Properties of DNA Repair Enzymes

Enzyme	Substrate	Cleavage site	Termini created from cleavage
Enzyme	Substrate	Cleavage site	5'-terminus	3'-terminus
APE 1	AP sites	1st phosphodiester bond 5' to the lesion	dR5P	OH
Endo III	Oxidized Pyrimidines, AP sites	1st phosphodiester bond 3' to the lesion	P	PA
Endo IV	AP sites	1st phosphodiester bond 5' to the lesion	dR5P	OH
Endo V	Deoxyinosine, mismatches, hairpin/unpaired loop, flaps,Pseudo Y structures	2nd phosphodiester bond 3' to the lesion	P	OH
Endo VIII	Oxidized Pyrimidines, AP sites	1st phosphodiester bond both 5' and 3' to the lesion	P	P
Fpg	Oxidized Purines, AP sites	1st phosphodiester bond both 5' and 3' to the lesion	P	P
hOGG1	Oxidized Purines, AP sites	1st phosphodiester bond 3' to the lesion	P	PA
T7 Endo I	Cruciform, Holiday junctions, mismatches, heteroduplexes	1st, 2nd or 3rd phosphodiester bond 5' to the mismatch	P	OH
UDG	Deoxyuridine	N-glycosylic bond	AP site (no break)

Table Legend:

AP:	apurinic/apyrimidinic sites
P:	phosphate
OH:	hydroxyl
dR5P:	deoxyribose-5'-phosphate
PA:	3'-phospho-a, b-unsaturated aldehyde