NEB® 10-beta Competent E. coli (High Efficiency)

#C3019

Catalog # Size Price (£)
C3019H 20 X 0.05 ML £198.00
C3019P 1 96-WELL PLATE £500.00
C3019I 6 X 0.2 ML £165.00

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Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights

  • DH10B™ derivative

  • Transformation efficiency:
         For C3019I and C3019H:  1-3 x 109 cfu/µg pUC19 DNA
         For C3019P: 1-3 x 108 cfu/µg pUC19 DNA 

  • Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]

  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations

  • Resistance to phage T1 (fhuA)

  • Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene

  • Reduced recombination of cloned DNA (recA1)

  • Clone large plasmids and BACs

  • Free of animal products

  • K12 Strain

Genotype

Δ(ara-leu) 7697 araD139  fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC) 

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • C3019H

     

     

    -80

     

     

  • C3019I

     

     

    -80

     

     

  • C3019P

     

     

    -80

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



Legal and Disclaimers



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