| Enzymes | Recognition Sequence | |
| D | Acc I | GT/MKAC |
| D | Afl III | A/CRYGT |
| D | ApeK I | G/CWGC |
| D | Apo I | R/AATTY |
| D | Ava I | C/YCGRG |
| D | Ava II | G/GWCC |
| D | Bae I | (10/15) ACNNNNGTAYC (12/7) |
| D | Ban I | G/GYRCC |
| D | Ban II | GRGCY/C |
| D | Bme 1580 I | GKGCM/C |
| X | Bmg I | GKGCCC |
| D | BsaA I | YAC/GTR |
| D | BsaH I | GR/CGYC |
| D | BsaW I | W/CCGGW |
| D | BsiE I | CGRY/CG |
| D | BsiHKA I | GWGCW/C |
| D | BsoB I | C/YCGRG |
| D | Bsp1286 I | GDGCH/C |
| D | BsrF I | R/CCGGY |
| D | BstN I | CC/WGG |
| D | BstY I | R/GATCY |
| D | Btg I | C/CRYGG |
| CviJ I | RG/CY | |
| D | Eae I | Y/GGCCR |
| X | EcoH I | /CCSGG |
| Eco57M I | CTGRAG (16/14) | |
| D | EcoO109 I | RG/GNCCY |
| FspA I | RTGC/GCAY | |
| X | Gdi II | CGGCCR (-5/-1) |
| X | Hae I | WGG/CCW |
| D | Hae II | RGCGC/Y |
| X | Hae IV | (7/13) GAYNNNNNRTC (14/9) |
| Hin4 I | (8/13) GAYNNNNNVTC (13/8) | |
| D | Hinc II | GTY/RAC |
| D | Hpy99 I | CGWCG/ |
| X | Lpn I | RGC/GCY |
| D | Mme I | TCCRAC (20/18) |
| D | Msl I | CAYNN/NNRTG |
| D | MspA1 I | CMG/CKG |
| D | Nci I | CC/SGG |
| X | Nli3877 I | CYCGR/G |
| D | Nsp I | RCATG/Y |
| X | Pas I | CC/CWGGG |
| D | PpuM I | RG/GWCCY |
| X | Psp03 I | GGWC/C |
| D | PspG I | /CCWGG |
| D | PspX I | VC/TCGAGB |
| X | Pss I | RGGNC/CY |
| D | Rsr II | CG/GWCCG |
| SanD I | GG/GWCCC | |
| D | SexA I | A/CCWGGT |
| D | Sfc I | C/TRYAG |
| D | SgrA I | CR/CCGGYG |
| D | Sml I | C/TYRAG |
| X | Sse8647 I | AG/GWCCT |
| D | Sty I | C/CWWGG |
| Tat I | W/GTACW | |
| Tau I | GCSG/C | |
| D | Tfi I | G/AWTC |
| D | Tse I | G/CWGC |
| D | Tsp45 I | /GTSAC |
| D | TspR I | CASTGNN/ |
| X | Tth111 II | CAARCA (11/9) |
| X | UbaF1 I | TARCCA |
| X | UbaF2 I | GAAAYNNNNNRTG |
| X | VpaK11A I | /GGWCC |
Example:
Acc I recognizes each of the following sequences:
GTAGAC
GTATAC
GTCGAC
GTCTAC
Note:
Enzymes marked with an ( D ) are available
from NEB.
Enzymes marked with an ( X ) are not currently commercially available