Bst 2.0 WarmStart® DNA Polymerase

#M0538

cloned at NEB recombinant NEBU 65 80 Heat hotstart
Catalog # Size Concentration Price (£)
M0538L 8000 UNITS 8,000 units/ml £288.00
M0538S 1600 UNITS 8,000 units/ml £74.00
M0538M 8000 UNITS 120,000 units/ml £288.00

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Optimized for Loop-Mediated Isothermal Amplification (LAMP)

  • Consistent amplification performance with the convenience of room temperature setup

  • WarmStart® technology eliminates off-target amplifilcation and offers increased reaction efficiency

  • Optimal reaction performance from 60-72 °C




Bst

2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.

Benefits of Bst 2.0 WarmStart Benefits of Bst 2.0 WarmStart
Identical LAMP reactions were run either immediately after setup (solid line) or after a 2 hour incubation at 25 °C. Without the protection from Bst 2.0 WarmStart, this room temperature incubation results in variable LAMP performance. Bst 2.0 WarmStart provides more consistent amplification reaction and enables room-temperature and high-throughput setup.

Product Source

Bst

2.0 WarmStart DNA Polymerase is prepared from an E. coli strain that expresses the Bst 2.0 DNA Polymerase protein from an inducible promoter.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

Advantages and Features

Properties & Usage

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Product Notes

Protocols

Datacards

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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