Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalogue entry and on its data card.

Note: These diluents are not for use with Thermophilic DNA Polymerases. For diluting Thermophilic DNA Polymerases, please visit here.

Diluent Buffer A

Aat II
Acc I
Acc65 I
Aci I
Afe I
Afl II
Ahd I
Ale I
Alw I
AlwN I
Apa I
ApaL I
Apo I
Asc I
Ava I
Ava II
Bae I
BamH I
Ban I
Ban II
BbvC I
Bcc I
BceA I
Bcg I
BciV I
Bcl I
BfrB I
BfuC I
Bgl II
Blp I
Bme1580 I
Bmt I

Bpu10 I
BpuE I
BsaA I
BsaB I
BsaH I
BsaJ I
BsaW I
BseR I
BsiE I
BsiW I
Bsl I
Bsm I
BsmF I
BsoB I
Bsp1286 I
BspCN I
BspD I
BspE I
BspH I
BsrB I
BsrD I
BsrG I
BssH II
BssK I
BstAP I
BstB I
BstE II
BstF5 I
BstN I
BstU I
BstX I
BstY I

Bsu36 I
Btg I
BtgZ I
Bts I
Cla I
CspC I
Dde I
Dra I
Drd I
Eae I
Ear I
Eci I
EcoN I
Eco0109 I
EcoP15 I
EcoR V
Fat I
Fau I
Fnu4H I
Fok I
Hae II
Hae III
Hga I
Hha I
Hinf I
HinP1 I
Hpa I
Hpa II
Hpy99 I
Hpy188 I
Hpy188 III
HpyCH4 III

HpyCH4 IV
Kpn I
Mbo I
Mbo II
McrBC
Mfe I
Mlu I
Mly I
Mse I
Msl I
Msp I
Nae I
N.Alw I
Nar I
N.BbvC IA
N.BbvC IB
N.BstNB I
Nci I
Nco I
Nde I
NgoM IV
Nru I
Nsi I
Nsp I
Pac I
PaeR7 I
Pci I
PflF I
PflM I
Pho I
Ple I
Pme I

Pml I
PshA I
Psi I
PspG I
PspOM I
PspX I
Pvu II
Rsa I
Sac I
Sac II
Sal I
Sau3A I
Sau96 I
Sbf I
Sca I
ScrF I
SexA I
SgrA I
Sma I
Sml I
SnaB I
Spe I
Stu I
Sty I
Tsp45 I
Tsp509 I
Xba I
Xho I
Xma I
Xmn I
Zra I

Diluent Buffer B

Acl I
Acu I
Afl III
Alu I
ApeK I
Ase I
AsiS I
Avr II
Bbs I
Bbv I
Bfa I
BfuA I
Bgl I

Bmr I
Bpm I
Bsa I
BsaX I
BseY I
Bsg I
BsiHKA I
BsmA I
BsmB I
BspM I
Bsr I
BssS I
BstZ17 I

Cac8 I
CviA II
Dpn I
Dpn II
Dra III
Fse I
Hinc II
Hind III
Hph I
HpyCH4 V
I-Ceu I
I-Sce I

Kas I
Mme I
Mnl I
Msc I
MspA1 I
Mwo I
Nb.Bsm I
Nla III
Nla IV
PI-Psp I
PI-Sce I
PpuM I

Pvu I
SfaN I
Sfc I
Sfo I
Sph I
StyD4 I
Swa I
Taq I
Tli I
Tse I
TspR I
Tth111 I

Diluent Buffer C

Age I
BmgB I
BsrF I

EcoR I
Eag I
Fsp I

Nhe I
Not I
Pst I

Rsr II
Sap I
Sfi I

Ssp I
Tfi I
Xcm I