New England Biolabs Web Tools
Restriction Enzymes Tools
Double Digest Finder - buffer and reaction conditions for digests requiring two restriction enzymes.
|Enzyme Finder - search for a restriction enzyme by category, recognition sequence, or name to find information on any NEB enzyme. |
|NEBCutter - generate restriction maps of your own sequence, view gels, ORF summary and more . . . |
|REBASE - the Restriction Enzyme Database.|
Quick and easy access to the most requested restriction enzyme information allows you to plan your experiments from anywhere. Sort your results from Enzyme Finder so they make sense to you, then email them to your inbox or connect directly to www.neb.com. Determine buffer and reaction conditions for experiments requiring two restriction enzymes using the Double Digest Finder.
|PCR Selection Tool - use this tool to help you choose the best polymerase for your PCR application.|
|Tm Calculator - estimate the optimal annealing temperature for PCR reactions|
|DNA Sequences and Maps Tool - sequence files used to produce the plasmid vector, viral and bacteriophage maps in the NEB Catalogue and the tables containing the locations of sites.|
|InBase - a curated database devoted to inteins.|
|NEBase Changer - provides custom primer sequences and annealing temperatures to be used with the Q5® Site-Directed Mutagenesis Kit (E0554).|
|NEBuilder - design primers for Gibson Assembly reactions, based on the entered fragment sequences and the polymerase being used for amplification.|
|PolBase - a repository of biochemical, genetic, and structural information about DNA Polymerases.|