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Cross Index of Recognition Sequences

Sequences at the top of each column are written 5´ to 3´ according to convention. Open squares at the left of each row are place holders for nucleotides within a restriction endonuclease recognition sequence; arrowheads indicate the point of cleavage. Sequences of complementary strands and their cleavage sites are implied. Enzymes written in blue type recognize only one sequence while enzymes written in black type recognize multiple sequences.

Tetra- and Hexa-Nucleotide Recognition Sequences   |  Penta-Nucleotide Recognition Sequences 

Palindromic Tetra- and Hexa-Nucleotide Recognition Sequences   PDF of this table

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

Tsp509 I

Fat I

BfuC I Dpn II Mbo I Sau3A I

HpyCH4 IV

CviA II

Msp I Hpa II

Bfa I

HinP1 I

Csp6 I

Taq I

Mse I

Alu I  CviJ I

BstU I

Dpn I

Hae III Pho I  CviJ I

Rsa I

HpyCH4 V

Hha I

Tai I

Nla III

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

A T

Apo I

Hind III

Pci I  Afl III

Age I  BsrF I BsaW I

Mlu I  Afl III

Spe I

Bgl II  BstY I

Tat I

A T

Acl I

Cla I BspD I

Ase I

A T

Ssp I

Afe I

Stu I

Sca I

BfrB I

A T

AT

Nsp I

Hae II

Nsi I

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

C G

Mfe I

Nco I  Sty I Btg I

Xma I  Ava I BsoB I

Btg I

Avr II  Sty I

Eag I  Eae I

BsiW I

Sfc I

PaeR7 I Tli I Xho I  Ava I BsoB I Sml I

Sfc I

Afl II  Sml I

C G

Nde I

C G

Pml I  BsaA I

Pvu II  MspA1 I

Sma I

MspA1 I

C G

Sac II

Pvu I  BsiE I

BsiE I

C G

Pst I

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

G C

EcoR I  Apo I

NgoM IV  BsrF I

BssH II

Nhe I

BamH I  BstY I

Kas I  Ban I

PspOM I

Acc65 I  Ban I

Sal I

ApaL I

G C

BsaH I

Nar I  BsaH I

Acc I

Acc I

G C

Zra I

Ecl136 II

EcoR V

Nae I

Sfo I

BstZ17 I

Hinc II

Hpa I  Hinc II

G C

G C

Aat II

Sac I  Ban II BsiHKA I Bsp1286 I

Sph I  Nsp I

Bmt I

Bbe I  Hae II

Apa I  Ban II Bme1580 I Bsp1286 I

Kpn I

Bme1580 I Bsp1286 I BsiHKA I

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

T A

BspH I

BspE I  BsaW I

Xba I

Bcl I

Eae I

BsrG I Tat I

T A

BstB I

T A

SnaB I  BsaA I

Nru I

Fsp I

Msc I

Psi I

Dra I

T A

TA

Palindromic Penta-Nucleotide Recognition Sequences      PDF of this table      top

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

N

BssK I StyD4 I

Mae III

N

Dde I

Hinf I

Sau96 I

N

ScrF I

Fnu4H I

N

HpyCH4 II

Hpy188 I

N

Fmu I

N

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

A T

PspG I

A T

Tfi I

ApeK I Tse I

Ava II

A T

BstN I

A   T

A   T

A    T

Hpy99 I

AATT

ACGT

AGCT

ATAT

CATG

CCGG

CGCG

CTAG

GATC

GCGC

GGCC

GTAC

TATA

TCGA

TGCA

TTAA

G C

Tsp45 I

G C

G C

Nci I

G   C

G   C

AspCN I

G    C