Alphabetical List of Sequences

All restriction endonuclease recognition specificities available from New England Biolabs are listed below. For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. For example, CCTC(7/6) and (6/7)GAGG both represent an Mnl I site.

All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/".

Numbers in parentheses indicate point of cleaveage for non-palindromic enzymes.

For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´

Recognition Sequence	Enzymes
AA/CGTT	Acl I
A/AGCTT	Hind III
AAT/ATT	Ssp I
/AATT	Tsp509 I
A/CATGT	Pci I
A/CCGGT	Age I
ACCTGC(4/8)	BfuA I
ACCTGC(4/8)	BspM I
A/CCWGGT	SexA I
A/CGCGT	Mlu I
ACGGC(12/14)	BceA I
A/CGT	HpyCH4 IV
ACN/GT	HpyCH4 III
(10/15)ACNNNNGTAYC(12/7)	Bae I
(9/12)ACNNNNNCTCC(10/7)	BsaX I
A/CRYGT	Afl III
A/CTAGT	Spe I
ACTGG(1/-1)	Bsr I
ACTGGG(5/4)	Bmr I
A/GATCT	Bgl II
AGC/GCT	Afe I
AG/CT	Alu I
AGG/CCT	Stu I
AGT/ACT	Sca I
AT/CGAT	BspD I
AT/CGAT	Cla I
ATG/CAT	BfrB I
ATGCA/T	Nsi I
AT/TAAT	Ase I
ATTT/AAAT	Swa I
(11/13)CAANNNNNGTGG(12/10)	CspC I
C/AATTG	Mfe I
CACGAG(-5/-1)	BssS I
CACGTC(-3/-3)	BmgB I
CAC/GTG	Pml I
CACNNN/GTG	Dra III
CACNN/NNGTG	Ale I
(0/2)CACTGC	Bts I
CAGCAG(25/27)	EcoP15 I
CAG/CTG	Pvu II
CAGNNN/CTG	AlwN I
CASTGNN/	TspR I
CA/TATG	Nde I
(0/2)CATCC	BstF5 I
(13/9)CATCC	Fok I
(14/10)CATCGC	BtgZ I
C/ATG	CviA II
/CATG	Fat I
CATG/	Nla III
(0/2)CATTGC	BsrD I
CAYNN/NNRTG	Msl I
(10/12)CCACNNNNNTTG	CspC I
(-1/1)CCAGT	Bsr I
CCANNNNN/NNNNTGG	Xcm I
CCANNNNN/NTGG	BstX I
CCANNNN/NTGG	PflM I
CCATC(4/5)	Bcc I
C/CATGG	Nco I
CCCAGC(-5/-1)	BseY I
(4/5)CCCAGT	Bmr I
CCCGC(4/6)	Fau I
CCC/GGG	Sma I
C/CCGGG	Xma I
CCGC(-3/-1)	Aci I
CCGC/GG	Sac II
CCGCTC(-3/-3)	BsrB I
C/CGG	Hpa II
C/CGG	Msp I
/CCNGG	BssK I
/CCNGG	StyD4 I
CC/NGG	ScrF I
C/CNNGG	BsaJ I
CCNNNNN/NNGG	Bsl I
C/CRYGG	Btg I
CC/SGG	Nci I
C/CTAGG	Avr II
CCTC(7/6)	Mnl I
CCTCAGC(-5/-2)	BbvC I
CCTGCA/GG	Sbf I
CCTNAGC(-5/-2)	Bpu10 I
CC/TNAGG	Bsu36 I
CCTNN/NNNAGG	EcoN I
CC/WGG	BstN I
/CCWGG	PspG I
C/CWWGG	Sty I
(10/12)CGANNNNNNTGC(12/10)	Bcg I
CGAT/CG	Pvu I
CG/CG	BstU I
C/GGCCG	Eag I
CG/GWCCG	Rsr II
CGRY/CG	BsiE I
C/GTACG	BsiW I
CGTCTC(1/5)	BsmB I
CGWCG/	Hpy99 I
CMG/CKG	MspA1 I
CR/CCGGYG	SgrA I
C/TAG	Bfa I
(14/16)CTCAAG	BpuE I
CTCAG(9/7 and 10/8)	BspCN I
(14/16)CTCCAG	Bpm I
(8/10)CTCCTC	BseR I
C/TCGAG	PaeR7 I
C/TCGAG	Tli I
C/TCGAG	Xho I
(-1/-5)CTCGTG	BssS I
CTCTTC(1/4)	Ear I
CTGAAG(16/14)	Acu I
(7/9 and 8/10)CTGAG	BspCN I
(14/16)CTGCAC	Bsg I
CTGCA/G	Pst I
(27/25)CTGCTG	EcoP15 I
CTGGAG(16/14)	Bpm I
C/TNAG	Dde I
C/TRYAG	Sfc I
C/TTAAG	Afl II
(14/16)CTTCAG	Acu I
CTTGAG(16/14)	BpuE I
C/TYRAG	Sml I
C/YCGRG	Ava I
C/YCGRG	BsoB I
GAAGA(8/7)	Mbo II
GAAGAC(2/6)	Bbs I
(4/1)GAAGAG	Ear I
(4/1)GAAGAGC	Sap I
GAANN/NNTTC	Xmn I
GAATGC(1/-1)	Bsm I
G/AATTC	EcoR I
GACGC(5/10)	Hga I
GACGT/C	Aat II
GAC/GTC	Zra I
(-3/-3)GACGTG	BmgB I
GACN/NNGTC	PflF I
GACN/NNGTC	Tth111 I
GACNN/NNGTC	PshA I
GACNNN/NNGTC	Ahd I
GACNNNN/NNGTC	Drd I
(5/5)GACTC	Mly I
(5/4)GACTC	Ple I
(5/1)GAGAC	BsmA I
(5/1)GAGACC	Bsa I
(5/1)GAGACG	BsmB I
(-3/-3)GAGCGG	BsrB I
GAGCT/C	Sac I
(6/7)GAGG	Mnl I
GAGGAG(10/8)	BseR I
GAGTC(5/5)	Mly I
GAGTC(4/5)	Ple I
G/ANTC	Hinf I
GAT/ATC	EcoR V
GA/TC	Dpn I
/GATC	BfuC I
/GATC	Dpn II
/GATC	Mbo I
/GATC	Sau3A I
(5/4)GATCC	Alw I
(9/5)GATGC	SfaN I
(5/4)GATGG	Bcc I
GATNN/NNATC	BsaB I
G/AWTC	Tfi I
GCAATG(2/0)	BsrD I
GCAGC(8/12)	Bbv I
(8/4)GCAGGT	BfuA I
(8/4)GCAGGT	BspM I
GCAGTG(2/0)	Bts I
(10/12)GCANNNNNNTCG	Bcg I
GCANNNN/NTGC	BstAP I
GCATC(5/9)	SfaN I
GCATG/C	Sph I
(-1/1)GCATTC	Bsm I
GCC/GGC	Nae I
G/CCGGC	NgoM IV
(14/12)GCCGT	BceA I
GCCNNNN/NGGC	Bgl I
GCGAT/CGC	AsiS I
GCGATG(10/14)	BtgZ I
GCG/C	Hha I
G/CGC	HinP1 I
G/CGCGC	BssH II
(-1/-3)GCGG	Aci I
GC/GGCCGC	Not I
(6/4)GCGGG	Fau I
(10/5)GCGTC	Hga I
GC/NGC	Fnu4H I
GCN/NGC	Cac8 I
GCNNNNN/NNGC	Mwo I
GCTAG/C	Bmt I
G/CTAGC	Nhe I
GCTCTTC(1/4)	Sap I
(-2/-5)GCTGAGG	BbvC I
(12/8)GCTGC	Bbv I
(-1/-5)GCTGGG	BseY I
GC/TNAGC	Blp I
(-2/-5)GCTNAGG	Bpu10 I
G/CWGC	ApeK I
G/CWGC	Tse I
GDGCH/C	Bsp1286 I
(7/10)GGAGNNNNNGT	BsaX I
(5/6)GGATAC	BciV I
GGATC(4/5)	Alw I
G/GATCC	BamH I
GGATG(2/0)	BstF5 I
GGATG(9/13)	Fok I
GG/CC	Hae III
GG/CC	Pho I
GGCCGG/CC	Fse I
GGCCNNNN/NGGCC	Sfi I
G/GCGCC	Kas I
GG/CGCC	Nar I
GGC/GCC	Sfo I
GG/CGCGCC	Asc I
GGCGGA(11/9)	Eci I
GGGAC(10/14)	BsmF I
GGGCC/C	Apa I
G/GGCCC	PspOM I
G/GNCC	Sau96 I
GGN/NCC	Nla IV
G/GTACC	Acc65 I
GGTAC/C	Kpn I
GGTCTC(1/5)	Bsa I
GGTGA(8/7)	Hph I
G/GTNACC	BstE II
G/GWCC	Ava II
G/GYRCC	Ban I
GKGCM/C	Bme1580 I
GR/CGYC	BsaH I
GRGCY/C	Ban II
(7/12)GRTACNNNNGT	Bae I
GT/AC	Rsa I
GTA/TAC	BstZ17 I
GTATCC(6/5)	BciV I
(14/10)GTCCC	BsmF I
G/TCGAC	Sal I
GTCTC(1/5)	BsmA I
(6/2)GTCTTC	Bbs I
G/TGCAC	ApaL I
GTGCAG(16/14)	Bsg I
GT/MKAC	Acc I
/GTSAC	Tsp45 I
GTT/AAC	Hpa I
GTTT/AAAC	Pme I
(18/20)GTYGGA	Mme I
GTY/RAC	Hinc II
GWGCW/C	BsiHKA I
R/AATTY	Apo I
RCATG/Y	Nsp I
R/CCGGY	BsrF I
R/GATCY	BstY I
RGCGC/Y	Hae II
RG/GNCCY	EcoO109 I
RG/GWCCY	PpuM I
TAC/GTA	SnaB I
(7/8)TCACC	Hph I
T/CATGA	BspH I
(9/11)TCCGCC	Eci I
T/CCGGA	BspE I
TCCRAC(20/18)	Mme I
T/CGA	Taq I
TCG/CGA	Nru I
TCN/GA	Hpy188 I
TC/NNGA	Hpy188 III
T/CTAGA	Xba I
(7/8)TCTTC	Mbo II
T/GATCA	Bcl I
TG/CA	HpyCH4 V
TGC/GCA	Fsp I
TGG/CCA	Msc I
T/GTACA	BsrG I
T/TAA	Mse I
TTAAT/TAA	Pac I
TTA/TAA	Psi I
TT/CGAA	BstB I
TTT/AAA	Dra I
VC/TCGAGB	PspX I
W/CCGGW	BsaW I
YAC/GTR	BsaA I
Y/GGCCR	Eae I