NEB® Turbo Competent E. coli (High Efficiency)

#C2984

Catalog # Size Price (£)
C2984H 20 X 0.05 ML £237.00
C2984I 6 X 0.2 ML £201.00

E. coli cells featuring fast colony growth (6.5 hours)

  • Tight expression control (lacIq)

  • Isolate DNA after only 4 hours of growth

  • 5-minute transformation protocol with AmpR plasmids

  • No dry ice surcharge on competent cell shipments

  • Outgrowth medium included

  • Free of animal products

Chemically competent E. coli cells suitable for high efficiency transformation and rapid colony growth.

Highlights

  • Transformation efficiency: 1 - 3 x 109 cfu/μg pUC19 DNA

  • Tight control of expression by laclq allows potentially toxic genes to be cloned

  • Highest growth rate on agar plates - visible colonies 6.5 hours after transformation

  • Activity nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations

  • Resistance to phage T1 (fhuA2)

  • Suitable for blue/white screening by α-complementation of the β-galactosidase gene

  • Suitable for 5 minute transformation protocol with AmpR plasmids

  • EcoKr-m-, McrBC-

  • K12 Strain

  • Isolate DNA after 4 hours of growth from a single overnight colony

  • Free of animal products

Genotype

F' proA B lacIq ∆lacZM15 / fhuA2  ∆(lac-proAB)  glnV galK16 galE15  R(zgb-210::Tn10)TetS  endA1 thi-1 ∆(hsdS-mcrB)5  

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • C2984H

     

     

    -80

     

     

  • C2984I

     

     

    -80

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

FAQs

Protocols

Datacards

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



Datacards

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