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- Specificity — hot start polymerase minimizes primer dimer formation and non-specific products
- Sensitivity — reproducible detection of low copy number templates
- Broad Dynamic Range — excellent performance across 8 orders of magnitude
DyNAmo™ Probe qPCR Kit is a ready-to-use 2X master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA, PCR primers and probe need to be added by the user. The 2X master mix includes a hot start Thermus brockianus DNA polymerase.
ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.
DyNAmo Probe qPCR Kit is compatible with all block-based and capillary-based real-time qPCR instruments.
Request a qPCR booklet
Human Papilloma Virus genome was cloned into a plasmid. Dilution series from 108 to 10 copies was prepared to be used as a standard DNA. A 76 bp fragment was amplified with F-450L DyNAmo Probe qPCR Kit and detected with a HEX-BHQ labeled TaqMan® Probe. Reactions were setup on ABI 7500 Real Time PCR System (Applera Corporation, Inc.). The slope of the standard curve was -3.363 and the correlation coefficient R2 was 0.9997.
Advantages:
- Specificity and sensitivity: Combines probe chemistry with optimized reagents.
- Easy to use: 2X master mix and optimized protocols.
- Broad dynamic range: Delivers a dynamic range of up to eight orders of magnitude.
Master Mix (2X)
ROX Passive Reference Dye (50X)
Notes
General notes:
- The DyNAmo Probe qPCR Kit is shipped in gel ice.
- Upon arrival store all kit components at -20°C.
- The DyNAmo Probe qPCR Kit is stable for six months from the date of packaging when stored and handled properly.
- After use, the leftover thawed 2X master mix can be refrozen and stored at -20°C.
FAQs
Protocols
Protocols for DyNAmo™ Probe qPCR Kit
Companion Products
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ Capillary SYBR® Green qPCR Kit
DyNAmo™ cDNA Synthesis Kit
DyNAmo™ ColorFlash Probe qPCR Kit
DyNAmo™ ColorFlash SYBR® Green qPCR Kit
DyNAmo™ Flash Probe qPCR Kit
DyNAmo™ HS SYBR® Green qPCR Kit
DyNAmo™ Probe 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green qPCR Kit
Legal
Licenses/Patents/Disclaimers:
PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. Use of DyNAmo Probe qPCR Kits may also require licenses from other third parties such as, but not limited to, the owners of hybridization probes and molecular beacons. Purchase of DyNAmo Probe qPCR Kits does not provide such licenses and further information on purchasing licenses to practice this technology must be obtained from the appropriate intellectual property owners of such technology.