Average Fragment Size Generated by Endonuclease Cleavage

Average fragment sizes were generated from long contiguous stretches of DNA sequence available from public databases. For each organism, the length of DNA sequence evaluated was: E.coli, 4.6 Mb; M.tuberculosis, 4.4 Mb; P.abyssi, 1.8 Mb; S.cerevisiae, 12.1 Mb; A.thaliana, 1.8 Mb; C.elegans, 100 Mb; D.melanogaster, 3.8 Mb; M.musculus, 2.6 Mb and H.sapiens, 22 Mb.

Enzyme	Sequence	E. coli	M. tuberculosis	P. abyssi	S. cerevisiae	A. thaliana	C. elegans	Drosophilia melanogaster	Mus musculus	Homo sapiens
Apa I	GGGCCC	68,000	3,000	5,000	15,000	42,000	38,000	13,000	5,000	5,000
Asc I	GGCGCGCC	28,000	9,000	350,000	450,000	920,000	510,000	200,000	280,000	670,000
Avr II	CCTAGG	290,000	19,000	5,000	18,000	20,000	17,000	25,000	4,000	5,000
BamH I	GGATCC	9,000	3,000	4,000	7,000	8,000	9,000	7,000	4,000	7,000
BbvC I	CCTCAGC	34,000	13,000	11,000	29,000	29,000	56,000	25,000	7,000	4,000
Bgl I	GCCNNNNNGGC	2,000	600	6,000	12,000	26,000	16,000	5,000	5,000	5,000
BssH II	GCGCGC	2,000	900	100,000	27,000	120,000	14,000	12,000	19,000	33,000
Dra I	TTTAAA	3,000	120,000	2,000	1,000	900	500	800	1,000	1,000
Eag I	CGGCCG	16,000	600	11,000	27,000	37,000	17,000	11,000	30,000	32,000
EcoR I	GAATTC	7,000	4,000	2,000	3,000	3,000	2,000	4,000	4,000	4,000
Fse I	GGCCGGCC	930,000	7,000	140,000	520,000	610,000	450,000	150,000	160,000	170,000
Hind III	AAGCTT	8,000	18,000	1,000	3,000	2,000	3,000	3,000	4,000	4,000
Nae I	GCCGGC	16,000	400	12,000	21,000	25,000	17,000	5,000	22,000	21,000
Nar I	GGCGCC	49,000	700	26000	15,000	47,000	14,000	7,000	17,000	11,000
Nhe I	GCTAGC	30,000	6,000	6,000	10,000	12,000	19,000	10,000	7,000	11,000
Not I	GCGGCCGC	200,000	4,000	180,000	290,000	610,000	260,000	83,000	120,000	130,000
Pac I	TTAATTAA	32,000	No Sites	42,000	28,000	8,000	14,000	7,000	34,000	21,000
Pme I	GTTTAAAC	53,000	2,200,000	88,000	57,000	37,000	52,000	46,000	100,000	70,000
Rsr II	CGGWCCG	12,000	3,000	140,000	82,000	77,000	62,000	28,000	120,000	260,000
Sac I	GAGCTC	31,000	4,000	2,000	8,000	5,000	5,000	6,000	3,000	5,000
Sac II	CCGCGG	7,000	700	17,000	24,000	24,000	27,000	15,000	23,000	35,000
Sal I	GTCGAC	9,000	1,000	14,000	11,000	12,000	10,000	8,000	48,000	83,000
Sap I	GCTCTTC	13,000	22,000	5,000	12,000	12,000	14,000	17,000	9,000	14,000
Sbf I	CCTGCAGG	68,000	27,000	290,000	120,000	120,000	280,000	64,000	23,000	31,000
Sfi I	GGCCN5GGCC	150,000	4,000	57,000	170,000	460,000	280,000	81,000	23,000	62,000
SgrA I	CRCCGGYG	8,000	900	55,000	46,000	34,000	59,000	27,000	98,000	170,000
Sma I	CCCGGG	11,000	2,000	12,000	42,000	40,000	31,000	15,000	7,000	7,000
Spe I	ACTAGT	59,000	45,000	8,000	5,000	5,000	8,000	10,000	9,000	8,000
Sph I	GCATGC	8,000	3,000	9,000	8,000	9,000	10,000	5,000	4,000	5,000
Srf I	GCCCGGGC	120,000	10,000	220,000	570,000	No Sites	1,100,000	170,000	120,000	120,000
Ssp I	AATATT	2,000	32,000	2,000	1,000	1,000	800	900	3,000	1,000
Swa I	ATTTAAAT	40,000	4,411,523	29,000	18,000	12,000	8,000	6,000	31,000	15,000
Xba I	TCTAGA	120,000	40,000	5,000	4,000	4,000	4,000	8,000	3,000	4,000
Xho I	CTCGAG	40,000	3,000	2,000	9,000	6,000	9,000	7,000	15,000	22,000