!!NA_SEQUENCE 1.0 Plasmid pFOS1 Update 5/24/02 Features: 134- 994 beta-lactamase (bla; amp-r) CDS (start 134) 134- 202 beta-lactamase signal peptide CDS (start 134) 1165-1753 pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point) 2024-2422 lambda cos site 2440-2473 loxP site 2489-2506 T7 promoter (transcript start 2489 clockwise) 2539-2523 SP6 promoter (transcript counterclockwise) 3338-2679 chloramphenicol resistance CDS (cat) (start 3338, complementary strand) 4675-5430 repE (repA) (start 4675) 4280-4346 oriS (ori2) 6009-7184 sopA (parA) (start 6009) 7184-8155 sopB (parB) (start 7184) 8228-8701 sopC (parC, incD) (start 8228) 8960-9360 lambda cos site pfos1.seq Length: 9738 May 24, 2002 16:07 Type: N Check: 4520 .. 1 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 51 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA 101 TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT 151 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 201 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 251 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA 301 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 351 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC 401 GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT 451 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 501 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 551 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA 601 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 651 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC 701 AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT 751 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 801 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 851 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT 901 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 951 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA 1001 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 1051 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 1101 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 1151 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 1201 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 1251 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA 1301 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 1351 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 1401 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 1451 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 1501 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 1551 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG 1601 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 1651 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 1701 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 1751 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 1801 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 1851 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC 1901 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 1951 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TTGCGCCGTT 2001 CCACTTCGAT GCGTCAGTGA AGCGACATGA GGTTGCCCCG TATTCAGTGT 2051 CGCTGATTTG TATTGTCTGA AGTTGTTTTT ACGTTAAGTT GATGCAGATC 2101 AATTAATACG ATACCTGCGT CATAATTGAT TATTTGACGT GGTTTGATGG 2151 CCTCCACGCA CGTTGTGATA TGTAGATGAT AATCATTATC ACTTTACGGG 2201 TCCTTTCCGG TGATCCGACA GGTTACGGGG CGGCGACCTC GCGGGTTTTC 2251 GCTATTTATG AAAATTTTCC GGTTTAAGGC GTTTCCGTTC TTCTTCGTCA 2301 TAACTTAATG TTTTTATTTA AAATACCCTC TGAAAAGAAA GGAAACGACA 2351 GGTGCTGAAA GCGAGCTTTT TGGCCTCTGT CGTTTCCTTT CTCTGTTTTT 2401 GTCCGTGGAA TGAACAATGG AAGTCCGAGC TCATCGCTAA TAACTTCGTA 2451 TAGCATACAT TATACGAAGT TATATTCGAT GCGGCCGCTA ATACGACTCA 2501 CTATAGGGAG AAGCTTGGAT CCTATAGTGT CACCTAAATC GTATGCGGCC 2551 GCCCGGGCCG TCGACCAATT CTCATGTTTG ACAGCTTATC ATCGAATTTC 2601 TGCCATTCAT CCGCTTATTA TCACTTATTC AGGCGTAGCA CCAGGCGTTT 2651 AAGGGCACCA ATAACTGCCT TAAAAAAATT ACGCCCCGCC CTGCCACTCA 2701 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC 2751 ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC 2801 CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 2851 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC 2901 TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT 2951 TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 3001 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC 3051 ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC 3101 CGTCTTTCAT TGCCATACGG AATTCCGGAT GAGCATTCAT CAGGCGGGCA 3151 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT 3201 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT 3251 GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 3301 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT TAGCTTCCTT 3351 AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA 3401 TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT 3451 TTTCGCCAAA AGTTGGCCCA GGGCTTCCCG GTATCAACAG GGACACCAGG 3501 ATTTATTTAT TCTGCGAAGT GATCTTCCGT CACAGGTATT TATTCGCGAT 3551 AAGCTCATGG AGCGGCGTAA CCGTCGCACA GGAAGGACAG AGAAAGCGCG 3601 GATCTGGGAA GTGACGGACA GAACGGTCAG GACCTGGATT GGGGAGGCGG 3651 TTGCCGCCGC TGCTGCTGAC GGTGTGACGT TCTCTGTTCC GGTCACACCA 3701 CATACGTTCC GCCATTCCTA TGCGATGCAC ATGCTGTATG CCGGTATACC 3751 GCTGAAAGTT CTGCAAAGCC TGATGGGACA TAAGTCCATC AGTTCAACGG 3801 AAGTCTACAC GAAGGTTTTT GCGCTGGATG TGGCTGCCCG GCACCGGGTG 3851 CAGTTTGCGA TGCCGGAGTC TGATGCGGTT GCGATGCTGA AACAATTATC 3901 CTGAGAATAA ATGCCTTGGC CTTTATATGG AAATGTGGAA CTGAGTGGAT 3951 ATGCTGTTTT TGTCTGTTAA ACAGAGAAGC TGGCTGTTAT CCACTGAGAA 4001 GCGAACGAAA CAGTCGGGAA AATCTCCCAT TATCGTAGAG ATCCGCATTA 4051 TTAATCTCAG GAGCCTGTGT AGCGTTTATA GGAAGTAGTG TTCTGTCATG 4101 ATGCCTGCAA GCGGTAACGA AAACGATTTG AATATGCCTT CAGGAACAAT 4151 AGAAATCTTC GTGCGGTGTT ACGTTGAAGT GGAGCGGATT ATGTCAGCAA 4201 TGGACAGAAC AACCTAATGA ACACAGAACC ATGATGTGGT CTGTCCTTTT 4251 ACAGCCAGTA GGCTCGCCGC AGTCGAGCGA CGGCGAAGCC CTCGAGTGAG 4301 CGAGGAAGCA CCAGGGAACA GCACTTATAT ATTCTGCTTA CACACGATGC 4351 CTGAAAAAAC TTCCCTTGGG GTTATCCACT TATCCACGGG GATATTTTTA 4401 TAATTATTTT TTTTATAGTT TTTAGATCTT CTTTTTTAGA GCGCCTTGTA 4451 GGCCTTTATC CATGCTGGTT CTAGAGAAGG TGTTGTGACA AATTGCCCTT 4501 TCAGTGTGAC AAATCACCCT CAAATGACAG TCCTGTCTGT GACAAATTGC 4551 CCTTAACCCT GTGACAAATT GCCCTCAGAA GAAGCTGTTT TTTCACAAAG 4601 TTATCCCTGC TTATTGACTC TTTTTTATTT AGTGTGACAA TCTAAAAACT 4651 TGTCACACTT CACATGGATC TGTCATGGCG GAAACAGCGG TTATCAATCA 4701 CAAGAAACGT AAAAATAGCC CGCGAATCGT CCAGTCAAAC GACCTCACTG 4751 AGGCGGCATA TAGTCTCTCC CGGGATCAAA AACGTATGCT GTATCTGTTC 4801 GTTGACCAGA TCAGAAAATC TGATGGCACC CTACAGGAAC ATGACGGTAT 4851 CTGCGAGATC CATGTTGCTA AATATGCTGA AATATTCGGA TTGACCTCTG 4901 CGGAAGCCAG TAAGGATATA CGGCAGGCAT TGAAGAGTTT CGCGGGGAAG 4951 GAAGTGGTTT TTTATCGCCC TGAAGAGGAT GCCGGCGATG AAAAAGGCTA 5001 TGAATCTTTT CCTTGGTTTA TCAAACGTGC GCACAGTCCA TCCAGAGGGC 5051 TTTACAGTGT ACATATCAAC CCATATCTCA TTCCCTTCTT TATCGGGTTA 5101 CAGAACCGGT TTACGCAGTT TCGGCTTAGT GAAACAAAAG AAATCACCAA 5151 TCCGTATGCC ATGCGTTTAT ACGAATCCCT GTGTCAGTAT CGTAAGCCGG 5201 ATGGCTCAGG CATCGTCTCT CTGAAAATCG ACTGGATCAT AGAGCGTTAC 5251 CAGCTGCCTC AAAGTTACCA GCGTATGCCT GACTTCCGCC GCCGCTTCCT 5301 GCAGGTCTGT GTTAATGAGA TCAACAGCAG AACTCCAATG CGCCTCTCAT 5351 ACATTGAGAA AAAGAAAGGC CGCCAGACGA CTCATATCGT ATTTTCCTTC 5401 CGCGATATCA CTTCCATGAC GACAGGATAG TCTGAGGGTT ATCTGTCACA 5451 GATTTGAGGG TGGTTCGTCA CATTTGTTCT GACCTACTGA GGGTAATTTG 5501 TCACAGTTTT GCTGTTTCCT TCAGCCTGCA TGGATTTTCT CATACTTTTT 5551 GAACTGTAAT TTTTAAGGAA GCCAAATTTG AGGGCAGTTT GTCACAGTTG 5601 ATTTCCTTCT CTTTCCCTTC GTCATGTGAC CTGATATCGG GGGTTAGTTC 5651 GTCATCATTG ATGAGGGTTG ATTATCACAG TTTATTACTC TGAATTGGCT 5701 ATCCGCGTGT GTACCTCTAC CTGGAGTTTT TCCCACGGTG GATATTTCTT 5751 CTTGCGCTGA GCGTAAGAGC TATCTGACAG AACAGTTCTT CTTTGCTTCC 5801 TCGCCAGTTC GCTCGCTATG CTCGGTTACA CGGCTGCGGC GAGCGCTAGT 5851 GATAATAAGT GACTGAGGTA TGTGCTCTTC TTATCTCCTT TTGTAGTGTT 5901 GCTCTTATTT TAAACAACTT TGCGGTTTTT TGATGACTTT GCGATTTTGT 5951 TGTTGCTTTG CAGTAAATTG CAAGATTTAA TAAAAAAACG CAAAGCAATG 6001 ATTAAAGGAT GTTCAGAATG AAACTCATGG AAACACTTAA CCAGTGCATA 6051 AACGCTGGTC ATGAAATGAC GAAGGCTATC GCCATTGCAC AGTTTAATGA 6101 TGACAGCCCG GAAGCGAGGA AAATAACCCG GCGCTGGAGA ATAGGTGAAG 6151 CAGCGGATTT AGTTGGGGTT TCTTCTCAGG CTATCAGAGA TGCCGAGAAA 6201 GCAGGGCGAC TACCGCACCC GGATATGGAA ATTCGAGGAC GGGTTGAGCA 6251 ACGTGTTGGT TATACAATTG AACAAATTAA TCATATGCGT GATGTGTTTG 6301 GTACGCGATT GCGACGTGCT GAAGACGTAT TTCCACCGGT GATCGGGGTT 6351 GCTGCCCATA AAGGTGGCGT TTACAAAACC TCAGTTTCTG TTCATCTTGC 6401 TCAGGATCTG GCTCTGAAGG GGCTACGTGT TTTGCTCGTG GAAGGTAACG 6451 ACCCCCAGGG AACAGCCTCA ATGTATCACG GATGGGTACC AGATCTTCAT 6501 ATTCATGCAG AAGACACTCT CCTGCCTTTC TATCTTGGGG AAAAGGACGA 6551 TGTCACTTAT GCAATAAAGC CCACTTGCTG GCCGGGGCTT GACATTATTC 6601 CTTCCTGTCT GGCTCTGCAC CGTATTGAAA CTGAGTTAAT GGGCAAATTT 6651 GATGAAGGTA AACTGCCCAC CGATCCACAC CTGATGCTCC GACTGGCCAT 6701 TGAAACTGTT GCTCATGACT ATGATGTCAT AGTTATTGAC AGCGCGCCTA 6751 ACCTGGGTAT CGGCACGATT AATGTCGTAT GTGCTGCTGA TGTGCTGATT 6801 GTTCCCACGC CTGCTGAGTT GTTTGACTAC ACCTCCGCAC TGCAGTTTTT 6851 CGATATGCTT CGTGATCTGC TCAAGAACGT TGATCTTAAA GGGTTCGAGC 6901 CTGATGTACG TATTTTGCTT ACCAAATACA GCAATAGTAA TGGCTCTCAG 6951 TCCCCGTGGA TGGAGGAGCA AATTCGGGAT GCCTGGGGAA GCATGGTTCT 7001 AAAAAATGTT GTACGTGAAA CGGATGAAGT TGGTAAAGGT CAGATCCGGA 7051 TGAGAACTGT TTTTGAACAG GCCATTGATC AACGCTCTTC AACTGGTGCC 7101 TGGAGAAATG CTCTTTCTAT TTGGGAACCT GTCTGCAATG AAATTTTCGA 7151 TCGTCTGATT AAACCACGCT GGGAGATTAG ATAATGAAGC GTGCGCCTGT 7201 TATTCCAAAA CATACGCTCA ATACTCAACC GGTTGAAGAT ACTTCGTTAT 7251 CGACACCAGC TGCCCCGATG GTGGATTCGT TAATTGCGCG CGTAGGAGTA 7301 ATGGCTCGCG GTAATGCCAT TACTTTGCCT GTATGTGGTC GGGATGTGAA 7351 GTTTACTCTT GAAGTGCTCC GGGGTGATAG TGTTGAGAAG ACCTCTCGGG 7401 TATGGTCAGG TAATGAACGT GACCAGGAGC TGCTTACTGA GGACGCACTG 7451 GATGATCTCA TCCCTTCTTT TCTACTGACT GGTCAACAGA CACCGGCGTT 7501 CGGTCGAAGA GTATCTGGTG TCATAGAAAT TGCCGATGGG AGTCGCCGTC 7551 GTAAAGCTGC TGCACTTACC GAAAGTGATT ATCGTGTTCT GGTTGGCGAG 7601 CTGGATGATG AGCAGATGGC TGCATTATCC AGATTGGGTA ACGATTATCG 7651 CCCAACAAGT GCTTATGAAC GTGGTCAGCG TTATGCAAGC CGATTGCAGA 7701 ATGAATTTGC TGGAAATATT TCTGCGCTGG CTGATGCGGA AAATATTTCA 7751 CGTAAGATTA TTACCCGCTG TATCAACACC GCCAAATTGC CTAAATCAGT 7801 TGTTGCTCTT TTTTCTCACC CCGGTGAACT ATCTGCCCGG TCAGGTGATG 7851 CACTTCAAAA AGCCTTTACA GATAAAGAGG AATTACTTAA GCAGCAGGCA 7901 TCTAACCTTC ATGAGCAGAA AAAAGCTGGG GTGATATTTG AAGCTGAAGA 7951 AGTTATCACT CTTTTAACTT CTGTGCTTAA AACGTCATCT GCATCAAGAA 8001 CTAGTTTAAG CTCACGACAT CAGTTTGCTC CTGGAGCGAC AGTATTGTAT 8051 AAGGGCGATA AAATGGTGCT TAACCTGGAC AGGTCTCGTG TTCCAACTGA 8101 GTGTATAGAG AAAATTGAGG CCATTCTTAA GGAACTTGAA AAGCCAGCAC 8151 CCTGATGCGA CCACGTTTTA GTCTACGTTT ATCTGTCTTT ACTTAATGTC 8201 CTTTGTTACA GGCCAGAAAG CATAACTGGC CTGAATATTC TCTCTGGGCC 8251 CACTGTTCCA CTTGTATCGT CGGTCTGATA ATCAGACTGG GACCACGGTC 8301 CCACTCGTAT CGTCGGTCTG ATTATTAGTC TGGGACCACG GTCCCACTCG 8351 TATCGTCGGT CTGATTATTA GTCTGGGACC ACGGTCCCAC TCGTATCGTC 8401 GGTCTGATAA TCAGACTGGG ACCACGGTCC CACTCGTATC GTCGGTCTGA 8451 TTATTAGTCT GGGACCATGG TCCCACTCGT ATCGTCGGTC TGATTATTAG 8501 TCTGGGACCA CGGTCCCACT CGTATCGTCG GTCTGATTAT TAGTCTGGAA 8551 CCACGGTCCC ACTCGTATCG TCGGTCTGAT TATTAGTCTG GGACCACGGT 8601 CCCACTCGTA TCGTCGGTCT GATTATTAGT CTGGGACCAC GATCCCACTC 8651 GTGTTGTCGG TCTGATTATC GGTCTGGGAC CACGGTCCCA CTTGTATTGT 8701 CGATCAGACT ATCAGCGTGA GACTACGATT CCATCAATGC CTGTCAAGGG 8751 CAAGTATTGA CATGTCGTCG TAACCTGTAG AACGGAGTAA CCTCGGTGTG 8801 CGGTTGTATG CCTGCTGTGG ATTGCTGCTG TGTCCTGCTT ATCCACAACA 8851 TTTTGCGCAC GGTTATGTGG ACAAAATACC TGGTTACCCA GGCCGTGCCG 8901 GCACGTTAAC CGGGCTGCAT CCGATGCAAG TGTGTCGCTG TCGACGAGCT 8951 CGCGAGCTCG GACATGAGGT TGCCCCGTAT TCAGTGTCGC TGATTTGTAT 9001 TGTCTGAAGT TGTTTTTACG TTAAGTTGAT GCAGATCAAT TAATACGATA 9051 CCTGCGTCAT AATTGATTAT TTGACGTGGT TTGATGGCCT CCACGCACGT 9101 TGTGATATGT AGATGATAAT CATTATCACT TTACGGGTCC TTTCCGGTGA 9151 TCCGACAGGT TACGGGGCGG CGACCTCGCG GGTTTTCGCT ATTTATGAAA 9201 ATTTTCCGGT TTAAGGCGTT TCCGTTCTTC TTCGTCATAA CTTAATGTTT 9251 TTATTTAAAA TACCCTCTGA AAAGAAAGGA AACGACAGGT GCTGAAAGCG 9301 AGGCTTTTTG GCCTCTGTCG TTTCCTTTCT CTGTTTTTGT CCGTGGAATG 9351 AACAATGGAA GTCCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT 9401 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT 9451 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA 9501 GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA 9551 ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA 9601 CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC 9651 CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT 9701 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGAC