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Home >Technical Resource > Restriction Endonucleases > DNA Sequences and Maps

Plasmid Vectors | Viral DNA | Bacteriophage | Complete Vector Sets

The nucleotide sequence files available below are those used to the produce the plasmid vector, viral and bacteriophage maps contained in New England Biolabs catalogue as well as the tables containing the locations of sites. Configure your www browser with Adobe Acrobat as a helper application to enable it to read and print the .pdf files.

Frequencies of Restriction Sites

Plasmid Vectors	Nucleotide sequence	Restriction Maps	Location of Sites
LITMUS 28i	LITMUS 28i 2,823 bp	LITMUS 28i.pdf Map	Location of Sites
LITMUS 38i	LITMUS 38i 2,814 bp	LITMUS 38i.pdf Map	Location of Sites
LITMUS complete set
pACYC177	pACYC177 3,941 bp	pACYC177.pdf Map	Location of Sites
pACYC184	pACYC184 4,245 bp	pACYC184.pdf Map	Location of Sites
pBeloBAC11	pBeloBAC11 7,507 bp	pBeloBAC11.pdf Map	Location of Sites
pBR322	pBR322 4,361 bp	pBR322.pdf Map	Location of Sites
pET11c	pET11c 5,672 bp	pET11c.pdf Map	Promoter Diagram
pET complete set
pFOS1	pFOS1 9,738 bp	pFOS1.pdf Map	Location of Sites
pGPS1.1	pGPS1.1 4,814 bp	pGPS1.1.pdf Map
pGPS2.1	pGPS2.1 4,490 bp	pGPS2.1.pdf Map
pGPS3	pGPS3 4,293 bp	pGPS3.pdf Map	Location of Sites
pGPS4	pGPS4 3,899 bp	pGPS4.pdf Map
pGPS5	pGPS5 4,223 bp	pGPS5.pdf Map
pGPS complete set
pMAL-P2x	pMAL-P2x 6,721 bp	pMAL-P2x.pdf Map	Location of Sites
pMAL complete set
pNEB193	pNEB193 2,713 bp	pNEB193.pdf Map	Location of Sites
pNEB205A	pNEB205A 2,710 bp	pNEB205A.pdf Map
pNEB206A	pNEB206A 2,722 bp	pNEB206A.pdf Map	Location of Sites
pRS413	pRS413 4,970 bp	pRS413.pdf Map	Location of Sites
pRS4xx complete set
pTWIN1	pTWIN1 7,375 bp	pTWIN1.pdf Map	Location of Sites
pTXB1	pTXB1 6,706 bp	pTXB1.pdf Map	Location of Sites
pTYB1	pTYB1 7,477 bp	pTYB1.pdf Map	Location of Sites
pTYB11	pTYB11 7,412 bp	pTYB11.pdf Map	Location of Sites
pTYB (IMPACT) complete set
pUC19	pUC19 2,686 bp	pUC19.pdf Map	Location of Sites
YEp24	YEp24 7,769 bp	Yep24.pdf Map	Location of Sites
pNEBR-R1	pNEBR-R1 10,338 bp	pNEBR-R1.pdf Map
pNEBR-X1	pNEBR-X1 4,287 bp	pNEBR-X1.pdf Map
pNEBR-X1GLuc	pNEBR-X1GLuc 4,821 bp	pNEBR-X1GLuc.pdf Map
RheoSwitch complete set
pKLAC1 Vector	pKLAC1 9,091 bp	pKLAC1_map.pdf Map

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Viral DNA	Nucleotide Sequence	Maps	Location of Sites
Adenovirus type 2	Adenovirus-2 35,937 bp	Adenovirus-2.pdf Map	Location of Sites
SV40	SV40 5,243 bp	SV40.pdf Map	Location of Sites

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Bacteriophage	Nucleotide Sequence	Maps	Location of Sites
Lambda	Lambda 48,502 bp	lambda.pdf Map	Location of Sites
Lambda gt11 (SacI-KpnI)	lgt11 1,988 bp	lgt11.pdf Map
M13KE	M13KE 7,222 bp	M13KE.pdf Map	Location of Sites
M13KO7	M13KO7 8,669 bp
M13mp18	M13mp18 7,249 bp	M13mp18.pdf Map	Location of Sites
M13mp complete set
phiX174	phiX174 5,386 bp	phix174.pdf Map	Location of Sites
T7	T7 39,937 bp	T7.pdf Map	Location of Sites

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Vector Sets	Nucleotide Sequence
IMPACT Vector Sequence Set	IMPACT Series
LITMUS Vector Sequence Set	LITMUS Series
M13mp/pUC Polylinker Set	M13mp/pUC Series
pGPS Vector Sequence Set	pGPS Series
pGT-Nxx Vector Sequence Set	pGT-Nxx Series
pMAL Vector Sequence Set	pMAL Series
pRS3xx Vector Sequence Set	pRS3xx Series
pRS4xx Vector Sequence Set	pRS4xx Series
T7 Vector Sequence Set	pET T7 Series

Any sequences revised since the catalogue or found to be in error are noted by the addition of _rev to the sequence name. These sequences are provided as a convenience to the scientific community and New England Biolabs is not responsible for any inaccuracies present in the sequences.