!!NA_SEQUENCE 1.0 pMAL-c lacI-Ptac-malE(del2-26)-fx-lacZalpha bla Sequence compiled from known sequence of component fragments (Genbank) with confirmation of novel joints created in the construction. Revised 6/21/94; 1535-6 TC changed to CT Revised 2/9/00 Revised 3/27/00 Features: 15-46 lacI promoter 81 lacI start codon (GTG) 1161 lacI termination codon (TGA) 1205 Begin Ptac DNA fragment 1406-1433 Ptac promoter 1478 Begin malE DNA fragment 1528 malE start codon (ATG) 2587-2610 Sequence of malE primer #1237 2629 Start polylinker, factor Xa site 2732 Start lacZ-alpha sequence (CA CTG GCC) 2898 Start rrnB terminator DNA fragment 2911 First in-frame stop codon for lacZ-alpha 3101-3304 rrnB T1T2 3324 Start pBR322 sequence 3415 beta-lactamase (Amp-r) start codon (ATG) 4273 beta-lactamase stop codon (TAA) 4446-5034 pBR322 ori 5462 rop stop codon (TGA on bottom strand) 5651 rop start codon (GTG on bottom strand) 6146 End pmal-c.seq Length: 6146 July 28, 2000 11:01 Type: N Check: 5180 .. 1 CCGACACCAT CGAATGGCGC AAAACCTTTC GCGGTATGGC ATGATAGCGC 51 CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA 101 CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG 151 TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG 201 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC 251 GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC 301 ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG 351 GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG 401 TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA 451 TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC 501 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA 551 CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC 601 TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT 651 TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG 701 CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT 751 CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT 801 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT 851 TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG 901 ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA 951 CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT 1001 CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG 1051 TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC 1101 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 1151 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG 1201 GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT 1251 GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC 1301 GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT 1351 AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG 1401 AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG 1451 GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA 1501 GCACTTCACC AACAAGGACC ATAGATTATG AAAACTGAAG AAGGTAAACT 1551 GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG 1601 GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG 1651 GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC 1701 TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG 1751 GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT 1801 CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC 1851 GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA 1901 ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA 1951 GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC 2001 CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG 2051 GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG 2101 GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA 2151 CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA 2201 TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG 2251 AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC 2301 GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG 2351 AGCTGGCGAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG 2401 GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA 2451 CGAGGAAGAG TTGGCGAAAG ATCCACGTAT TGCCGCCACC ATGGAAAACG 2501 CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG 2551 TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT 2601 CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG GTACCCGGCC 2651 GGGGATCCAT CGAGGGTAGG CCTGAATTCA GTAAACCTAC CCTCGATGGA 2701 TCCTCTAGAG TCGACCTGCA GGCAAGCTTG GCACTGGCCG TCGTTTTACA 2751 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 2801 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT 2851 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC AGCTTGGCTG 2901 TTTTGGCGGA TGAGAGAAGA TTTTCAGCCT GATACAGATT AAATCAGAAC 2951 GCAGAAGCGG TCTGATAAAA CAGAATTTGC CTGGCGGCAG TAGCGCGGTG 3001 GTCCCACCTG ACCCCATGCC GAACTCAGAA GTGAAACGCC GTAGCGCCGA 3051 TGGTAGTGTG GGGTCTCCCC ATGCGAGAGT AGGGAACTGC CAGGCATCAA 3101 ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG 3151 TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG GGAGCGGATT 3201 TGAACGTTGC GAAGCAACGG CCCGGAGGGT GGCGGGCAGG ACGCCCGCCA 3251 TAAACTGCCA GGCATCAAAT TAAGCAGAAG GCCATCCTGA CGGATGGCCT 3301 TTTTGCGTTT CTACAAACTC TTTTTGTTTA TTTTTCTAAA TACATTCAAA 3351 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT 3401 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 3451 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT 3501 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 3551 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 3601 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 3651 ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT 3701 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 3751 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA 3801 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 3851 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 3901 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 3951 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC 4001 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 4051 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT 4101 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 4151 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 4201 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 4251 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA 4301 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 4351 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC 4401 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 4451 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 4501 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 4551 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 4601 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 4651 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 4701 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 4751 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 4801 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 4851 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 4901 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 4951 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 5001 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 5051 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 5101 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 5151 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 5201 GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG 5251 TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA 5301 TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT 5351 CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG 5401 CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG 5451 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA 5501 GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG 5551 CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC 5601 TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT 5651 CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC 5701 CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT 5751 GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG 5801 GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA 5851 CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC 5901 CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA 5951 AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT 6001 TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC 6051 TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG 6101 CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAAATT