that are not primer dimers in my no template control when using a DyNAmo™ qPCR kit?
- What should I do if there are bubbles in my qPCR reactions before cycling using a DyNAmo™ qPCR kit?
- Does the polymerase in DyNAmo™ qPCR kits have 5' → 3' exonuclease activity?
- Does the polymerase in DyNAmo™ qPCR kits have proofreading capabilities (3' → 5' exonuclease activity)?
- Is the enzyme in my DyNAmo™ qPCR kit a hot-start polymerase?
- At what temperature should the DyNAmo™ kit be stored?
- I have non-specific products after amplification using a DyNAmo™ qPCR kit.
- Are Finnzymes' DNA Polymerases licensed for PCR?
Protocols
Protocols for DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye
Companion Products
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ Capillary SYBR® Green qPCR Kit
DyNAmo™ cDNA Synthesis Kit
DyNAmo™ ColorFlash SYBR® Green qPCR Kit
DyNAmo™ Flash Probe qPCR Kit
DyNAmo™ Flash SYBR® Green qPCR Kit
DyNAmo™ HS SYBR® Green qPCR Kit
DyNAmo™ Probe qPCR Kit
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green qPCR Kit
Legal
Licenses/Patents/Disclaimers:
* PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. SYBR® Green I stain is covered by patents belonging to Molecular Probes and is provided under license from Molecular Probes, Inc. for use only in combination with real-time PCR.