WarmStart® LAMP Kit (DNA & RNA)#E1700

Catalog # Size Price (£)
E1700L 500 reactions £773.00
E1700S 100 reactions £193.00
Description

The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

NEB's WarmStart LAMP Kit (DNA & RNA) is compatible with multiple detection methods


The NEB WarmStart LAMP Kit (DNA & RNA) includes separate fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or endpoint visualization.

NEB's WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity


speed and sensitivity
A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time thermocycler (Bio-Rad CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. NEB's WarmStart LAMP Kit resulted in faster and more robust detection as compared to the OptiGene Master Mix.

NEB's WarmStart LAMP Kit (DNA & RNA) provides fast LAMP/RT-LAMP results


Fast LAMP/RT-LAMP results
Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. Lucigen® OmniAmp® RNA & DNA Kit and OptiGene Isothermal Master Mix (ISO-001 ) were run according to manufacturers' recommendations.

NEB WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets

PRIMER SET

DNA TEMPLATE

HeLa 
DNA

Lyme 
DNA

B. malayi 
DNA

E. coli
DNA

C. elegans 
DNA

BRCAb (human)

 

 

 

 

CFTR (human)

 

 

 

 

LymO (Lyme)

 

 

 

 

BmHhal (B. malayi)

 

 

 

 

dnaE (E. coli)

 

 

 

 

Lec10b (C. elegans)

 

 

 

 

Lec6 (C. elegans)

 

 

 

 

In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a thermocycler (Bio-Rad CFX96) in triplicate. Positive results ( ) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.

Kit Components

The following reagents are supplied with this product:

Store at (°C)

Concentration

WarmStart LAMP 2X Master Mix

-20

LAMP Fluorescent Dye (50X)

-20


Properties and Usage

Materials Required but not Supplied

  • LAMP Primers (we recommend using PrimerExplorer for design of optimal LAMP Primers; PrimerExplorer V5 is recommended, https://primerexplorer.jp, though requiring browser translation from Japanese; V4 in English accessible via http://primerexplorer.jp/e)

  • Target Nucleic Acid Samples

  • Molecular Biology Grade H2O

  • Heat block, water bath, real-time turbidimeter or thermocycler (with real-time fluorescence measurement if desired) and instrument-appropriate reaction vessels

Storage Temperature

-20°C


Related Products

Companion Products


Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]


Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.



This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

Licensed under U.S. Patents Nos. U.S. 6,410,278; 6,974,670; 7,494,790; 8,017,357; 7,374,913; 7,851,186; and 7,846,695 including all foreign counterparts thereof. The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased product to perform loop-mediated isothermal amplification ("LAMP") for Research Use Only. The purchase of this product further conveys to the purchaser the limited, non-transferable right to use the purchased product to perform reverse transcription loop-mediated isothermal amplification ("RT-LAMP") for Research Use Only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd and any use other than research may require a license from Eiken Chemical Co., Ltd. Notice to Purchaser: Nucleic-acid based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. (See Patent Nos. 5,475,096; 5,670,637; 5,696,249; 5,874,557; and 5,693,502). New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the aptamer-based RT-LAMP Master Mix for Research Purposes Only. Commercial use of the aptamer-based RT-LAMP Master Mix requires a license from New England Biolabs, Inc. Please contact busdev@neb.com for more information.



ADDED