!!NA_SEQUENCE 1.0 Plasmid pMAL-p2X Update 5/24/02 Features: 15- 46 lacIq promoter 81-1163 lacIq CDS (start 81) 1205 begin Ptac DNA fragment 1406-1433 Ptac promoter (clockwise) 1478 begin malE DNA fragment 1528-2991 expression CDS (start 1528) 1528-2703 malE sequence (start 1528) 1528-1605 malE signal sequence 2662-2685 sequence of malE primer (NEB #S1237S) 2704-2809 multiple cloning site sequence 2810-2991 lacZ alpha fragment sequence 2976 begin rrnB terminator fragment 3179-3382 rrnB T1T2 transcription terminators 3402 begin pBR322 fragment 3493-4353 beta-lactamase (bla; amp-r) CDS (start 3493) 3493-3561 beta-lactamase signal peptide CDS (start 3493) 4395-4908 M13 origin of replication (+ -) 5019-5607 pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point) 6228-6037 rop CDS (start 6228, complementary strand) pmalp2x.seq Length: 6721 May 24, 2002 16:35 Type: N Check: 8382 .. 1 CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC 51 CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA 101 CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG 151 TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG 201 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC 251 GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC 301 ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG 351 GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG 401 TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA 451 TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC 501 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA 551 CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC 601 TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT 651 TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG 701 CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT 751 CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT 801 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT 851 TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG 901 ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA 951 CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT 1001 CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG 1051 TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC 1101 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 1151 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG 1201 GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT 1251 GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC 1301 GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT 1351 AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG 1401 AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG 1451 GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA 1501 GCACTTCACC AACAAGGACC ATAGCATATG AAAATAAAAA CAGGTGCACG 1551 CATCCTCGCA TTATCCGCAT TAACGACGAT GATGTTTTCC GCCTCGGCTC 1601 TCGCCAAAAT CGAAGAAGGT AAACTGGTAA TCTGGATTAA CGGCGATAAA 1651 GGCTATAACG GTCTCGCTGA AGTCGGTAAG AAATTCGAGA AAGATACCGG 1701 AATTAAAGTC ACCGTTGAGC ATCCGGATAA ACTGGAAGAG AAATTCCCAC 1751 AGGTTGCGGC AACTGGCGAT GGCCCTGACA TTATCTTCTG GGCACACGAC 1801 CGCTTTGGTG GCTACGCTCA ATCTGGCCTG TTGGCTGAAA TCACCCCGGA 1851 CAAAGCGTTC CAGGACAAGC TGTATCCGTT TACCTGGGAT GCCGTACGTT 1901 ACAACGGCAA GCTGATTGCT TACCCGATCG CTGTTGAAGC GTTATCGCTG 1951 ATTTATAACA AAGATCTGCT GCCGAACCCG CCAAAAACCT GGGAAGAGAT 2001 CCCGGCGCTG GATAAAGAAC TGAAAGCGAA AGGTAAGAGC GCGCTGATGT 2051 TCAACCTGCA AGAACCGTAC TTCACCTGGC CGCTGATTGC TGCTGACGGG 2101 GGTTATGCGT TCAAGTATGA AAACGGCAAG TACGACATTA AAGACGTGGG 2151 CGTGGATAAC GCTGGCGCGA AAGCGGGTCT GACCTTCCTG GTTGACCTGA 2201 TTAAAAACAA ACACATGAAT GCAGACACCG ATTACTCCAT CGCAGAAGCT 2251 GCCTTTAATA AAGGCGAAAC AGCGATGACC ATCAACGGCC CGTGGGCATG 2301 GTCCAACATC GACACCAGCA AAGTGAATTA TGGTGTAACG GTACTGCCGA 2351 CCTTCAAGGG TCAACCATCC AAACCGTTCG TTGGCGTGCT GAGCGCAGGT 2401 ATTAACGCCG CCAGTCCGAA CAAAGAGCTG GCAAAAGAGT TCCTCGAAAA 2451 CTATCTGCTG ACTGATGAAG GTCTGGAAGC GGTTAATAAA GACAAACCGC 2501 TGGGTGCCGT AGCGCTGAAG TCTTACGAGG AAGAGTTGGC GAAAGATCCA 2551 CGTATTGCCG CCACTATGGA AAACGCCCAG AAAGGTGAAA TCATGCCGAA 2601 CATCCCGCAG ATGTCCGCTT TCTGGTATGC CGTGCGTACT GCGGTGATCA 2651 ACGCCGCCAG CGGTCGTCAG ACTGTCGATG AAGCCCTGAA AGACGCGCAG 2701 ACTAATTCGA GCTCGAACAA CAACAACAAT AACAATAACA ACAACCTCGG 2751 GATCGAGGGA AGGATTTCAG AATTCGGATC CTCTAGAGTC GACCTGCAGG 2801 CAAGCTTGGC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT 2851 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG 2901 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA 2951 GCCTGAATGG CGAATGGCAG CTTGGCTGTT TTGGCGGATG AGATAAGATT 3001 TTCAGCCTGA TACAGATTAA ATCAGAACGC AGAAGCGGTC TGATAAAACA 3051 GAATTTGCCT GGCGGCAGTA GCGCGGTGGT CCCACCTGAC CCCATGCCGA 3101 ACTCAGAAGT GAAACGCCGT AGCGCCGATG GTAGTGTGGG GTCTCCCCAT 3151 GCGAGAGTAG GGAACTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA 3201 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG 3251 AGTAGGACAA ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC 3301 CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG CATCAAATTA 3351 AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT ACAAACTCTT 3401 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 3451 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 3501 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 3551 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 3601 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 3651 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTCTCCAATG ATGAGCACTT 3701 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA 3751 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 3801 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 3851 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 3901 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 3951 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 4001 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 4051 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 4101 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 4151 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 4201 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 4251 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 4301 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 4351 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTACCCCG 4401 GTTGATAATC AGAAAAGCCC CAAAAACAGG AAGATTGTAT AAGCAAATAT 4451 TTAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT 4501 AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT 4551 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA 4601 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA 4651 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CAAATCAAGT 4701 TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 4751 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG 4801 AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG 4851 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA 4901 GGGCGCGTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 4951 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 5001 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT 5051 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 5101 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 5151 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 5201 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 5251 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 5301 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT 5351 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 5401 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 5451 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 5501 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 5551 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 5601 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT 5651 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 5701 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 5751 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC 5801 GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT 5851 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA 5901 CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA 5951 ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA 6001 CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC 6051 CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG 6101 TCGTGCAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT 6151 GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT 6201 TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA 6251 TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC 6301 GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG 6351 GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG 6401 GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG 6451 CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG 6501 ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT 6551 GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG 6601 CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC 6651 CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG 6701 GACCCAACGC TGCCCGAAAT T