NEB® 5-alpha Competent E. coli (High Efficiency)

#C2987

Catalog # Size Price (£)
C2987H 20 X 0.05 ML £180.00
C2987R 1 384-WELL PLATE £989.00
C2987P 1 96-WELL PLATE £456.00
C2987I 6 X 0.2 ML £151.00
C2987U 96 X 0.05 ML £667.00

NEB 5-alpha Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. 

  • High efficiency strain ideal for a wide variety of applications

  • Available in a wide variety of sizes, including single-use vials, 200 μl vials, 96- and 384- well plate and 12 x 8 tube strips

  • No dry ice surcharge on competent cell shipments

  • Outgrowth medium included

  • Free of animal products

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications. Available in 5 convenient formats:

  • Single-use 50 μl vials

  • 200 μl vials

  • 96-well plate

  • 384-well plate

  • 12 x 8-tube strips: packed in 12 x 8-Strip Microplate Cluster Tube (Costar™ Cluster Tube System)

 

For C2987H, C2987I, C2987P and C2987U, SOC Outgrowth Medium is provided.
For C2987R, Outgrowth Medium 1.5 is provided.

Highlights

  • DH5α™ derivative

  • Free of animal products

  • Transformation efficiency:
    For C2987H, C2987I, C2987P and C2987U: 1 - 3 x 109 cfu/μg pUC19 DNA
    For C2987R: 1 - 5 x 108 cfu/μg pUC19 DNA

  • Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)

  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations

  • Resistance to phage T1 (fhuA2)

  • Suitable for blue/white screening by α-complementation of the β-galactosidase gene

  • Reduced recombination of cloned DNA (recA1)

  • K12 Strain

Genotype

fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • C2987H

     

     

    -80

     

     

  • C2987I

     

     

    -80

     

     

  • C2987P

     

     

    -80

     

     

  • C2987R

     

     

    -80

     

     

  • C2987U

     

     

    -80

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

FAQs

Protocols

Datacards

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



Datacards

Legal and Disclaimers



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