WarmStart® LAMP Kit (DNA & RNA)

#E1700

Catalog # Size Price (£)
E1700L 500 REACTIONS £867.00
E1700S 100 REACTIONS £216.00
  • Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Compatible with multiple detection methods

  • WarmStart® technology inhibits activity at room temperature

  • This reagent has been incorporated into Oxford Nanopore's LamPORE technology, a combination of LAMP with nanopore sequencing, initially implemented to provide a rapid, low-cost and scalable assay for the detection of SARS-CoV-2.

  • Learn more

    about how RT-LAMP is being used for rapid COVID-19 screening

  • LAMP Fluorescent Dye (NEB #B1700) component available separately


The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

The WarmStart LAMP Kit (DNA & RNA) is compatible with multiple detection methods


The WarmStart LAMP Kit (DNA & RNA) includes fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or a variety of endpoint visualization methods.

For carryover contamination prevention, a version containing dUTP and thermolabile UDG is available: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) (NEB #E1708).

NEB's WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity


speed and sensitivity
A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time thermocycler (Bio-Rad CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. NEB's WarmStart LAMP Kit resulted in faster and more robust detection as compared to the OptiGene Master Mix.

NEB's WarmStart LAMP Kit (DNA & RNA) provides fast LAMP/RT-LAMP results


Fast LAMP/RT-LAMP results
Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. Lucigen® OmniAmp® RNA & DNA Kit and OptiGene Isothermal Master Mix (ISO-001 ) were run according to manufacturers' recommendations.

NEB WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets

PRIMER SET

DNA TEMPLATE

HeLa 
DNA

Lyme 
DNA

B. malayi 
DNA

E. coli
DNA

C. elegans 
DNA

BRCAb (human)

 

 

 

 

CFTR (human)

 

 

 

 

LymO (Lyme)

 

 

 

 

BmHhal (B. malayi)

 

 

 

 

dnaE (E. coli)

 

 

 

 

Lec10b (C. elegans)

 

 

 

 

Lec6 (C. elegans)

 

 

 

 

In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a thermocycler (Bio-Rad CFX96) in triplicate. Positive results ( ) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.

Kit Components

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • E1700S

     

     

    -20

     

     

  • E1700L

     

     

    -20

     

     

Properties & Usage

Related Products

References

Protocols

Manuals

Quality Control Assays

Certificate of Analysis

Change Notifications

The M1700 component is now supplied in Sarstedt vials (February 28, 2022).

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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