Activity of Thermophilic Enzymes at 37°c

Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

Enzyme	Optimal Temperature	% Activity at 37°C
Apa I	25°C	100*
ApeK I	75°C	<10
Apo I	50°C	50
Bae I	25°C	20
Bcl I	50°C	50
BfuA I	50°C	50
BpuE I	25°C	20
Bsa I	50°C	10
BsaB I	60°C	20
BsaJ I	60°C	20
BsaW I	60°C	20
BsiE I	60°C	30
BsiHKA I	65°C	<10
BsiW I	55°C	50
Bsl I	55°C	30
Bsm I	65°C	20
BsmA I	55°C	30
BsmB I	55°C	20
BsmF I	65°C	50
BspCN I	25°C	30
Bsr I	65°C	20
BsrD I	65°C	30
BssH II	50°C	75
BssK I	60°C	10
BstAP I	60°C	50
BstB I	65°C	10
BstE II	60°C	50
BstF5 I	65°C	20
BstN I	60°C	30
BstU I	60°C	20
BstX I	55°C	50
BstY I	60°C	30
BtgZ I	60°C	75
CviA II	25°C	20
Fat I	55°C	20
Fau I	55°C	20
Mwo I	60°C	10
N.BstNB I	55°C	<10
Nb.Bsm I	65°C	-
Pho I	75°C	<10
PI-Psp I	65°C	<10
PspG I	75°C	10
Sfi I	50°C	10
Sma I	25°C	50
Sml I	55°C	<10
Swa I	25°C	50
Taq I	65°C	10
Tfi I	65°C	10
Tli I	75°C	<10
Tse I	65°C	20
Tsp45 I	65°C	10
Tsp509 I	65°C	<10
TspR I	65°C	10
Tth111 I	65°C	10

* Apa I has 100% avtivity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.