PCR Reagents from NEB

NEB offers a wide range of DNA polymerases and through our commitment to research, ensures the development of innovative and high quality tools for PCR related applications. In addition, our collaboration with Finnyzmes Oy brings an unsurpassed offering of state-of-the-art products for PCR and qPCR combined with the service and quality for which NEB is known. Several factors influence the choice of an optimal enzyme for a specific PCR application. The following chart highlights the most significant properties to be considered when choosing a DNA polymerase for PCR.

	Fidelity vs Taq	Optimal Extn Range	Difficult Templates	Resulting DNA ends	3´→ 5´ exo	PCR Kit /Master Mix Available	Units /50µ Rxns	Advantages
Crimson Taq DNA Polymerase	1X	≤ 3kb	-	3'A	No	No/No	1.5-2.5	Taq with a colored buffer–load PCR product directly on a gel to track migration
Phire Hot Start DNA Polymerase(1)	2X	≤7.5kb	x	Blunt	Yes	No/No	1.0	Quick hot start (no reactivation step) High Speed (3X faster than Hot Start Taq) High Yield
Phusion High-Fidelity DNA Polymerase(1)	50X	≤20kb	xxx	Blunt	Yes	Yes/Yes	0.5-1.0	Extreme fidelity (50X greater than Taq) High speed (5X faster than Pfu) Robust reactions High yield Choice of formats and reaction buffers
Phusion Hot Start High-Fidelity DNA Polymerase(1)	50X	≤20kb	xxx	Blunt	Yes	No/No	0.5-1.0	Extreme specificity Extreme fidelity (50X greater than Taq) High speed (5X faster than Pfu) Robust reactions High yield
Deep Vent DNA Polymerase	5X	≤6kb	xx	Blunt	Yes	No/No	0.2-1.0	High-fidelity (5X greater than Taq) Extremely high thermostability (half-life of 23 hr at 95°C) Ideal for GC-rich or looped sequences
Vent DNA Polymerase	5X	≤6kb	x	Blunt	Yes	No/No	0.2-1.0	High-fidelity (5X greater than Taq) High thermostability (half-life of 6.7 hr at 95°C)
DyNAzyme EXT DNA Polymerase(1)	2X	≤40kb	xxxx	3´A & Blunt	Yes	Yes/No	0.5-2.0	Amplifies up to 40 kb Ideal for GC-rich or looped sequences Robust reactions with standard templates
DyNAzyme II Hot Start DNA Polymerase(1)	1X	≤3kb	-	3´A	No	No/No	0.75-2.0	Minimizes non-specific amplification Room temperature reaction set up Able to incorporate dUTP, dITP and fluorescently-labeled nucleotides
Taq DNA Polymerase	1X	≤3kb	-	3´A	No	Yes/Yes	0.5-2.0	Industry standard for routine PCR Choice of formats and reaction buffers to accomodate a variety of PCR applications Tolerates a wide range of templates Incorporates dUTP, dITP and flourescently-labeled nucleotides
LongAmp Taq DNA Polymerase	6X	≤40kb	xxxx	3´A & Blunt	Yes	Yes/Yes	5	Amplifies up to 40 kb from lambda DNA and up to 30 kb from human genomic DNA Tolerates a wide ramge of templates Robust reactions

(1) Manufactured by Finnzymes Oy. distributed by New England Biolabs, Inc.

Taq DNA Polymerase is the industry standard for routine PCR, known for robust and reliable reactions. NEB provides high quality, recombinant Taq at exceptional value in terms of cost per unit. For your convenience, this versatile enzyme is now available in a variety of formats:

Multiplex PCR 5X Master Mix	For 2 or more products in a single reaction
Crimson Taq DNA Polymerase	Load PCR product directly on a gel
Taq PCR Kit	Everything you need to perform 200 PCR reactions
Taq PCR Kit with Controls	Also includes 30 control reactions
Taq 2X Master Mix Taq 5X Master Mix	Just add template and primers
Quick-Load Taq 2X Master Mix	Also includes dyes for tracking
Taq DNA Polymerase with ThermoPol Buffer	Uniquely formulated buffer for high product yields
Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer	Additional Mg+2 control
Taq DNA Polymerase with Standard Taq Buffer	Detergent free buffer for high throughput applications
Taq DNA Polymerase with Standard Taq (Mg-free) Buffer	Additional Mg+2 control
Crimson Taq DNA Polymerase	Buffer contains density reagent for direct gel loading
Crimson Taq DNA Polymerase with Mg-Free Buffer	Mg-free Buffer contains density reagent for direct gel loading