NEBNext™ Sample Preparation

• Overview
• Advantages
• Workflow
• Products
• Press Release: Validation of NEBNext™ Reagents by Ontario Institute for Cancer Research

Introducing NEBNext, a series of highly pure reagents that facilitate sample preparation of DNA for downstream applications such as next generation sequencing, and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

NEBNext™ dsDNA Fragmentase™ is an enzyme-based solution to the fragmentation of DNA. An alternative to nebulization, dsDNA Fragmentase will generate 100-800 bp for fragments in a time dependent manner, and is ideal for generation of expression libraries or next-generation sequencing.

Advantages of NEBNext Sample Prep Reagents:

• Convenient formats – All of the required enzymes, buffers and nucleotides are included, and are also available in Master Mix format. Modules offer the ability to customize sample preparation.
• Functional Validation – Each set of reagents or module is functionally validated by preparation of a genomic DNA library that is sequenced using the Illumina® GAII and by preparation of an expression library.
• Stringent Quality Controls – Additional QCs ensure maximum quality and purity.
• Value Pricing

Advantages of NEBNext dsDNA Fragmentase:

• High throughput compatibility
• Control of fragment size
• Generates fragments in the 100-300 base pair range more effectively than nebulization
• Enzyme-based cleavage results in cleaner ends as compared to mechanical shearing
• Value pricing

Genomic Library Construction Workflow

NEBNext offers a solution for each step of Library Construction Workflow.

Sequencing coverage map of the E. coli genome after using NEBNext DNA SPRS1 for Sample Preparation

E. coli strain MG1655 gDNA was prepared with NEBNext Sample Prep Reagent Set I and sequenced on an Illumina Genome Analyzer II.

NEBNext dsDNA Fragmentase generates fragments in the 100–300 bp range more effectively than nebulization

Relative size distribution of E. coli DNA fragments with dsDNA Fragmentase vs. Nebulization as seen using the Bioanalyzer 2100. The dsDNA Fragmentase sample was incubated for 30 minutes at 37°C with 0.05 µg of DNA per µl of dsDNA Fragmentase in 1X Fragmentase Reaction Buffer with 100 µg/ml BSA. The Nebulizer sample was prepared by nebulization of DNA in 50% glycerol for 6 minutes at 35 psi.

Technical Reference Quick Links
Reagents for Nucleic Acid Sample Preparation

FAQs for NEBNext Products

Construction of an Expression Library

FAQs for Fragmentase

Protocols for Fragmentase

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