!!NA_SEQUENCE 1.0 422-1234 aminoglycoside 3'-phosphotransferase (Kn) 1965-2477 M13 origin 3177 ColE1 origin 3549-3737 rop 4297-5376 lacI 6560-6577 T7 promoter 6560-6579 T7 universal primer (forward) 6577 first nucleotide of the T7 transcript 6580-6604 lac operator 6633-6639 Shine-Dalgarno sequence (T7 gene 10) 6646-6698 Multiple cloning site 6699-8060 Sce VMA intein 6762-6785 Intein reverse sequencing primer 8094-8249 Chitin-binding domain 8338-8375 T7 transcription terminator KYB1Jan3100.seq Length: 8393 January 31, 2000 18:23 Type: N Check: 5735 .. 1 TCCGGTGCAG CCTTTTTTCT CCTGCCACAT GAAGCACTTC ACTGACACCC 51 TCATCAGTGC CAACATAGTA AGCCAGTATA CACTCCGCTA GCGCTGAGGT 101 CTGCCTCGTG AAGAAGGTGT TGCTGACTCA TACCAGGCCT GAATCGCCCC 151 ATCATCCAGC CAGAAAGTGA GGGAGCCACG GTTGATGAGA GCTTTGTTGT 201 AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT 251 GCGTTGTCGG GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG 301 ATTTATTCAA CAAAGCCACG TTGTGTCTCA AAATCTCTGA TGTTACATTG 351 CACAAGATAA AAATATATCA TCATGAACAA TAAAACTGTC TGCTTACATA 401 AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCTTG 451 CTCGCGTCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA 501 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGATTG 551 TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 601 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG 651 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT 701 GCATGGTTAC TCACCACTGC GATCCCCGGC AAAACAGCAT TCCAGGTATT 751 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT 801 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC 851 GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 901 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC 951 AAGTCTGGAA AGAAATGCAT AAGCTTTTGC CATTCTCACC GGATTCAGTC 1001 GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA 1051 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC 1101 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA 1151 CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 1201 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA 1251 ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGGC 1301 TTTGTTGAGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 1351 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 1401 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 1451 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 1501 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 1551 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 1601 TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 1651 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 1701 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 1751 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 1801 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 1851 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 1901 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 1951 CTTTAGATTG ATTTACCCCG GTTGATAATC AGAAAAGCCC CAAAAACAGG 2001 AAGATTGTAT AAGCAAATAT TTAAATTGTA AACGTTAATA TTTTGTTAAA 2051 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG 2101 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGCCCGA GATAGGGTTG 2151 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 2201 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG 2251 AACCATCACC CAAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA 2301 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC 2351 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA 2401 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC 2451 GCGCTTAATG CGCCGCTACA GGGCGCGTAA AAGGATCTAG GTGAAGATCC 2501 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 2551 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 2601 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 2651 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 2701 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 2751 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG 2801 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 2851 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 2901 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 2951 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT 3001 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3051 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA 3101 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 3151 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3201 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 3251 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 3301 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 3351 AGCTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 3401 CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA 3451 CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC 3501 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA 3551 GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA 3601 TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC 3651 CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC 3701 GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG 3751 TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG 3801 GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC 3851 GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA 3901 ATCACTCAGG GTCAATGCCA GCCGAACGCC AGCAAGACGT AGCCCAGCGC 3951 GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG 4001 TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT 4051 ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC 4101 GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA 4151 TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC 4201 CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC 4251 CGGTGCCTAA TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC 4301 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC 4351 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG GTGGTTTTTC 4401 TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC 4451 TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA 4501 ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG 4551 TATCGTCGTA TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC 4601 TCGGTAATGG CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG 4651 CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG GTTTGTTGAA 4701 AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT 4751 TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA 4801 GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG 4851 CGACCAGATG CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA 4901 ATACTGTTGA TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC 4951 ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA TCCAGCGGAT 5001 AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC 5051 GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT 5101 GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG 5151 GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT 5201 TTGCCCGCCA GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC 5251 CATCGCCGCT TCCACTTTTT CCCGCGTTTT CGCAGAAACG TGGCTGGCCT 5301 GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC ATACTCTGCG 5351 ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC 5401 TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG 5451 TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA 5501 GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG 5551 CATGCCGGCA TGCCGCCCTT TCGTCTTCAA GAATTAATTC CCAATTCCCC 5601 AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT 5651 TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGG 5701 GAGCGGATTT GAACGTTGCG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA 5751 CGCCCGCCAT AAACTGCCAG GAATTAATTC CCCAGGCATC AAATAAAACG 5801 AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG 5851 TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGGGAGCGGA TTTGAACGTT 5901 GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC CATAAACTGC 5951 CAGGAATTAA TTCCCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG 6001 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT 6051 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG 6101 AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGAAT TAATTCCCCA 6151 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT 6201 ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG 6251 AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC 6301 GCCCGCCATA AACTGCCAGG AATTAATTCC CCAGGCATCA AATAAAACGA 6351 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT 6401 GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG 6451 CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC 6501 AGGAATTGGG GATCGGAATT AATTCCCGGT TTAAACCGGG GATCTCGATC 6551 CCGCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT 6601 TCCCCTCTAG AAATAATTTT GTTTAACTTT AAGAAGGAGA TATAcatatg 6651 gctagctcgc gagtcgacgg cggccgcgaa ttcctcgagg gctcttccTG 6701 CTTTGCCAAG GGTACCAATG TTTTAATGGC GGATGGGTCT ATTGAATGTA 6751 TTGAAAACAT TGAGGTTGGT AATAAGGTCA TGGGTAAAGA TGGCAGACCT 6801 CGTGAGGTAA TTAAATTGCC CAGAGGAAGA GAAACTATGT ACAGCGTCGT 6851 GCAGAAAAGT CAGCACAGAG CCCACAAAAG TGACTCAAGT CGTGAAGTGC 6901 CAGAATTACT CAAGTTTACG TGTAATGCGA CCCATGAGTT GGTTGTTAGA 6951 ACACCTCGTA GTGTCCGCCG TTTGTCTCGT ACCATTAAGG GTGTCGAATA 7001 TTTTGAAGTT ATTACTTTTG AGATGGGCCA AAAGAAAGCC CCCGACGGTA 7051 GAATTGTTGA GCTTGTCAAG GAAGTTTCAA AGAGCTACCC AATATCTGAG 7101 GGGCCTGAGA GAGCCAACGA ATTAGTAGAA TCCTATAGAA AGGCTTCAAA 7151 TAAAGCTTAT TTTGAGTGGA CTATTGAGGC CAGAGATCTT TCTCTGTTGG 7201 GTTCCCATGT TCGTAAAGCT ACCTACCAGA CTTACGCTCC AATTCTTTAT 7251 GAGAATGACC ACTTTTTCGA CTACATGCAA AAAAGTAAGT TTCATCTCAC 7301 CATTGAAGGT CCAAAAGTAC TTGCTTATTT ACTTGGTTTA TGGATTGGTG 7351 ATGGATTGTC TGACAGGGCA ACTTTTTCGG TTGATTCCAG AGATACTTCT 7401 TTGATGGAAC GTGTTACTGA ATATGCTGAA AAGTTGAATT TGTGCGCCGA 7451 GTATAAGGAC AGAAAAGAAC CACAAGTTGC CAAAACTGTT AATTTGTACT 7501 CTAAAGTTGT CAGAGGTAAT GGTATTCGCA ATAATCTTAA TACTGAGAAT 7551 CCATTATGGG ACGCTATTGT TGGCTTAGGA TTCTTGAAGG ACGGTGTCAA 7601 AAATATTCCT TCTTTCTTGT CTACGGACAA TATCGGTACT CGTGAAACAT 7651 TTCTTGCTGG TCTAATTGAT TCTGATGGCT ATGTTACTGA TGAGCATGGT 7701 ATTAAAGCAA CAATAAAGAC AATTCATACT TCTGTCAGAG ATGGTTTGGT 7751 TTCCCTTGCT CGTTCTTTAG GCTTAGTAGT CTCGGTTAAC GCAGAACCTG 7801 CTAAGGTTGA CATGAATGTC ACCAAACATA AAATTAGTTA TGCTATTTAT 7851 ATGTCTGGTG GAGATGTTTT GCTTAACGTT CTTTCGAAGT GTGCCGGCTC 7901 TAAAAAATTC AGGCCTGCTC CCGCCGCTGC TTTTGCACGT GAGTGCCGCG 7951 GATTTTATTT CGAGTTACAA GAATTGAAGG AAGACGATTA TTATGGGATT 8001 ACTTTATCTG ATGATTCTGA TCATCAGTTT TTGCTTGGAT CCCAGGTTGT 8051 CGTCCATgca TGCGGTGGCC TGACCGGTCT GAACTCAGGC CTCACGACAA 8101 ATCCTGGTGT ATCCGCTTGG CAGGTCAACA CAGCTTATAC TGCGGGACAA 8151 TTGGTCACAT ATAACGGCAA GACGTATAAA TGTTTGCAGC CCCACACCTC 8201 CTTGGCAGGA TGGGAACCAT CCAACGTTCC TGCCTTGTGG CAGCTTCAAT 8251 GActgcagga aggGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG 8301 TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 8351 TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATA