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Characteristics of Thermophilic DNA Polymerases
| Vent | Vent (exo-) |
Deep Vent |
Deep Vent (exo-) |
Taq | 9°Nm | Phusion | DyNAzyme EXT |
DyNAzyme
II Hot Start |
|
| Exonuclease Activities | |||||||||
3'—>5'
proofreading |
yes | no | yes | no | no | 1-5% | yes | yes | no |
| 5'—>3'
exonuclease activity |
no | no | no | no | yes | no | no | yes | yes |
| Thermal Stability | |||||||||
| Half
life at 95°C (hours) |
6.7 | 6.7 | 23 | 23 | 0.9 | 6.7 | >6 at 96°C | 3.5 at 96°C | 0.9 |
| Half
life at 100°C (hours) |
1.8 | 1.8 | 8 | 8 | <0.1 | 1.8 | 2 at 98°C | <0.1 | |
| Primer Extension Characteristics | |||||||||
| Units/100
µl Primer Extension Rxn |
1-2 units | 2-4 units | 1-2 units | 2-4 units | 2-5 units | 1-2 units | 1-2 units | 1-4 units | 1-4 units |
| Error
Rate (x10-6 base insertions) |
57 | 190 | ~20 | ~190 | 285 | in prograss | .44 | ||
| Resulting DNA Ends | blunt ends | 3´ A | blunt ends | 3´ A | 3´ A | 3´ A blunt |
blunt | 3´ A blunt |
3´ A |
| Strand Displacement | yes; temp. dependent |
yes; temp. dependent |
yes; temp. dependent |
yes; temp. dependent |
no |
yes |
no | yes; temp. dependent |
no |
| Longest
Primer Extension to Date |
13.2 kb | 15.0 kb | 14.0 kb | 15.0 kb | 8.1 kb | 14.0 kb | 37 kb | 40 kb | 3 kb |