Blunt/TA Ligase Master Mix

#M0367

cloned at NEB recombinant No
Catalog # Size Concentration Price (£)
M0367L 250 REACTIONS 2 X £402.00
M0367S 50 REACTIONS 2 X £101.00

Blunt/TA Ligase Master Mix will ligate these substrates:

dsDNA




Nicked DNA/RNA





 


Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates.

  • Simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components

  • No thawing is necessary as it remains liquid during storage at -20°C

  • Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution

  • Suitable for Adaptor Ligation

  • Compatible with several Oxford Nanopore Technologies® workflows, including some involving Covid-19 research

  • Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Hi-T4 and Instant Sticky-end Ligase Master Mix

  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. The master mix format simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components, and yields robust, rapid ligation of all types of DNA ends using a short incubation time at room temperature. No thawing is necessary as it remains liquid during storage at -20°C.* Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.

Learn more about Joining Difficult to Ligase dsDNA Fragments and Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using Blunt/TA Ligase Master Mix.

Blunt/TA Ligase Master Mix improves yields for ends that typically react slowly


Yields of final ligation product for all reaction conditions using high concentration T4 DNA Ligase (NEB #M0202), the Quick Ligation Kit (NEB #M2200) and Blunt/TA Master Mix (NEB #M0367). Nick, cohesive end and 3´ single-base overhang substrates were incubated for 15 minutes; the 5´ single-base overhang was incubated for 1 hour. * Freezers vary in their actual internal temperature. Our testing demonstrates that the master mix is liquid at -20°C. Freeze-thaw testing at -70°C has confirmed that the performance is unchanged after 20 freeze/thaw cycles.

M0367 Ligation reactions with single-base overhang vector and insert were set-up using the Blunt/TA Ligase Master Mix and incubated for different times at 25°C (A) or at different temperatures for 15 minutes (B). Two microliters of each reaction were used to transform a 50 μl aliquot of NEB 10-beta Competent E. coli (NEB #C3019). Transformants resulting from triplicate plating 50 μl of a 1:5 dilution of the outgrowth were counted and graphed. The results indicate that the Blunt/TA Ligase Master Mix works well at 25°C, and is complete in 15 minutes.

Product Source

Purified from an E. coli strain containing a recombinant gene encoding T4 DNA Ligase.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

  • M0367S

     

     

    -20

     

     

  • M0367L

     

     

    -20

     

     

Advantages and Features

Properties & Usage

Related Products

Product Notes

References

Protocols

Datacards

Quality Control Assays

Certificate of Analysis

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

The following link is to a PDF Safety Data Sheet (SDS) that applies to this product to help you use it safely.



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