Home Technical Resource > Restriction Endonucleases >
Nonpalindromic Sequences
| Enzymes | Recognition Sequence | |
| Aar I | CACCTGC(4/8) | |
| X | Ace III | CAGCTC(7/11) |
| D | Aci I | CCGC(-3/-1) |
| D | Acu I | CTGAAG(16/14) |
| Alo I | (7/12)GAACNNNNNNTCC(12/7) | |
| D | Alw I | GGATC(4/5) |
| AspCN I | GCCGC | |
| D | Bae I | (10/15)ACNNNNGTAYC(12/7) |
| X | Bbr7 I | GAAGAC(7/11) |
| D | Bbs I | GAAGAC(2/6) |
| D | Bbv I | GCAGC(8/12) |
| D | BbvC I | CCTCAGC(-5/-2) |
| D | Bcc I | CCATC |
| D | BceA I | ACGGC(12/14) |
| X | Bcef I | ACGGC(12/13) |
| D | Bcg | (10/12)CGANNNNNNTGC(12/10) |
| D | BciV I | GTATCC(6/5) |
| D | BfuA I | ACCTGC(4/8) |
| X | Bmg I | GKGCCC |
| D | BmgB I | CACGTC(-3/-3) |
| D | Bmr I | ACTGGG(5/4) |
| D | Bpm I | CTGGAG(16/14) |
| D | Bpu10 I | CCTNAGC(-5/-2) |
| D | BpuE I | CTTGAG(16/14) |
| D | Bsa I | GGTCTC(1/5) |
| D | BsaX I | (9/12)ACNNNNNCTCC(10/7) |
| X | Bsb I | CAACAC |
| X | BscA I | GCATC(4/6) |
| X | BscG I | CCCGT |
| BseM II | CTCAG(10/8) | |
| D | BseR I | GAGGAG(10/8) |
| D | BseY I | C/CCAGC |
| D | Bsg I | GTGCAG(16/14) |
| BslF I | (11/15)GGGAC(10/14) | |
| D | Bsm I | GAATGC(1/-1) |
| D | BsmA I | GTCTC(1/5) |
| D | BsmB I | CGTCTC(1/5) |
| D | BsmF I | GGGAC(10/14) |
| X | Bsp24 I | (8/13)GACNNNNNNTGG(12/7) |
| D | BcpCN I | CTCAG(10/8 and 9/7) |
| X | BspG I | CTGGAC |
| D | BspM I | ACCTGC(4/8) |
| X | BspNC I | CCAGA |
| D | Bsr I | ACTGG(1/-1) |
| D | BsrB I | CCGCTC(-3/-3) |
| D | BsrD I | GCAATG(2/0) |
| D | BssS I | CACGAG(-5/-1) |
| D | BstF5 I | GGATG(2/0) |
| D | BtgZ I | GCGATG(10/14) |
| D | Bts I | GCAGTG(2/0) |
| X | Cdi I | CATCG(-1/-1) |
| X | Cje I | (8/14)CCANNNNNNGT(15/9) |
| X | CjeP I | (7/13)CCANNNNNNNTC(14/8) |
| D | CspC I | (11/13CAANNNNNGTGG(12/10) |
| X | CstM I | AAGGAG(20/18) |
| X | Drd II | GAACCA |
| D | Ear I | CTCTTC(1/4) |
| D | Eci I | GGCGGA(11/9) |
| Eco57M I | CTGRAG(16/14) | |
| D | EcoP15 I | CAGCAG(25/27) |
| D | Fau I | CCCGC(4/6) |
| X | Fin I | GGGAC |
| D | Fok I | GGATG(9/13) |
| X | Gdi II | CGGCCR(-5/-1) |
| D | Hga I | GACGC(5/10) |
| Hin4 I | (8/13)GAYNNNNNVTC(13/8) | |
| X | Hin4 II | CCTTC |
| D | Hph I | GGTGA(8/7) |
| X | HpyAV | CCTTC(6/5) |
| D | Mbo II | GAAGA(8/7) |
| D | Mly I | GAGTC(5/5) |
| D | Mme I | TCCRAC(20/18) |
| D | Mnl I | CCTC(7/6) |
| X | Pfl1108 I | TCGTAG |
| D | Ple I | GAGTC(4/5) |
| Ppi I | (7/12)GAACNNNNNCTC(13/8) | |
| Psr I | (7/12)GAACNNNNNNTAC(12/7) | |
| X | RleA I | CCCACA(12/9) |
| D | Sap I | GCTCTTC(1/4) |
| D | SfaN I | GCATC(5/9) |
| X | Sim I | GGGTC(-3/0) |
| X | SspD5 I | GGTGA(8/8) |
| X | Sth132 I | CCCG(4/8) |
| X | Sts I | GGATG(10/14) |
| Taq II | GACCGA(11/9), CACCCA(11/9) | |
| TspDT I | ATGAA(11/9) | |
| TspGW I | ACGGA(11/9) | |
| X | Tth111 II | CAARCA(11/9) |
| X | UbaF1 I | TARCCA |
| X | UbaF2 I | GAAAYNNNNNRTG |
| X | UbaP I | CGAACG |
Note:
Enzymes marked with an ( D ) are available
from NEB.
Enzymes marked with an ( X ) are not currently commercially available