!!NA_SEQUENCE 1.0 Plasmid LITMUS 28i Update 5/24/02 Features: 143-1003 beta-lactamase (bla; amp-r) CDS (start 143) 143- 211 beta-lactamase signal peptide CDS (start 143) 1045-1554 M13 origin of replication (+ -) 1665-2253 pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point) 2424-2795 lacZ alpha CDS (start 2424) 2449-2466 T7 promoter (transcript start 2466 clockwise) 2470-2589 multiple cloning site (BglII-KpnI) 2620-2603 T7 promoter (transcript start 2603 counter-clockwise) litmus28i.seq Length: 2823 May 24, 2002 12:01 Type: N Check: 9632 .. 1 GTTAACTACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 51 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 101 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 151 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 201 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 251 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 301 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTCTCCAATG ATGAGCACTT 351 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA 401 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 451 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 501 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 551 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 601 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 651 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 701 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 751 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 801 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 851 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 901 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 951 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 1001 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTACCCCG 1051 GTTGATAATC AGAAAAGCCC CAAAAACAGG AAGATTGTAT AAGCAAATAT 1101 TTAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT 1151 AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT 1201 AAATCAAAAG AATAGCCCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA 1251 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA 1301 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CAAATCAAGT 1351 TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 1401 CCCCCGATTT AGAGCTTGAC GGGGAAAGCG AACGTGGCGA GAAAGGAAGG 1451 GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA 1501 CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC 1551 GCGTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 1601 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 1651 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 1701 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 1751 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA 1801 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 1851 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 1901 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 1951 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 2001 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 2051 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG 2101 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 2151 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 2201 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 2251 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 2301 TTTTGCTCAC ATGTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 2351 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 2401 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTACGTA 2451 ATACGACTCA CTATAGGGCA GATCTTCGAA TGCATCGCGC GCACCGTACG 2501 TCTCGAGGAA TTCCTGCAGG ATATCTGGAT CCACGAAGCT TCCCATGGTG 2551 ACGTCACCGG TTCTAGATAC CTAGGTGAGC TCTGGTACCC TCTAGTCAAG 2601 GCCTATAGTG AGTCGTATTA CGGACTGGCC GTCGTTTTAC AACGTCGTGA 2651 CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 2701 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC 2751 CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTCGCTT GGTAATAAAG 2801 CCCGCTTCGG CGGGCTTTTT TTT