WarmStart® Colorimetric LAMP 2X Master Mix with UDG

#M1804

cloned at NEB recombinant 65 hotstart
Catalog # Size Concentration Price (£)
M1804L 500 REACTIONS 2 X £1019.00
M1804S 100 REACTIONS 2 X £255.00

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Fast, clear, pink-to-yellow visible detection of amplification

  • Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix

  • WarmStart® feature inhibits enzyme activity at room temperature allowing for flexible reaction setup

  • Learn more

    about LAMP and other isothermal amplification methods

  • Learn how

    colorimetric LAMP is being used in COVID-19 research

  • Learn more

    about how RT-LAMP is being used for rapid COVID-19 screening



Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.


WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions.

This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in the Featured Videos section). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.

Simple Visualization of Amplification Success

Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • M1804S

     

     

    -20

     

     

  • M1804L

     

     

    -20

     

     

Advantages and Features

Properties & Usage

Related Products

FAQs

Selection Charts

Selection Charts

Quality Control Assays

Certificate of Analysis

Change Notifications

Product is now supplied in Sarstedt vials (February 28, 2022).

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



Legal and Disclaimers



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