Substrate Selection Chart

Fluorogenic substrates for all of the tags can be divided into two broad categories: those which are permeable to the cell membrane (used for intracellular labeling) and those which are non-permeable (and are used for cell surface labeling).

FUSION	TAG	LABELING
SNAP - Tag	APPLICATIONS							Excitation	Emission	Catalog#
	Intracellular Imaging	local- ization	in-vitro analysis	surface labeling	fixed perme- ablized	SDS- PAGE	west- ern
	SNAP-Cell 430	X						421	444,484	S9109S
	SNAP-Cell 505		X					504	532	S9103S
	SNAP-Cell Oregon Green									S9104S
	SNAP-Cell Fluorescein	X						500	532	S9107S
	SNAP-Cell TMR-Star	X						554	580	S9105S
	Cell Surface Labeling
	SNAP-Surface 425		X					438	489	S9126S
	SNAP-Surface Alexa Fluor® 488		X	X				496	520	S9129S
	SNAP-Surface 488			X	X			506	526	S9124S
	SNAP-Surface 532		X		X			536	554	S9131S
	SNAP-Surface Alexa Fluor® 546		X	X	X			558	574	S9132S
	SNAP-Surface 549		X	X	X			554	568	S9112S
	SNAP-Surface 600		X					606	626	S9134S
	SNAP-Surface 632		X					637	657	S9135S
	SNAP-Surface Alexa Fluor® 647		X	X	X			652	670	S9136S
	SNAP-Surface 647			X	X	X		660	673	S9137S
	SNAP-Surface 682		X					693	708	S9139S
	SNAP-Surface 732		X					732	747	S9140S
	SNAP-Surface 747		X					752	763	S9141S
	SNAP-Surface 782		X					784	789	S9142S
	SNAP-Vista Blue						X	390	470	S9146S
	SNAP-Vista Green						X	500	524	S9147
	SNAP-Biotin		X	X	X					S9110S

FUSION	TAG	LABELING
CLIP - Tag	APPLICATIONS							Excitation	Emission	Catalog#
	Intracellular Imaging	local- ization	in-vitro analysis	surface labeling	fixed perme- ablized	SDS- PAGE	west- ern
	CLIP-Cell 360	X						357	437	S9215S
	CLIP-Cell 430	X						421	444,484	S9216S
	CLIP-Cell 505		X					504	532	S9217S
	CLIP-Cell Fluorescein	X						500	532	S9218S
	CLIP-Cell TMR-Star	X						554	580	S9219S
	Cell Surface Labeling
	CLIP-Surface 488			X	X			506	526	S9232S
	CLIP-Surface 547		X	X	X			554	568	S9233S
	CLIP-Surface 647			X	X	X		660	673	S9234S
	CLIP-Vista Green						X	500, UV	524	S9235S
	CLIP-Biotin		X	X	X					S9221S

ENZYMATIC	TAG	LABELING
ACP/MCP Tag	APPLICATIONS						Excitation	Emission	Catalog#
	Cell Surface Labeling	local- ization	in-vitro analysis	surface labeling	fixed perme- ablized	SDS- PAGE
	CoA 488			X	X		506	526	S9348S
	CoA 547		X	X	X		554	568	S9349S
	CoA 647			X	X	X	660	673	S9350S
	CoA Biotin								S9351S