Isoschizomers

Restriction endonucleases that recognize the same sequence are isoschizomers. The list below contains isoschizomers for commercially available restriction endonucleases. It also specifies which isoschizomer is available from New England Biolabs. Additional prototypes that are not commercially available are also listed.

All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/".

Numbers in parentheses indicate point of cleavage for non-palindromic enzymes. For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´

Isoschizomers with alternative cleavage sites are indicated with a " ^ ".
Enzymes that are not currently commercially available are indicated with a " x ".

| A | B | C | D | E | F | G | H | I | K | L | M | N | O | P | R | S | T | U | V | X | Z |

Enzyme	Sequence	NEB Enzyme	catalogue #	Sequence	Other Isoschizomers
Aar I	CACCTGC (4/8)
Aas I	GACNNNN/NNGTC	Drd I	R0530	GACNNNN/NNGTC	Drd I, DseD I
Aat I	AGG/CCT	Stu I	R0187	AGG/CCT	Eco147 I, Pce I, SseB I, Stu I
Aat II	GACGT/C	Aat II	R0117	GACGT/C	Zra I^
		Zra I^	V0464	GAC/GTC
Acc I	GT/MKAC	Acc I	R0161	GT/MKAC	Fbl I, Xmi I
Acc II	CG/CG	BstU I	R0518	CG/CG	Bsh1236 I, BstFN I, BstU I, Mvn I
Acc III	T/CCGGA	BspE I	R0540	T/CCGGA	BseA I, Bsp13 I, BspE I, Kpn2 I, Mro I
Acc16 I	TGC/GCA	Fsp I	R0135	TGC/GCA	Avi II, Fsp I, Nsb I
Acc36 I	ACCTGC (4/8)	BfuA I	R0701	ACCTGC (4/8)	BfuA I, BspM I, Bve I
		BspM I	R0502	ACCTGC (4/8)
Acc65 I	G/GTACC	Acc65 I	R0599	G/GTACC	Asp718 I, Kpn I^
		Kpn I^	R0142	GGTAC/C
AccB1 I	G/GYRCC	Ban I	R0118	G/GYRCC	Ban I, BshN I, BspT107 I
AccB7 I	CCANNNN/NTGG	PflM I	R0509	CCANNNN/NTGG	PflB I, PflM I, Van91 I
AccBS I	CCGCTC (-3/-3)	BsrB I	R0102	CCGCTC (-3/-3)	BsrB I, Mbi I
Ace IIIx	CAGCTC (7/11)
Aci I	CCGC (-3/-1)	Aci I	R0551	CCGC (-3/-1)	Ssi I
Acl I	AA/CGTT	Acl I	R0598	AA/CGTT	Psp1406 I
AclW I	GGATC (4/5)	Alw I	R0513	GGATC (4/5)	Alw I, BspP I
Acs I	R/AATTY	Apo I	R0566	R/AATTY	Apo I, Xap I
Acu I	CTGAAG (16/14)	Acu I	R0641	CTGAAG (16/14)	Eco57 I
Acv I	CAC/GTG	Pml I	R0532	CAC/GTG	BbrP I, Eco72 I, PmaC I, Pml I, PspC I
Acy I	GR/CGYC	BsaH I	R0556	GR/CGYC	BsaH I, BstAC I, Hin1 I, Hsp92 I
Ade I	CACNNN/GTG	Dra III	R0510	CACNNN/GTG	Dra III
Afa I	GT/AC	Rsa I	R0167	GT/AC	Csp6 I^, Rsa I
Afe I	AGC/GCT	Afe I	V0213	AGC/GCT	Aor51H I, Eco47 III, Fun I
Afl II	C/TTAAG	Afl II	R0520	C/TTAAG	Bfr I, BspT I, Bst98 I, MspC I, Vha464 I
Afl III	A/CRYGT	Afl III	R0541	A/CRYGT
Age I	A/CCGGT	Age I	R0552	A/CCGGT	AsiA I, BshT I, CspA I, PinA I
Aha IIIx	TTT/AAA	Dra I	R0129	TTT/AAA	Dra I
Ahd I	GACNNN/NNGTC	Ahd I	R0584	GACNNN/NNGTC	AspE I, Dri I, Eam1105 I, EclHK I
Ahl I	A/CTAGT	Spe I	R0133	A/CTAGT	Bcu I, Spe I
Ajn I	/CCWGG	^	R0168	CC/WGG	BseB I^, BstN I^, BstO I^, Bst2U I^, EcoRI I, Mva I^, Psp6 I, PspG I
		PspG I	R0611	/CCWGG
Ale I	CACNN/NNGTG	Ale I	R0734	CACNN/NNGTG	Oli I
Alf I	(10/12)GCANNNNNNTGC (12/10)
Alo I	(7/12) GAACNNNNNNTCC (12/7)
Alu I	AG/CT	Alu I	R0137	AG/CT
Alw I	GGATC (4/5)	Alw I	R0513	GGATC (4/5)	AclW I, BspP I
Alw21 I	GWGCW/C	BsiHKA I	R0570	GWGCW/C	AspH I, Bbv12 I, BsiHKA I
Alw26 I	GTCTC (1/5)	BsmA I	R0529	GTCTC (1/5)	BsmA I, BsoMA I
Alw44 I	G/TGCAC	ApaL I	R0507	G/TGCAC	ApaL I, Vne I
AlwN I	CAGNNN/CTG	AlwN I	R0514	CAGNNN/CTG	Cai I
Ama87 I	C/YCGRG	Ava I	R0152	C/YCGRG	Ava I, BsiHKC I, BsoB I, Eco88 I, Nsp III
		BsoB I	R0586	C/YCGRG
Aor51H I	AGC/GCT	Afe I	V0213	AGC/GCT	Afe I, Eco47 III, Fun I
Apa I	GGGCC/C	Apa I	R0114	GGGCC/C	Bsp120 I^, PspOM I^
		PspOM I^	V0215	G/GGCCC
ApaB Ix	GCANNNNN/TGC	BstAP I^	V0259	GCANNNN/NTGC	BstAP I^
ApaL I	G/TGCAC	ApaL I	R0507	G/TGCAC	Alw44 I, Vne I
ApeK I	G/CWGC	ApeK I	R0643	G/CWGC	Tse I
		Tse I	R0591	G/CWGC
Apo I	R/AATTY	Apo I	R0566	R/AATTY	Acs I, Xap I
Asc I	GG/CGCGCC	Asc I	R0558	GG/CGCGCC
Ase I	AT/TAAT	Ase I	R0526	AT/TAAT	PshB I, Vsp I
AsiA I	A/CCGGT	Age I	R0552	A/CCGGT	Age I, BshT I, CspA I, PinA I
AsiS I	GCGAT/CGC	AsiS I	R0630	GCGAT/CGC	Sgf I
Asp I	GACN/NNGTC	PflF I	R0595	GACN/NNGTC	PflF I, Psy I, Tel I, Tth111 I
		Tth111 I	R0185	GACN/NNGTC
Asp700 I	GAANN/NNTTC	Xmn I	R0194	GAANN/NNTTC	MroX I, Pdm I, Xmn I
Asp718 I	G/GTACC	Acc65 I	R0599	G/GTACC	Acc65 I, Kpn I^
		Kpn I^	R0142	GGTAC/C
AspA2 I	C/CTAGG	Avr II	R0174	C/CTAGG	Avr II, Bln I, XmaJ I
AspE I	GACNNN/NNGTC	Ahd I	R0584	GACNNN/NNGTC	Ahd I, Dri I, Eam1105 I, EclHK I
AspH I	GWGCW/C	BsiHKA I	R0570	GWGCW/C	Alw21 I, Bbv12 I, BsiHKA I
AspLE I	GCG/C	Hha I	R0139	GCG/C	BstHH I, Cfo I, Hha I, Hin6 I^, HinP1 I^, HspA I^
		HinP1 I^	R0124	G/CGC
AspS9 I	G/GNCC	Sau96 I	R0165	G/GNCC	Cfr13 I, PspP I, Sau96 I
Asu Ix	G/GNCC	Sau96 I	R0165	G/GNCC	AspS9 I, Cfr13 I, PspP I, Sau96 I
Asu II	TT/CGAA	BstB I	R0519	TT/CGAA	Bpu14 I, Bsp119 I, BspT104 I, BstB I, Csp45 I, NspV,Sfu I
AsuC2 I	CC/SGG	Nci I	R0196	CC/SGG	Bcn I, Nci I
AsuHP I	GGTGA (8/7)	Hph I	R0158	GGTGA (8/7)	Hph I
AsuNH I	G/CTAGC	Bmt I^	V0458	GCTAG/C	Nhe I, Bmt I^
		Nhe I	R0131	G/CTAGC
Ava I	C/YCGRG	Ava I	R0152	C/YCGRG	Ama87 I, BsiHKC I, BsoB I, Eco88 I, Nsp III
		BsoB I	R0586	C/YCGRG
Ava II	G/GWCC	AvaI I	R0153	G/GWCC	Bme18 I, Eco47 I, Sin I, VpaK11B I
Ava IIIx	ATGCAT	BfrB I	R0625	ATG/CAT	BfrB I, EcoT22 I^, Mph1103 I^, Nsi I^, Zsp2 I^
		Nsi I^	R0127	ATGCA/T
Avi II	TGC/GCA	Fsp I	R0135	TGC/GCA	Acc16 I, Fsp I, Nsb I
Avr II	C/CTAGG	Avr II	R0174	C/CTAGG	AspA2 I, Bln I, XmaJ I
Axy I	CC/TNAGG	Bsu36 I	R0524	CC/TNAGG	Bse21 I, Bsu36 I, Eco81 I
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Bae I	(10/15) ACNNNNGTAYC (12/7)	Bae I	R0613	(10/15) ACNNNNGTAYC (12/7)
Bal I	TGG/CCA	Msc I	R0534	TGG/CCA	Mls I, MluN I, Msc I, Msp20 I
BamH I	G/GATCC	BamH I	R0136	G/GATCC
Ban I	G/GYRCC	Ban I	R0118	G/GYRCC	AccB1 I, BshN I, BspT107 I
Ban II	GRGCY/C	Ban II	R0119	GRGCY/C	Eco24 I, EcoT38 I, FriO I
Ban III	AT/CGAT	BspD I	R0557	AT/CGAT	Bsa29 I, BseC I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
Bbe I	GGCGC/C	Kas I^	R0544	G/GCGCC	Ege I^^, Ehe I^^, Kas I^, Mly113 I^^^, Nar I^^^, Sfo I^^
		Nar I^^^	R0191	GG/CGCC
		Sfo I^^	R0606	GGC/GCC
BbrP I	CAC/GTG	Pml I	R0532	CAC/GTG	Acv I, Eco72 I, PmaC I, Pml I
Bbs I	GAAGAC (2/6)	Bbs I	R0539	GAAGAC (2/6)	Bpi I, BpuA I, BstV2 I
Bbu I	GCATG/C	Sph I	R0182	GCATG/C	Pae I, SpaH I, Sph I
Bbv I	GCAGC (8/12)	Bbv I	R0173	GCAGC (8/12)	BseX I, BstV1 I
Bbv IIx	GAAGAC (2/6)	Bbs I	R0539	GAAGAC (2/6)	Bbs I, Bpi I, BpuA I, BstV2 I
Bbv12 I	GWGCW/C	BsiHKA I	R0570	GWGCW/C	Alw21 I, AspH I, BsiHKA I
BbvC I	CCTCAGC (-5/-2)	BbvC I	R0601	CCTCAGC (-5/-2)
Bcc I	CCATC(4/5)	Bcc I	R0704	CCATC(4/5)
Bce83 Ix	CTTGAG (16/14)	BpuE I	R0633	CTTGAG (16/14)	BpuE I
BceA I	ACGGC (12/14)	BceA I	R0623	ACGGC (12/14)
Bcef Ix	ACGGC (12/13)	BceA I^	R0623	ACGGC (12/14)	BceA I^
Bcg I	(10/12) CGANNNNNNTGC (12/10)	Bcg I	R0545	(10/12) CGANNNNNNTGC (12/10)
BciV I	GTATCC (6/5)	BciV I	R0596	GTATCC (6/5)	Bfu I
Bcl I	T/GATCA	Bcl I	R0160	T/GATCA	Fba I, Ksp22 I
Bcn I	CC/SGG	Nci I	R0196	CC/SGG	AsuC2 I, Nci I
Bcu I	A/CTAGT	Spe I	R0133	A/CTAGT	Ahl I, Spe I
Bet Ix	W/CCGGW	BsaW I	R0567	W/CCGGW	BsaW I
Bfa I	C/TAG	Bfa I	R0568	C/TAG	FspB I, Mae I, Xsp I
Bfi I	ACTGGG (5/4)	Bmr I	R0600	ACTGGG (5/4)	Bmr I
Bfm I	C/TRYAG	Sfc I	R0561	C/TRYAG	BstSF I, Sfc I
Bfr I	C/TTAAG	Afl II	R0520	C/TTAAG	Afl II, BspT I, Bst98 I, MspC I, Vha464 I
BfrB I	ATGCA/T	Nsi I	R0127	ATGCA/T	EcoT22 I^, Mph1103 I^, Nsi I^, Zsp2 I^
Bfu I	GTATCC (6/5)	BciV I	R0596	GTATCC (6/5)	BciV I
BfuA I	ACCTGC (4/8)	BfuA I	R0701	ACCTGC (4/8)	Acc36 I, BspM I, Bve I
		BspM I	R0502	ACCTGC (4/8)
BfuC I	/GATC	BfuC I	R0636	/GATC	Bsp143 I, BstKT I^, BstMB I, Dpn II, Kzo9 I, Mbo I, Nde II, Sau3A I
		Dpn II	R0543	/GATC
		Mbo I	R0147	/GATC
		Sau3A I	R0169	/GATC
Bgl I	GCCNNNN/NGGC	Bgl I	R0143	GCCNNNN/NGGC
Bgl II	A/GATCT	Bgl II	R0144	A/GATCT
Bin Ix	GGATC (4/5)	Alw I	R0513	GGATC (4/5)	AclW I, Alw I, BspP I
Bln I	C/CTAGG	Avr II	R0174	C/CTAGG	AspA2 I, Avr II, XmaJ I
Blp I	GC/TNAGC	Blp I	R0585	GC/TNAGC	Bpu1102 I, Bsp1720 I, Cel II
Bme18 I	G/GWCC	Ava II	R0153	G/GWCC	Ava II, Eco47 I, Sin I, VpaK11B I
Bme1390 I	CC/NGG	BssK I^	R0592	/CCNGG	BssK I^, BstSC I^, MspR9 I, ScrF I, StyD4 I^
		ScrF I	R0110	CC/NGG
		StyD4 I^	R0638	/CCNGG
Bme1580 I	GKGCM/C	Bme1580 I	R0702	GKGCM/C	BseS I
Bmg Ix	GKGCCC
BmgB I	CACGTC (-3/-3)	BmgB I	R0628	CACGTC (-3/-3)	Btr I
Bmr I	ACTGGG (5/4)	Bmr I	R0600	ACTGGG (5/4)	Bfi I
Bmt I	GCTAG/C	Bmt I	V0458	GCTAG/C	AsuNH I^, Nhe I^
		Nhe I^	R0131	G/CTAGC
Bmy I	GDGCH/C	Bsp1286 I	R0120	GDGCH/C	Bsp1286 I, Mhl I, Sdu I
Box I	GACNN/NNGTC	PshA I	R0593	GACNN/NNGTC	BstPA I, PshA I
Bpi I	GAAGAC (2/6)	Bbs I	R0539	GAAGAC (2/6)	Bbs I, BpuA I, BstV2 I
Bpl I	(8/13) GAGNNNNNCTC (13/8)
Bpm I	CTGGAG (16/14)	Bpm I	R0565	CTGGAG (16/14)	Gsu I
Bpu10 I	CCTNAGC (-5/-2)	Bpu10 I	V0149	CCTNAGC (-5/-2)
Bpu14 I	TT/CGAA	BstB I	R0519	TT/CGAA	Asu II, Bsp119 I, BspT104 I, BstB I, Csp45 I, Nsp V,Sfu I
Bpu1102 I	GC/TNAGC	Blp I	R0585	GC/TNAGC	Blp I, Bsp1720 I, Cel II
BpuA I	GAAGAC (2/6)	Bbs I	R0539	GAAGAC (2/6)	Bbs I, Bpi I, BstV2 I
BpuE I	CTTGAG (16/14)	BpuE I	R0633	CTTGAG (16/14)
Bsa I	GGTCTC (1/5)	Bsa I	R0535	GGTCTC (1/5)	Bso31 I, BspTN I, Eco31 I
Bsa29 I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, BseC I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
BsaA I	YAC/GTR	BsaA I	R0531	YAC/GTR	BstBA I
BsaB I	GATNN/NNATC	BsaB I	R0537	GATNN/NNATC	Bse8 I, BseJ I, Mam I
BsaH I	GR/CGYC	BsaH I	R0556	GR/CGYC	Acy I, BstAC I, Hin1 I, Hsp92 I
BsaJ I	C/CNNGG	BsaJ I	R0536	C/CNNGG	BseD I, BssEC I
BsaM I	GAATGC (1/-1)	Bsm I	R0134	GAATGC (1/-1)	Bsm I, Mva1269 I, Pct I
BsaW I	W/CCGGW	BsaW I	R0567	W/CCGGW
BsaX I	(9/12) ACNNNNNCTCC (10/7)	BsaX I	R0609	(9/12) ACNNNNNCTCC (10/7)
Bsb Ix	CAACAC
Bsc4 I	CCNNNNN/NNGG	Bsl I	R0555	CCNNNNN/NNGG	BseL I, BsiY I, Bsl I
BscG Ix	CCCGT
Bse1 I	ACTGG (1/-1)	Bsr I	R0527	ACTGG (1/-1)	BseN I, Bsr I, BsrS I
Bse8 I	GATNN/NNATC	BsaB I	R0537	GATNN/NNATC	BsaB I, BseJ I, Mam I
Bse21 I	CC/TNAGG	Bsu36 I	R0524	CC/TNAGG	Axy I, Bsu36 I, Eco81 I
Bse118 I	R/CCGGY	BsrF I	R0562	R/CCGGY	BsrF I, BssA I, Cfr10 I
BseA I	T/CCGGA	BspE I	R0540	T/CCGGA	Acc III, Bsp13 I, BspE I, Kpn2 I, Mro I
BseB I	CC/WGG	BstN I	R0168	CC/WGG	Ajn I^, BstN I, BstO I, Bst2U I, EcoR II^, Mva I, Psp6 I^, PspG I^
		PspG I^	R0611	/CCWGG
BseC I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
BseD I	C/CNNGG	BsaJ I	R0536	C/CNNGG	BsaJ I, BssEC I
Bse3D I	GCAATG (2/0)	BsrD I	R0574	GCAATG (2/0)	BseM I, BsrD I
BseG I	GGATG (2/0)	BstF5 I	V0031	GGATG (2/0)	BstF5 I, Fok I^
		Fok I^	R0109	GGATG (9/13)
BseJ I	GATNN/NNATC	BsaB I	R0537	GATNN/NNATC	BsaB I, Bse8 I, Mam I
BseL I	CCNNNNN/NNGG	Bsl I	R0555	CCNNNNN/NNGG	Bsc4 I, BsiY I, Bsl I
BseM I	GCAATG (2/0)	BsrD I	R0574	GCAATG (2/0)	Bse3D I, BsrD I
BseM II	CTCAG (10/8)	BspCN I^	R0624	CTCAG (9/7)	BspCN I^
BseN I	ACTGG (1/-1)	Bsr I	R0527	ACTGG (1/-1)	Bse1 I, Bsr I, BsrS I
BseP I	G/CGCGC	BssH II	R0199	G/CGCGC	BssH II, Pau I
BseR I	GAGGAG (10/8)	BseR I	R0581	GAGGAG (10/8)
BseS I	GKGCM/C	Bme1580 I	R0702	GKGCM/C	Bme1580 I
BseX I	GCAGC (8/12)	Bbv I	R0173	GCAGC (8/12)	Bbv I, BstV1 I
BseX3 I	C/GGCCG	Eag I	R0505	C/GGCCG	BstZ I, Eag I, EclX I, Eco52 I
BseY I	CCCAGC (-5/-1)	BseY I	R0635	CCCAGC (-5/-1)
Bsg I	GTGCAG (16/14)	Bsg I	R0559	GTGCAG (16/14)
Bsh1236 I	CG/CG	BstU I	R0518	CG/CG	Acc II, BstFN I, BstU I, Mvn I
Bsh1285 I	CGRY/CG	BsiE I	R0554	CGRY/CG	BsiE I, BstMC I
BshF I	GG/CC	Hae III	R0108	GG/CC	BspAN I, BsuR I, Hae III, Pal I, Pho I
		Pho I	R0705	GG/CC
BshN I	G/GYRCC	Ban I	R0118	G/GYRCC	AccB1 I, Ban I, BspT107 I
BshT I	A/CCGGT	Age I	R0552	A/CCGGT	Age I, AsiA I, CspA I, PinA I
Bsi Ix	CACGAG (-5/-1)	BssS I	R0587	CACGAG (-5/-1)	BssS I, Bst2B I
BsiE I	CGRY/CG	BsiE I	R0554	CGRY/CG	Bsh1285 I, BstMC I
BsiHKA I	GWGCW/C	BsiHKA I	R0570	GWGCW/C	Alw21 I, AspH I, Bbv12 I
BsiHKC I	C/YCGRG	Ava I	R0152	C/YCGRG	Ama87 I, Ava I, BsoB I, Eco88 I, Nsp III
		BsoB I	R0586	C/YCGRG
BsiS I	C/CGG	Hpa II	R0171	C/CGG	Hap II, Hpa II, Msp I
		Msp I	R0106	C/CGG
BsiW I	C/GTACG	BsiW I	R0553	C/GTACG	Pfl23 II, PspL I, Sun I
		Cla I	R0197	AT/CGAT
BsiY I	CCNNNNN/NNGG	Bsl I	R0555	CCNNNNN/NNGG	Bsc4 I, BseL I, Bsl I
Bsl I	CCNNNNN/NNGG	Bsl I	R0555	CCNNNNN/NNGG	Bsc4 I, BseL I, BsiY I
BslF I	(11/15)GGGAC(10/14)	BsmF I	R0572	GGGAC(10/14)	BsmF I
Bsm I	GAATGC (1/-1)	Bsm I	R0134	GAATGC (1/-1)	BsaM I, Mva1269 I, Pct I
BsmA I	GTCTC (1/5)	BsmA I	R0529	GTCTC (1/5)	Alw26 I, BsoMA I
BsmB I	CGTCTC (1/5)	BsmB I	R0580	CGTCTC (1/5)	Esp3 I
BsmF I	GGGAC (10/14)	BsmF I	R0572	GGGAC (10/14)	BslF I
Bso31 I	GGTCTC (1/5)	Bsa I	R0535	GGTCTC (1/5)	Bsa I, BspTN I, Eco31 I
BsoB I	C/YCGRG	Ava I	R0152	C/YCGRG	Ama87 I, Ava I, BsiHKC I, Eco88 I, Nsp III
		BsoB I	R0586	C/YCGRG
BsoMA I	GTCTC (1/5)	BsmA I	R0529	GTCTC (1/5)	Alw26 I, BsmA I
Bsp13 I	T/CCGGA	BspE I	R0540	T/CCGGA	Acc III, BseA I, BspE I, Kpn2 I, Mro I
Bsp19 I	C/CATGG	Nco I	R0193	C/CATGG	Nco I
Bsp24 Ix	(8/13) GACNNNNNNTGG (12/7)
Bsp68 I	TCG/CGA	Nru I	R0192	TCG/CGA	Nru I
Bsp106 I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, Bsa29 I, BseC I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
Bsp119 I	TT/CGAA	BstB I	R0519	TT/CGAA	Asu II, Bpu14 I, BspT104 I, BstB I, Csp45 I, NspV,Sfu I
Bsp120 I	G/GGCCC	Apa I^	R0114	GGGCC/C	Apa I^, PspOM I
		PspOM I	V0215	G/GGCCC
Bsp143 I	/GATC	BfuC I	R0636	/GATC	BfuC I, BstKT I^, BstMB I, Dpn II, Kzo9 I, Mbo I, Nde II, Sau3A I
		Dpn II	R0543	/GATC
		Mbo I	R0147	/GATC
		Sau3A I	R0169	/GATC
Bsp143 II	RGCGC/Y	Hae II	R0107	RGCGC/Y	BstH2 I, Hae II
Bsp1286 I	GDGCH/C	Bsp1286 I	R0120	GDGCH/C	Bmy I, Mhl I, Sdu I
Bsp1407 I	T/GTACA	BsrG I	R0575	T/GTACA	BsrG I, BstAU I, SspB I
Bsp1720 I	GC/TNAGC	Blp I	R0585	GC/TNAGC	Blp I, Bpu1102 I, Cel II
BspAN I	GG/CC	Hae III	R0108	GG/CC	BshF I, BsuR I, Hae III, Pal I, Pho I
		Pho I	R0705	GG/CC
BspC I	CGAT/CG	Pvu I	R0150	CGAT/CG	Ple19 I, Pvu I
BspCN I	CTCAG (9/7)	BspCN I	R0624	CTCAG (9/7)	BseM II^
BspD I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, Bsa29 I, BseC I, Bsp106 I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
BspE I	T/CCGGA	BspE I	R0540	T/CCGGA	Acc III, BseA I, Bsp13 I, Kpn2 I, Mro I
BspG Ix	CTGGAC
BspH I	T/CATGA	BspH I	R0517	T/CATGA	Pag I, Rca I
BspL I	GGN/NCC	Nla IV	R0126	GGN/NCC	Nla IV, PspN4 I
BspLU11 I	A/CATGT	Pci I	V0275	A/CATGT	Pci I
BspM I	ACCTGC (4/8)	BfuA I	R0701	ACCTGC (4/8)	Acc36 I, BfuA I, Bve I
		BspM I	R0502	ACCTGC (4/8)
BspM IIx	T/CCGGA	BspE I	R0540	T/CCGGA	Acc III, BseA I, Bsp13 I, BspE I, Kpn2 I, Mro I
BspMA I	CTGCA/G	Pst I	R0140	CTGCA/G	Pst I
BspNC Ix	CCAGA
BspP I	GGATC (4/5)	Alw I	R0513	GGATC (4/5)	AclW I, Alw I
BspT I	C/TTAAG	Afl II	R0520	C/TTAAG	Afl II, Bfr I, Bst98 I, MspC I, Vha464 I
BspT104 I	TT/CGAA	BstB I	R0519	TT/CGAA	Asu II, Bpu14 I, Bsp119 I, BstB I, Csp45 I, NspV,Sfu I
BspT107 I	G/GYRCC	Ban I	R0118	G/GYRCC	AccB1 I, Ban I, BshN I
BspTN I	GGTCTC (1/5)	Bsa I	R0535	GGTCTC (1/5)	Bsa I, Bso31 I, Eco31 I
BspX I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, Bsa29 I, BseC I, Bsp106 I, BspD I, Bsu15 I, BsuTU I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
Bsr I	ACTGG (1/-1)	Bsr I	R0527	ACTGG (1/-1)	Bse1 I, BseN I, BsrS I
BsrB I	CCGCTC (-3/-3)	BsrB I	R0102	CCGCTC (-3/-3)	AccBS I, Mbi I
BsrD I	GCAATG (2/0)	BsrD I	R0574	GCAATG (2/0)	Bse3D I, BseM I
BsrF I	R/CCGGY	BsrF I	R0562	R/CCGGY	Bse118 I, BssA I, Cfr10 I
BsrG I	T/GTACA	BsrG I	R0575	T/GTACA	Bsp1407 I, BstAU I, SspB I
BsrS I	ACTGG (1/-1)	Bsr I	R0527	ACTGG (1/-1)	Bse1 I, BseN I, Bsr I
BssA I	R/CCGGY	BsrF I	R0562	R/CCGGY	Bse118 I, BsrF I, Cfr10 I
BssEC I	C/CNNGG	BsaJ I	R0536	C/CNNGG	BsaJ I, BseD I
BssH I	C/TCGAG	PaeR7 I	R0177	C/TCGAG	PaeR7 I, Sfr274 I, Sla I, Tli I, Xho I
		Tli I	R0612	C/TCGAG
		Xho I	R0146	C/TCGAG
BssH II	G/CGCGC	BssH II	R0199	G/CGCGC	BseP I, Pau I
BssK I	/CCNGG	BssK I	R0592	/CCNGG	Bme1390 I^, BstSC I, MspR9 I^, ScrF I^, StyD4 I
		ScrF I^	R0110	CC/NGG
		StyD4 I	R0638	/CCNGG
BssNA I	GTA/TAC	BstZ17 I	R0594	GTA/TAC	Bst1107 I, BstZ17 I
BssS I	CACGAG (-5/-1)	BssS I	R0587	CACGAG (-5/-1)	Bst2B I
BssT1 I	C/CWWGG	Sty I	R0500	C/CWWGG	Eco130 I, EcoT14 I, Erh I, Sty I
Bst6 I	CTCTTC (1/4)	Ear I	R0528	CTCTTC (1/4)	Eam1104 I, Ear I, Ksp632 I
Bst98 I	C/TTAAG	Afl II	R0520	C/TTAAG	Afl II, Bfr I, BspT I, MspC I, Vha464 I
Bst1107 I	GTA/TAC	BstZ17 I	R0594	GTA/TAC	BssNA I, BstZ17 I
BstAC I	GR/CGYC	BsaH I	R0556	GR/CGYC	Acy I, BsaH I, Hin1 I, Hsp92 I
BstAP I	GCANNNN/NTGC	BstAP I	V0259	GCANNNN/NTGC
BstB I	TT/CGAA	BstB I	R0519	TT/CGAA	Asu II, Bpu14 I, Bsp119 I, BspT104 I, Csp45 I, NspV,Sfu I
Bst2B I	CACGAG (-5/-1)	BssS I	R0587	CACGAG (-5/-1)	BssS I
BstBA I	YAC/GTR	BsaA I	R0531	YAC/GTR	BsaA I
Bst4C I	ACN/GT	HpyCH4 III	R0618	ACN/GT	HpyCH4 III, Taa I
BstC8 I	GCN/NGC	Cac8 I	R0579	GCN/NGC	Cac8 I
BstDE I	C/TNAG	Dde I	R0175	C/TNAG	Dde I
BstDS I	C/CRYGG	Btg I	R0608	C/CRYGG	Btg I
BstE II	G/GTNACC	BstE II	R0162	G/GTNACC	BstP I, Eco91 I, EcoO65 I, PspE I
BstEN I	CCTNN/NNNAGG	EcoN I	R0521	CCTNN/NNNAGG	EcoN I, Xag I
BstF5 I	GGATG (2/0)	BstF5 I	V0031	GGATG (2/0)	BseG I, Fok I^
		Fok I^	R0109	GGATG (9/13)
BstFN I	CG/CG	BstU I	R0518	CG/CG	AccI I, Bsh1236 I, BstU I, Mvn I
BstH2 I	RGCGC/Y	Hae II	R0107	RGCGC/Y	Bsp143 II, Hae II
BstHH I	GCG/C	Hha I	R0139	GCG/C	AspLE I, Cfo I, Hha I, Hin6 I^, HinP1 I^, HspA I^
		HinP1 I^	R0124	G/CGC
BstKT I	GAT/C	BfuC I^	R0636	/GATC	BfuC I^, Bsp143 I^, BstMB I^, Dpn II^, Kzo9 I^, Mbo I^, NdeI I^, Sau3A I^
		Dpn II^	R0543	/GATC
		Mbo I^	R0147	/GATC
		Sau3A I^	R0169	/GATC
BstMB I	/GATC	BfuC I	R0636	/GATC	BfuC I, Bsp143 I, BstKT I^, Dpn II, Kzo9 I, Mbo I, Nde II, Sau3A I
		Dpn II	R0543	/GATC
		Mbo I	R0147	/GATC
		Sau3A I	R0169	/GATC
BstMC I	CGRY/CG	BsiE I	R0554	CGRY/CG	Bsh1285 I, BsiE I
BstMW I	GCNNNNN/NNGC	Mwo I	R0573	GCNNNNN/NNGC	HpyF10V I, Mwo I
BstN I	CC/WGG	BstN I	R0168	CC/WGG	Ajn I^, BseB I, BstO I, Bst2U I, EcoR II^, Mva I, Psp6 I^, PspG I^
		PspG I^	R0611	/CCWGG
BstNS I	RCATG/Y	Nsp I	R0602	RCATG/Y	Nsp I, Xce I
BstO I	CC/WGG	BstN I	R0168	CC/WGG	Ajn I^, BseB I, BstN I, Bst2U I, EcoR II^, Mva I, PspG I^
		PspG I^	R0611	/CCWGG
BstP I	G/GTNACC	BstE II	R0162	G/GTNACC	BstE II, Eco91 I, EcoO65 I, PspE I
BstPA I	GACNN/NNGTC	PshA I	R0593	GACNN/NNGTC	Box I, PshA I
BstSC I	/CCNGG	BssK I	R0592	/CCNGG	Bme1390 I^, BssK I, MspR9 I^, ScrF I^, StyD4 I
		ScrF I^	R0110	CC/NGG
		StyD4 I	R0638	/CCNGG
BstSF I	C/TRYAG	Sfc I	R0561	C/TRYAG	Bfm I, Sfc I
BstSN I	TAC/GTA	SnaB I	R0130	TAC/GTA	Eco105 I, SnaB I
BstU I	CG/CG	BstU I	R0518	CG/CG	Acc II, Bsh1236 I, BstFN I, Mvn I
Bst2U I	CC/WGG	BstN I	R0168	CC/WGG	Ajn I^, BseB I, BstN I, BstO I, EcoRI I^, Mva I, Psp6 I^, PspG I^
		PspG I^	R0611	/CCWGG
BstV1 I	GCAGC (8/12)	Bbv I	R0173	GCAGC (8/12)	Bbv I, BseX I
BstV2 I	GAAGAC (2/6)	Bbs I	R0539	GAAGAC (2/6)	Bbs I, Bpi I, BpuA I
BstX I	CCANNNNN/NTGG	BstX I	R0113	CCANNNNN/NTGG
BstX2 I	R/GATCY	BstY I	R0523	R/GATCY	BstY I, Mfl I, Psu I, Xho II
BstY I	R/GATCY	BstY I	R0523	R/GATCY	BstX2 I, Mfl I, Psu I, Xho II
BstZ I	C/GGCCG	Eag I	R0505	C/GGCCG	BseX3 I, Eag I, EclX I, Eco52 I
BstZ17 I	GTA/TAC	BstZ17 I	R0594	GTA/TAC	BssNA I, Bst1107 I
Bsu15 I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, Bsa29 I, BseC I, Bsp106 I, BspD I, BspX I, BsuTU I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
Bsu36 I	CC/TNAGG	Bsu36 I	R0524	CC/TNAGG	Axy I, Bse21 I, Eco81 I
BsuR I	GG/CC	Hae III	R0108	GG/CC	BshF I, BspAN I, Hae III, Pal I, Pho I
		Pho I	R0705	GG/CC
BsuTU I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, Bsa29 I, BseC I, Bsp106 I, BspD I, BspX I, Bsu15 I, Cla I, Zho I
		Cla I	R0197	AT/CGAT
Btg I	C/CRYGG	Btg I	R0608	C/CRYGG	BstDS I
BtgZ I	GCGATG(10/14)	BtgZ I	R0703	GCGATG(10/14)
Btr I	CACGTC (-3/-3)	BmgB I	R0628	CACGTC (-3/-3)	BmgB I
Bts I	GCAGTG (2/0)	Bts I	R0614	GCAGTG (2/0)
Bve I	ACCTGC(4/8)	BfuA I	R0701	ACCTGC(4/8)	Acc36 I, BfuA I, BspM I
		BspM I	R0502	ACCTGC(4/8)
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Cac8 I	GCN/NGC	Cac8 I	R0579	GCN/NGC	BstC8 I
Cai I	CAGNNN/CTG	AlwN I	R0514	CAGNNN/CTG	AlwN I
Cau IIx	CC/SGG	Nci I	R0196	CC/SGG	AsuC2 I, Bcn I, Nci I
CciN I	GC/GGCCGC	Not I	R0189	GC/GGCCGC	Not I
Cdi Ix	CATCG(-1/-1)
Cel II	GC/TNAGC	Blp I	R0585	GC/TNAGC	Blp I, Bpu1102 I, Bsp1720 I
Cfo I	GCG/C	Hha I	R0139	GCG/C	AspLE I, BstHH I, Hha I, Hin6 I^, HinP1 I^, HspA I^
		HinP1 I^	R0124	G/CGC
Cfr I	Y/GGCCR	Eae I	R0508	Y/GGCCR	Eae I
Cfr9 I	C/CCGGG	Sma I^	R0141	CCC/GGG	PspA I, Sma I^, Xma I, XmaC I
		Xma I	R0180	C/CCGGG
Cfr10 I	R/CCGGY	BsrF I	R0562	R/CCGGY	Bse118 I, BsrF I, BssA I
Cfr13 I	G/GNCC	Sau96 I	R0165	G/GNCC	AspS9 I, PspP I, Sau96 I
Cfr42 I	CCGC/GG	Sac II	R0157	CCGC/GG	Ksp I, Sac II, Sfr303 I, SgrB I
Cje Ix	(8/14) CCANNNNNNGT (15/9)
CjeP Ix	(7/13) CCANNNNNNNTC (14/8)
Cla I	AT/CGAT	BspD I	R0557	AT/CGAT	Ban III, Bsa29 I, BseC I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Zho I
		Cla I	R0197	AT/CGAT
Cpo I	CG/GWCCG	Rsr II	R0501	CG/GWCCG	Csp I, Rsr II, Rsr2 I
Csp I	CG/GWCCG	Rsr II	R0501	CG/GWCCG	Cpo I, Rsr II, Rsr2 I
Csp6 I	G/TAC	Rsa I^	R0167	GT/AC	Afa I^, Rsa I^
Csp45 I	TT/CGAA	BstB I	R0519	TT/CGAA	Asu II, Bpu14 I, Bsp119 I, BspT104 I, BstB I, NspV,Sfu I
CspA I	A/CCGGT	Age I	R0552	A/CCGGT	Age I, AsiA I, BshT I, PinA I
CspC I	(11/13)CAANNNNNGTGG(12/10)	CspC I	R0645	(11/13)CAANNNNNGTGG(12/10)
CstM Ix	AAGGAG(20/18)
CviA II	C/ATG	CviA II	R0640	C/ATG	Fat I^, Hsp92 II^^, Nla III^^
		^	V0156	/CATG
		^	R0125	CATG/
CviJ I	RG/CY				CviT I
CviR I	TG/CA	HpyCH4 V	R0620	TG/CA	HpyCH4 V
CviT I	RG/CY				CviJ I
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Dde I	C/TNAG	Dde I	R0175	C/TNAG	BstDE I
Dpn I	GA/TC	Dpn I	R0176	GA/TC
Dpn II	/GATC	BfuC I	R0636	/GATC	BfuC I, Bsp143 I, BstKT I^, BstMB I, Kzo9 I, Mbo I, Nde II, Sau3A I
		Dpn II	R0543	/GATC
		Mbo I	R0147	/GATC
		Sau3A I	R0169	/GATC
Dra I	TTT/AAA	Dra I	R0129	TTT/AAA
Dra II	RG/GNCCY	EcoO109 I	R0503	RG/GNCCY	EcoO109 I
Dra III	CACNNN/GTG	Dra III	R0510	CACNNN/GTG	Ade I
Drd I	GACNNNN/NNGTC	Drd I	R0530	GACNNNN/NNGTC	Aas I, DseD I
Drd IIx	GAACCA
Dri I	GACNNN/NNGTC	Ahd I	R0584