WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

#M1800

cloned at NEB recombinant 65 hotstart
Catalog # Size Concentration Price (£)
M1800L 500 REACTIONS 2 X £865.00
M1800S 100 REACTIONS 2 X £216.00

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Fast, clear pink-to-yellow visible detection of amplification

  • WarmStart® feature inhibits activity at room temperature

  • Learn how Colorimetric LAMP was used for rapid detection of coronavirus (COVID-19) RNA in our recent publication

  • Offered with dUTP and UDG for carryover contamination prevention (NEB #M1804)

  • Learn more

    about how RT-LAMP is being used for rapid COVID-19 screening



Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.


The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes. 

Simple Visualization of Amplification Success

Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • M1800S

     

     

    -20

     

     

  • M1800L

     

     

    -20

     

     

Advantages and Features

Properties & Usage

Related Products

FAQs

Quality Control Assays

Certificate of Analysis

Change Notifications

Product is now supplied in Sarstedt vials (February 28, 2022).

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



Legal and Disclaimers



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