U
Supplied with its own unique reaction buffer that is different from the four standard NEBuffers. Its compatibility with the four standard NEBuffers is indicated by the chart.
BSA
Supplied
with a separate vial of bovine serum albumin (10 mg/ml). To obtain
100% activity BSA should be added to the 1X reaction mix to a final
concentration of 100 µg/ml.
SAM
Supplied
with a separate vial of S-adenosyl-methionine (SAM). To obtain 100%
activity, SAM should be added to the 1X reaction mix as specified
on the product data card.
dd
When performing
a double digest with this enzyme, this NEBuffer is recommended because
it minimizes star activity.
*
Purified by scientists at SibEnzyme and
supplied with a SibEnzyme buffer (B, K, O, W or Y) which ensures 100%
activity. Its compatibility with the NEBuffer System is indicated
on the chart.
NR
This buffer
is not recommended for use with with this enzyme.
Buffer Compositions
(1X):
NEBuffer 1(yellow):
10 mM Bis
Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH
7.0 at 25°C).
NEBuffer 2 (blue):
10 mM Tris-HCl,
10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH
7.9 at 25°C).
NEBuffer 3 (red):
50 mM Tris-HCl,
10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH
7.9 at 25°C).
NEBuffer 4 (green):
20 mM Tris-acetate,
10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).
Buffers should be thawed completely and mixed thoroughly before using.