NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

#C2992

Catalog # Size Price (£)
C2992H 20 X 0.05 ML £185.00
C2992I 6 X 0.2 ML £149.00

NEB 5-alpha F'Iq Competent E. coli is a derivative of the popular DH5α.

  • Extremely high transformation efficiency suitable for toxic gene cloning

  • Tight expression control (lacIq)

  • No dry ice surcharge on competent cell shipments

  • Outgrowth medium included

  • Free of animal products

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights

  • DH5α™ derivative

  • Free of animal products

  • Transformation efficiency 1-3 x 109 cfu/µg pUC19 DNA

  • Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)

  • Tight control of expression by laclq allows potentially toxic genes to be cloned

  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations

  • Resistance to phage T1 (fhuA2)

  • Suitable for blue/white screening by α-complementation of the β-galactosidase gene

  • F' allows cells to be infected with bacteriophage M13 for ssDNA production

  • Reduced recombination of cloned DNA (recA1)

  • K12 Strain

Genotype

proA B lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) / fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • C2992H

     

     

    -80

     

     

  • C2992I

     

     

    -80

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



Datacards

Legal and Disclaimers



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