!!NA_SEQUENCE 1.0 Plasmid pRS416 Update 6/3/02 Comments: This sequence differs slightly from the published pRS416 sequence (GenBank U03450) in the region upstream of URA3 (nt 191-247 of the published sequence and nt 191-240 of the sequence below). Sequencing of this region at NEB in 2002 confirms the sequence below. Features: 410-1213 URA3 CDS (start 410) 1799-1344 f1 origin of replication (+ -) 2160-1582 lacZ alpha CDS/multiple cloning site (start 2160, complementary strand) 3145-2558 pMB1 origin of replication (counterclockwise) (RNAII -35 to RNA/DNA switch point) [NOTE: rop gene has been disrupted.] 4177-3317 beta-lactamase (bla; amp-r) CDS (start 4177, complementary strand) 4177-4109 beta-lactamase signal peptide CDS (start 4177, complementary strand) 4318-4436 CEN6 (yeast centromere sequence) 4449-4822 Histone H4 ARS (autonomously replicating sequence) prs416.seq Length: 4891 June 3, 2002 13:25 Type: N Check: 2273 .. 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG 51 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 101 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 151 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATACCAC CTTTTCAATT 201 CATCATTTTT TTTTTATTCT TTTTTTTGAT TTCGGTTTCC TTGAAATTTT 251 TTTGATTCGG TAATCTCCGA ACAGAAGGAA GAACGAAGGA AGGAGCACAG 301 ACTTAGATTG GTATATATAC GCATATGTAG TGTTGAAGAA ACATGAAATT 351 GCCCAGTATT CTTAACCCAA CTGCACAGAA CAAAAACCTG CAGGAAACGA 401 AGATAAATCA TGTCGAAAGC TACATATAAG GAACGTGCTG CTACTCATCC 451 TAGTCCTGTT GCTGCCAAGC TATTTAATAT CATGCACGAA AAGCAAACAA 501 ACTTGTGTGC TTCATTGGAT GTTCGTACCA CCAAGGAATT ACTGGAGTTA 551 GTTGAAGCAT TAGGTCCCAA AATTTGTTTA CTAAAAACAC ATGTGGATAT 601 CTTGACTGAT TTTTCCATGG AGGGCACAGT TAAGCCGCTA AAGGCATTAT 651 CCGCCAAGTA CAATTTTTTA CTCTTCGAAG ACAGAAAATT TGCTGACATT 701 GGTAATACAG TCAAATTGCA GTACTCTGCG GGTGTATACA GAATAGCAGA 751 ATGGGCAGAC ATTACGAATG CACACGGTGT GGTGGGCCCA GGTATTGTTA 801 GCGGTTTGAA GCAGGCGGCA GAAGAAGTAA CAAAGGAACC TAGAGGCCTT 851 TTGATGTTAG CAGAATTGTC ATGCAAGGGC TCCCTATCTA CTGGAGAATA 901 TACTAAGGGT ACTGTTGACA TTGCGAAGAG CGACAAAGAT TTTGTTATCG 951 GCTTTATTGC TCAAAGAGAC ATGGGTGGAA GAGATGAAGG TTACGATTGG 1001 TTGATTATGA CACCCGGTGT GGGTTTAGAT GACAAGGGAG ACGCATTGGG 1051 TCAACAGTAT AGAACCGTGG ATGATGTGGT CTCTACAGGA TCTGACATTA 1101 TTATTGTTGG AAGAGGACTA TTTGCAAAGG GAAGGGATGC TAAGGTAGAG 1151 GGTGAACGTT ACAGAAAAGC AGGCTGGGAA GCATATTTGA GAAGATGCGG 1201 CCAGCAAAAC TAAAAAACTG TATTATAAGT AAATGCATGT ATACTAAACT 1251 CACAAATTAG AGCTTCAATT TAATTATATC AGTTATTACC CTATGCGGTG 1301 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT 1351 AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT 1401 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA 1451 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC 1501 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC 1551 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG 1601 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT 1651 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA 1701 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG 1751 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC 1801 GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC 1851 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG 1901 CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC 1951 GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC GAATTGGGTA 2001 CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC 2051 CTGCAGCCCG GGGGATCCAC TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA 2101 GCTCCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA 2151 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 2201 ACACAACATA GGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 2251 GAGTGAGGTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG 2301 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 2351 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 2401 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 2451 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 2501 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 2551 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 2601 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 2651 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 2701 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 2751 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 2801 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 2851 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 2901 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 2951 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 3001 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 3051 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 3101 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 3151 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 3201 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 3251 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 3301 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 3351 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 3401 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 3451 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 3501 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 3551 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 3601 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 3651 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG 3701 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 3751 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 3801 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 3851 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 3901 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 3951 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG 4001 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 4051 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 4101 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 4151 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 4201 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 4251 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 4301 GCCACCTGGG TCCTTTTCAT CACGTGCTAT AAAAATAATT ATAATTTAAA 4351 TTTTTTAATA TAAATATATA AATTAAAAAT AGAAAGTAAA AAAAGAAATT 4401 AAAGAAAAAA TAGTTTTTGT TTTCCGAAGA TGTAAAAGAC TCTAGGGGGA 4451 TCGCCAACAA ATACTACCTT TTATCTTGCT CTTCCTGCTC TCAGGTATTA 4501 ATGCCGAATT GTTTCATCTT GTCTGTGTAG AAGACCACAC ACGAAAATCC 4551 TGTGATTTTA CATTTTACTT ATCGTTAATC GAATGTATAT CTATTTAATC 4601 TGCTTTTCTT GTCTAATAAA TATATATGTA AAGTACGCTT TTTGTTGAAA 4651 TTTTTTAAAC CTTTGTTTAT TTTTTTTTCT TCATTCCGTA ACTCTTCTAC 4701 CTTCTTTATT TACTTTCTAA AATCCAAATA CAAAACATAA AAATAAATAA 4751 ACACAGAGTA AATTCCCAAA TTATTCCATC ATTAAAAGAT ACGAGGCGCG 4801 TGTAAGTTAC AGGCAAGCGA TCCGTCCTAA GAAACCATTA TTATCATGAC 4851 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT C