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Interrupted Palindromes
| Enzymes | Recognition Sequence | |
| D | Ahd I | GACNNN/NNGTC |
| D | Ale I | CACNN/NNGTG |
| Alf I | (10/12)GCANNNNNNTGC(12/10) | |
| D | AlwN I | CAGNNN/CTG |
| X | ApaB I | GCANNNNN/TGC |
| D | ApeK I | G/GWCC |
| D | Ava II | G/GWCC |
| D | Bgl I | GCCNNNN/NGGC |
| D | Blp I | GC/TNAGC |
| Bpl I | (8/13)GAGNNNNNCTC(13/8) | |
| D | BsaB I | GATNN/NNATC |
| D | BsaJ I | C/CNNGG |
| D | BsaW I | W/CCGGW |
| D | BsiHKA I | GWGCW/C |
| D | Bsl I | CCNNNNN/NNGG |
| D | BssK I | /CCNGG |
| D | BstAP I | GCANNNN/NTGC |
| D | BstE II | G/GTNACC |
| D | BstN I | CC/WGG |
| D | BstX I | CCANNNNN/NTGG |
| D | Bsu36 I | CC/TNAGG |
| X | BthC I | GCNG/C |
| D | Cac8 I | GCN/NGC |
| D | Dde I | C/TNAG |
| D | Dra III | CACNNN/GTG |
| D | Drd I | GACNNNN/NNGTC |
| X | EcoH I | /CCSGG |
| D | EcoN I | CCTNN/NNNAGG |
| D | EcoO109 I | RG/GNCCY |
| Fal I | (8/13)AAGNNNNNCTT(13/8) | |
| X | Fmu I | GGNC/C |
| D | Fnu4H I | GC/NGC |
| X | Hae I | WGG/CCW |
| X | Hae IV | (7/13)GAYNNNNNRTC(14/9) |
| X | HgiE II | ACCNNNNNNGGT |
| D | Hinf I | G/ANTC |
| Hpy8 I | GTN/NAC | |
| D | Hpy99 I | CGWCG/ |
| D | Hpy188 I | TCN/GA |
| D | Hpy188 III | TC/NNGA |
| D | HpyCH4 III | ACN/GT |
| Mae III | /GTNAC | |
| X | Mja IV | GTNNAC |
| D | Msl I | CAYNN/NNRTG |
| D | Mwo I | GCNNNNN/NNGC |
| D | Nci I | CC/SGG |
| D | Nla IV | GGN/NCC |
| X | Pas I | CC/CWGGG |
| D | PflF I | GACN/NNGTC |
| D | PflM I | CCANNNN/NTGG |
| Pfo I | T/CCNGGA | |
| D | PpuM I | RG/GWCCY |
| D | PshA I | GACNN/NNGTC |
| X | Psp03 I | GGWC/C |
| D | PspG I | /CCWGG |
| X | Pss I | RGGNC/CY |
| D | Rsr II | CG/GWCCG |
| SanD I | GG/GWCCC | |
| D | Sau96 I | G/GNCC |
| D | ScrF I | CC/NGG |
| D | SexA I | A/CCWGGT |
| D | Sfi I | GGCCNNNN/NGGCC |
| X | Sse8647 I | AG/GWCCT |
| D | Sty I | C/CWWGG |
| D | StyD4 I | /CCNGG |
| Tat I | W/GTACW | |
| Tau I | GCSG/C | |
| D | Tfi I | G/AWTC |
| D | Tse I | G/CWGC |
| D | Tsp45 I | /GTSAC |
| D | TspR I | CASTGNN/ |
| D | Tth111 I | GACN/NNGTC |
| X | UbaM I | TCCNGGA |
| X | Unb I | /GGNCC |
| X | VpaK11A I | /GGWCC |
| D | Xcm I | CCANNNNN/NNNNTGG |
| D | Xmn I | GAANN/NNTTC |
Note:
Enzymes marked with an ( D ) are available
from NEB.
Enzymes marked with an ( X ) are not currently commercially available