!!NA_SEQUENCE 1.0 Plasmid pACYC184 Update 6/6/02 Features: 219-3805 chloramphenicol resistance (cat) CDS (start 219, complementary strand) 291- 286 cat promoter -10 sequence (CATAAT) 314- 309 cat promoter -35 sequence (ACGTAA) 1393- 848 p15A origin of replication (counterclockwise) (RNAII -35 to RNA/DNA switch point) 1505-1510 tet promoter -35 sequence (TTGACA) 1528-1533 tet promoter -10 sequence (TTTAAT) 1540 tet RNA transcript start 1581-2771 tetracycline resistance (tet) CDS (start 1581) pacyc184.seq Length: 4245 June 6, 2002 16:06 Type: N Check: 8387 .. 1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 51 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 101 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC 151 CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 201 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT 251 AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT 301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC 351 AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG 401 TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC 451 TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG 501 GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT 551 GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT 601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG 651 CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA 701 TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG 751 CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC 801 CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT 851 CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC 901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 951 GGCGGCTCCC TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT 1001 CATTCCGCTG TTATGGCCGC GTTTGTCTCA TTCCACGCCT GACACTCAGT 1051 TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC TGTATGCACG AACCCCCCGT 1101 TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 1151 CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT 1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA 1251 CAAGTTTTGG TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG 1301 TTGGTAGCTC AGAGAACCTT CGAAAAACCG CCCTGCAAGG CGGTTTTTTC 1351 GTTTTCAGAG CAAGAGATTA CGCGCAGACC AAAACGATCT CAAGAAGATC 1401 ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA ATTTATCTCT 1451 TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC 1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG 1551 TTAAATTGCT AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT 1601 CATCGTCATC CTCGGCACCG TCACCCTGGA TGCTGTAGGC ATAGGCTTGG 1651 TTATGCCGGT ACTGCCGGGC CTCTTGCGGG ATATCGTCCA TTCCGACAGC 1701 ATCGCCAGTC ACTATGGCGT GCTGCTAGCG CTATATGCGT TGATGCAATT 1751 TCTATGCGCA CCCGTTCTCG GAGCACTGTC CGACCGCTTT GGCCGCCGCC 1801 CAGTCCTGCT CGCTTCGCTA CTTGGAGCCA CTATCGACTA CGCGATCATG 1851 GCGACCACAC CCGTCCTGTG GATCCTCTAC GCCGGACGCA TCGTGGCCGG 1901 CATCACCGGC GCCACAGGTG CGGTTGCTGG CGCCTATATC GCCGACATCA 1951 CCGATGGGGA AGATCGGGCT CGCCACTTCG GGCTCATGAG CGCTTGTTTC 2001 GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC GGGGGACTGT TGGGCGCCAT 2051 CTCCTTGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC 2101 TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA 2151 CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG 2201 GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC 2251 TCGTAGGACA GGTGCCGGCA GCGCTCTGGG TCATTTTCGG CGAGGACCGC 2301 TTTCGCTGGA GCGCGACGAT GATCGGCCTG TCGCTTGCGG TATTCGGAAT 2351 CTTGCACGCC CTCGCTCAAG CCTTCGTCAC TGGTCCCGCC ACCAAACGTT 2401 TCGGCGAGAA GCAGGCCATT ATCGCCGGCA TGGCGGCCGA CGCGCTGGGC 2451 TACGTCTTGC TGGCGTTCGC GACGCGAGGC TGGATGGCCT TCCCCATTAT 2501 GATTCTTCTC GCTTCCGGCG GCATCGGGAT GCCCGCGTTG CAGGCCATGC 2551 TGTCCAGGCA GGTAGATGAC GACCATCAGG GACAGCTTCA AGGATCGCTC 2601 GCGGCTCTTA CCAGCCTAAC TTCGATCACT GGACCGCTGA TCGTCACGGC 2651 GATTTATGCC GCCTCGGCGA GCACATGGAA CGGGTTGGCA TGGATTGTAG 2701 GCGCCGCCCT ATACCTTGTC TGCCTCCCCG CGTTGCGTCG CGGTGCATGG 2751 AGCCGGGCCA CCTCGACCTG AATGGAAGCC GGCGGCACCT CGCTAACGGA 2801 TTCACCACTC CAAGAATTGG AGCCAATCAA TTCTTGCGGA GAACTGTGAA 2851 TGCGCAAACC AACCCTTGGC AGAACATATC CATCGCGTCC GCCATCTCCA 2901 GCAGCCGCAC GCGGCGCATC TCGGGCAGCG TTGGGTCCTG GCCACGGGTG 2951 CGCATGATCG TGCTCCTGTC GTTGAGGACC CGGCTAGGCT GGCGGGGTTG 3001 CCTTACTGGT TAGCAGAATG AATCACCGAT ACGCGAGCGA ACGTGAAGCG 3051 ACTGCTGCTG CAAAACGTCT GCGACCTGAG CAACAACATG AATGGTCTTC 3101 GGTTTCCGTG TTTCGTAAAG TCTGGAAACG CGGAAGTCCC CTACGTGCTG 3151 CTGAAGTTGC CCGCAACAGA GAGTGGAACC AACCGGTGAT ACCACGATAC 3201 TATGACTGAG AGTCAACGCC ATGAGCGGCC TCATTTCTTA TTCTGAGTTA 3251 CAACAGTCCG CACCGCTGTC CGGTAGCTCC TTCCGGTGGG CGCGGGGCAT 3301 GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG 3351 GACAGGTGCC GGCAGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC 3401 ATACCCACGC CGAAACAAGC GCCCTGCACC ATTATGTTCC GGATCTGCAT 3451 CGCAGGATGC TGCTGGCTAC CCTGTGGAAC ACCTACATCT GTATTAACGA 3501 AGCGCTAACC GTTTTTATCA GGCTCTGGGA GGCAGAATAA ATGATCATAT 3551 CGTCAATTAT TACCTCCACG GGGAGAGCCT GAGCAAACTG GCCTCAGGCA 3601 TTTGAGAAGC ACACGGTCAC ACTGCTTCCG GTAGTCAATA AACCGGTAAA 3651 CCAGCAATAG ACATAAGCGG CTATTTAACG ACCCTGCCCT GAACCGACGA 3701 CCGGGTCGAA TTTGCTTTCG AATTTCTGCC ATTCATCCGC TTATTATCAC 3751 TTATTCAGGC GTAGCACCAG GCGTTTAAGG GCACCAATAA CTGCCTTAAA 3801 AAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA 3851 AGCATTCTGC CGACATGGAA GCCATCACAG ACGGCATGAT GAACCTGAAT 3901 CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG 3951 TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 4001 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT 4051 AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT 4101 GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG 4151 AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG 4201 AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACG